(Circular) (Six-base) MAPSORT of: pFN21AE6784.seq Check: 4945 from: 1 to: 6589 pFN21AE6784 6589 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2640 3687 3932 1937 Size: 703 1047 245 4594 Fragments arranged by size: 4594 1047 703 245 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3124 3509 4652 6036 6304 1632 Size: 1492 385 1143 1384 268 1917 Fragments arranged by size: 1917 1492 1384 1143 385 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 2611 5488 6465 1203 Size: 1408 2877 977 1327 Fragments arranged by size: 2877 1408 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6589 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AlwNI CAG_nnn'CTG Cuts at: 2408 4332 5904 2408 Size: 1924 1572 3093 Fragments arranged by size: 3093 1924 1572 ApaI G_GGCC'C Cuts at: 2231 2517 2231 Size: 286 6303 ApaLI G'TGCA_C Cuts at: 2595 4646 5802 2595 Size: 2051 1156 3382 Fragments arranged by size: 3382 2051 1156 ApoI r'AATT_y Cuts at: 1493 3898 4124 4276 6385 1493 Size: 2405 226 152 2109 1697 Fragments arranged by size: 2405 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3048 5143 160 Size: 872 2016 2095 1606 Fragments arranged by size: 2095 2016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 2083 2343 2500 3785 3914 1870 Size: 73 140 260 157 1285 129 4545 Fragments arranged by size: 4545 1285 260 157 140 129 73 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2326 3115 3910 5365 Size: 325 217 627 248 291 789 795 1455 Cuts at: 5365 618 Size: 1842 Fragments arranged by size: 1842 1455 795 789 627 325 291 248 217 BanII G_rGCy'C Cuts at: 1391 2133 2231 2517 3908 1391 Size: 742 98 286 1391 4072 Fragments arranged by size: 4072 1391 742 286 98 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5618 BbvCI CC'TCA_GC Cuts at: 2135 2135 Size: 6589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2288 4481 5691 2288 Size: 2193 1210 3186 Fragments arranged by size: 3186 2193 1210 BclI T'GATC_A Cuts at: 1548 3061 1548 Size: 1513 5076 BfrBI ATG'CAT Cuts at: 3750 3750 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5200 136 Size: 107 122 71 631 605 3528 1525 Fragments arranged by size: 3528 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6584 6584 Size: 6589 BlpI GC'TnA_GC Cuts at: 4006 4006 Size: 6589 Bme1580I G_kGCm'C Cuts at: 1790 2231 2329 2517 2599 3120 3335 4650 5806 Size: 441 98 188 82 521 215 1315 1156 Cuts at: 5806 1790 Size: 2573 Fragments arranged by size: 2573 1315 1156 521 441 215 188 98 82 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4320 5278 442 Size: 416 53 83 399 24 2903 958 1753 Fragments arranged by size: 2903 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 5249 1239 Size: 1490 2520 2579 Fragments arranged by size: 2579 2520 1490 Bpu10I CC'TnA_GC Cuts at: 2135 2730 3175 2135 Size: 595 445 5549 Fragments arranged by size: 5549 595 445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2286 2898 4714 5579 5877 2286 Size: 612 1816 865 298 2998 Fragments arranged by size: 2998 1816 865 612 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5252 882 Size: 617 3753 2219 Fragments arranged by size: 3753 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2614 4380 493 Size: 711 1410 1766 2702 Fragments arranged by size: 2702 1766 1410 711 BsaBI GATnn'nnATC Cuts at: 2778 6583 2778 Size: 3805 2784 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 4396 4778 5413 Size: 53 83 186 936 503 2360 382 635 Cuts at: 5413 275 Size: 1451 Fragments arranged by size: 2360 1451 936 635 503 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3185 5022 5420 5694 5841 6252 3185 Size: 1837 398 274 147 411 3522 Fragments arranged by size: 3522 1837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6364 Fragments arranged by size: 6364 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 1186 Size: 906 5683 BseYI C'CCAG_C Cuts