(Linear) (Six-base) MAPSORT of: KIEE6784.seq Check: 4189 from: 1 to: 1884 KIEE6784.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AccI GT'mk_AC Cuts at: 0 635 1682 1884 Size: 635 1047 202 Fragments arranged by size: 1047 635 202 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1119 1504 1884 Size: 1119 385 380 Fragments arranged by size: 1119 385 380 AflIII A'CryG_T Cuts at: 0 606 1884 Size: 606 1278 AlwNI CAG_nnn'CTG Cuts at: 0 403 1884 Size: 403 1481 ApaI G_GGCC'C Cuts at: 0 226 512 1884 Size: 226 286 1372 Fragments arranged by size: 1372 286 226 ApaLI G'TGCA_C Cuts at: 0 590 1884 Size: 590 1294 AseI AT'TA_AT Cuts at: 0 1043 1884 Size: 1043 841 AvaI C'yCGr_G Cuts at: 0 78 338 495 1780 1884 Size: 78 260 157 1285 104 Fragments arranged by size: 1285 260 157 104 78 BanI G'GyrC_C Cuts at: 0 30 321 1110 1884 Size: 30 291 789 774 Fragments arranged by size: 789 774 291 30 BanII G_rGCy'C Cuts at: 0 128 226 512 1884 Size: 128 98 286 1372 Fragments arranged by size: 1372 286 128 98 BbeI G_GCGC'C Cuts at: 0 34 1884 Size: 34 1850 BbvCI CC'TCA_GC Cuts at: 0 130 1884 Size: 130 1754 BciVI GTATCCnnnnn_n' Cuts at: 0 283 1884 Size: 283 1601 BclI T'GATC_A Cuts at: 0 1056 1884 Size: 1056 828 BfrBI ATG'CAT Cuts at: 0 1745 1884 Size: 1745 139 Bme1580I G_kGCm'C Cuts at: 0 226 324 512 594 1115 1330 1884 Size: 226 98 188 82 521 215 554 Fragments arranged by size: 554 521 226 215 188 98 82 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 1884 Size: 724 1160 Bpu10I CC'TnA_GC Cuts at: 0 130 725 1170 1884 Size: 130 595 445 714 Fragments arranged by size: 714 595 445 130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 281 893 1884 Size: 281 612 991 Fragments arranged by size: 991 612 281 BsaAI yAC'GTr Cuts at: 0 609 1884 Size: 609 1275 BsaBI GATnn'nnATC Cuts at: 0 773 1884 Size: 773 1111 BsaHI Gr'CG_yC Cuts at: 0 31 1884 Size: 31 1853 BsaWI w'CCGG_w Cuts at: 0 1180 1884 Size: 1180 704 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 87 1884 Size: 87 1797 BseYI C'CCAG_C Cuts at: 0 41 116 1884 Size: 41 75 1768 Fragments arranged by size: 1768 75 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 261 1612 1884 Size: 261 1351 272 Fragments arranged by size: 1351 272 261 BsiEI CG_ry'CG Cuts at: 0 251 1884 Size: 251 1633 BsiHKAI G_wGCw'C Cuts at: 0 455 594 1592 1884 Size: 455 139 998 292 Fragments arranged by size: 998 455 292 139 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1777 1884 Size: 1777 107 Bsp1286I G_dGCh'C Cuts at: 0 128 226 324 455 512 594 1115 1330 Size: 128 98 98 131 57 82 521 215 Cuts at: 1330 1592 1884 Size: 262 292 Fragments arranged by size: 521 292 262 215 131 128 98 98 82 57 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 283 1884 Size: 283 1601 BsrBI CCG'CTC Cuts at: 0 83 1884 Size: 83 1801 BsrDI GCAATG_nn' Cuts at: 0 1745 1841 1884 Size: 1745 96 43 Fragments arranged by size: 1745 96 43 BsrFI r'CCGG_y Cuts at: 0 250 280 1884 Size: 250 30 1604 Fragments arranged by size: 1604 250 30 BssSI C'ACGA_G Cuts at: 0 1381 1884 Size: 1381 503 Bsu36I CC'TnA_GG Cuts at: 0 585 1884 Size: 585 1299 BtgI C'CryG_G Cuts at: 0 65 194 386 1884 Size: 65 129 192 1498 Fragments arranged by size: 1498 192 129 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1012 1884 Size: 1012 872 DraI TTT'AAA Cuts at: 0 1368 1884 Size: 1368 516 EaeI y'GGCC_r Cuts at: 0 107 248 525 627 867 1884 Size: 107 141 277 102 240 1017 Fragments arranged by size: 1017 277 240 141 107 102 EagI C'GGCC_G Cuts at: 0 248 1884 Size: 248 1636 EarI CTCTTCn'nnn_ Cuts at: 0 1496 1884 Size: 1496 388 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 724 1119 1504 1884 Size: 724 395 385 380 Fragments arranged by size: 724 395 385 380 EcoO109I rG'GnC_Cy Cuts at: 0 222 508 509 1884 Size: 222 286 1 1375 Fragments arranged by size: 1375 286 222 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1525 1884 Size: 1525 359 HaeII r_GCGC'y Cuts at: 0 34 1884 Size: 34 1850 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 79 111 1041 1073 1579 1611 Size: 46 32 1 32 930 32 506 32 Cuts at: 1611 1675 1707 1884 Size: 64 32 177 Fragments arranged by size: 930 506 177 64 46 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 0 162 650 690 1884 Size: 162 488 40 1194 Fragments arranged by size: 1194 488 162 40 KasI G'GCGC_C Cuts at: 0 30 1884 Size: 30 1854 MfeI C'AATT_G Cuts at: 0 1277 1301 1847 1884 Size: 1277 24 546 37 Fragments arranged by size: 1277 546 37 24 MscI TGG'CCA Cuts at: 0 527 629 869 1884 Size: 527 102 240 1015 Fragments arranged by size: 1015 527 240 102 MslI CAynn'nnrTG Cuts at: 0 611 1884 Size: 611 1273 MspA1I CmG'CkG Cuts at: 0 196 1884 Size: 196 1688 NaeI GCC'GGC Cuts at: 0 252 1884 Size: 252 1632 NarI GG'CG_CC Cuts at: 0 31 1884 Size: 31 1853 NcoI C'CATG_G Cuts at: 0 386 1884 Size: 386 1498 NgoMIV G'CCGG_C Cuts at: 0 250 1884 Size: 250 1634 NsiI A_TGCA'T Cuts at: 0 1747 1884 Size: 1747 137 NspI r_CATG'y Cuts at: 0 997 1884 Size: 997 887 PfoI T'CCnGG_A Cuts at: 0 1512 1884 Size: 1512 372 PsiI TTA'TAA Cuts at: 0 1616 1884 Size: 1616 268 PspOMI G'GGCC_C Cuts at: 0 222 508 1884 Size: 222 286 1376 Fragments arranged by size: 1376 286 222 SacII CC_GC'GG Cuts at: 0 197 1884 Size: 197 1687 SexAI A'CCwGG_T Cuts at: 0 47 745 1884 