at: 2046 2121 5792 6288 2046 Size: 75 3671 496 2347 Fragments arranged by size: 3671 2347 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2266 3617 1163 Size: 573 530 1351 4135 Fragments arranged by size: 4135 1351 573 530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2256 4039 4800 5828 1540 Size: 275 186 255 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 255 186 BsiHKAI G_wGCw'C Cuts at: 2460 2599 3597 4650 4735 5806 2460 Size: 139 998 1053 85 1071 3243 Fragments arranged by size: 3243 1071 1053 998 139 85 BsmI GAATG_Cn' Cuts at: 4100 4193 4100 Size: 93 6496 BsmBI CGTCTCn'nnnn_ Cuts at: 3782 3782 Size: 6589 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2231 2329 2460 2517 2599 3120 Size: 399 343 98 98 131 57 82 521 Cuts at: 3120 3335 3597 3908 4650 4735 5806 1391 Size: 215 262 311 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 521 399 343 311 262 215 131 98 98 85 82 57 BspEI T'CCGG_A Cuts at: 6252 6252 Size: 6589 BspHI T'CATG_A Cuts at: 4478 6208 4478 Size: 1730 4859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2288 3944 844 Size: 1444 1656 3489 Fragments arranged by size: 3489 1656 1444 BsrBI CCG'CTC Cuts at: 1394 1823 2088 4476 1394 Size: 429 265 2388 3507 Fragments arranged by size: 3507 2388 429 265 BsrDI GCAATG_nn' Cuts at: 65 1248 3750 3846 5084 5258 65 Size: 1183 2502 96 1238 174 1396 Fragments arranged by size: 2502 1396 1238 1183 174 96 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 2285 5233 5420 1700 Size: 110 445 30 2948 187 2869 Fragments arranged by size: 2948 2869 445 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 3386 4649 5661 3386 Size: 1263 1012 4314 Fragments arranged by size: 4314 1263 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6589 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstYI r'GATC_y Cuts at: 1972 3919 4286 4671 4688 6140 6226 6238 6584 Size: 1947 367 385 17 1452 86 12 346 Cuts at: 6584 1972 Size: 1977 Fragments arranged by size: 1977 1947 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2590 2590 Size: 6589 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2199 2391 513 Size: 552 939 66 129 192 4711 Fragments arranged by size: 4711 939 552 192 129 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3017 487 Size: 683 56 1791 4059 Fragments arranged by size: 4059 1791 683 56 BtsI GCAGTG_nn' Cuts at: 4105 4899 4919 4105 Size: 794 20 5775 Fragments arranged by size: 5775 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 3373 3893 3373 Size: 520 6069 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2253 2530 Size: 54 1425 50 275 18 282 141 277 Cuts at: 2530 2632 2872 4036 4925 6554 8 Size: 102 240 1164 889 1629 43 Fragments arranged by size: 1629 1425 1164 889 282 277 275 240 141 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2253 4036 1537 Size: 275 441 1783 4090 Fragments arranged by size: 4090 1783 441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3501 4519 1353 Size: 2148 1018 3423 Fragments arranged by size: 3423 2148 1018 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5176 5550 5696 387 Size: 1064 138 3587 374 146 1280 Fragments arranged by size: 3587 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2729 3124 3509 4652 5249 6036 6304 Size: 393 1097 395 385 1143 597 787 268 Cuts at: 6304 1239 Size: 1524 Fragments arranged by size: 1524 1143 1097 787 597 395 393 385 268 EcoO109I rG'GnC_Cy Cuts at: 2227 2513 2514 2227 Size: 286 1 6302 Fragments arranged by size: 6302 286 1 EcoRI G'AATT_C Cuts at: 3898 4276 3898 Size: 378 6211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3530 3530 Size: 6589 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2039 5736 1678 Size: 361 3697 2531 Fragments arranged by size: 3697 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2084 2116 3046 Size: 32 135 32 479 32 1 32 930 Cuts at: 3046 3078 3584 3616 3680 3712 5230 5262 5304 Size: 32 506 32 64 32 1518 32 42 Cuts at: 5304 5336 1373 Size: 32 2626 Fragments arranged by size: 2626 1518 930 506 479 135 64 42 32 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2167 2655 2695 3933 4179 669 Size: 988 281 229 488 40 1238 246 3079 Fragments arranged by size: 3079 1238 988 488 281 246 229 40 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 2035 2035 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 3282 3306 3852 4188 3282 Size: 24 546 336 5683 Fragments arranged by size: 5683 546 336 24 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5703 5887 1305 Size: 4398 184 2007 Fragments arranged by size: 4398 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2532 2634 2874 10 Size: 54 1425 343 700 102 240 3725 Fragments arranged by size: 3725 1425 700 343 240 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 2616 4547 4906 5065 518 Size: 648 1450 1931 359 159 2042 Fragments arranged by size: 2042 1931 1450 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2201 4005 4682 5830 6075 1606 Size: 595 1804 677 1148 245 2120 Fragments arranged by size: 2120 1804 1148 677 595 245 NaeI GCC'GGC Cuts at: 2257 2257 Size: 6589 NarI GG'CG_CC Cuts at: 2036 2036 Size: 6589 NcoI C'CATG_G Cuts at: 513 1065 2004 2391 513 Size: 552 939 387 4711 Fragments arranged by size: 4711 939 552 387 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NgoMIV G'CCGG_C Cuts at: 2255 2255 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NsiI A_TGCA'T Cuts at: 3752 3752 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3002 3947 5492 1132 Size: 97 1773 945 1545 2229 Fragments arranged by size: 2229 1773 1545 945 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6589 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PfoI T'CCnGG_A Cuts at: 3517 3517 Size: 6589 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 3621 4159 3621 Size: 538 6051 PspOMI G'GGCC_C Cuts at: 2227 2513 2227 Size: 286 6303 PstI C_TGCA'G Cuts at: 830 3941 830 Size: 3111 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 SacII CC_GC'GG Cuts at: 2202 2202 Size: 6589 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SexAI A'CCwGG_T Cuts at: 2052 2750 2052 Size: 698 5891 SfcI C'TryA_G Cuts at: 826 1045 3937 5072 5753 5944 826 Size: 219 2892 1135 681 191 1471 Fragments arranged by size: 2892 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2037 2037 Size: 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 2285 1700 Size: 585 6004 SmaI CCC'GGG Cuts at: 1872 3916 1872 Size: 2044 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2301 2913 3785 4693 5594 5856 Size: 197 926 358 612 872 908 901 262 Cuts at: 5856 820 Size: 1553 Fragments arranged by size: 1553 926 908 901 872 612 358 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 3196 4513 5 Size: 47 3144 1317 2081 Fragments arranged by size: 3144 2081 1317 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 3221 3681 4028 513 Size: 552 939 387 830 460 347 3074 Fragments arranged by size: 3074 939 830 552 460 387 347 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4616 4633 4786 4971 5416 325 Size: 1255 3036 17 153 185 445 1498 Fragments arranged by size: 3036 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3318 4835 6547 Size: 274 80 33 51 1443 1341 1517 1712 Cuts at: 6547 96 Size: 138 Fragments arranged by size: 1712 1517 1443 1341 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6549 6581 6549 Size: 32 6557 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2642 2642 Size: 6589 XhoI C'TCGA_G Cuts at: 1943 3785 1943 Size: 1842 4747 XmaI C'CCGG_G Cuts at: 1870 3914 1870 Size: 2044 4545 XmnI GAAnn'nnTTC Cuts at: 2849 3430 3510 4718 2849 Size: 581 80 1208 4720 Fragments arranged by size: 4720 1208 581 80 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI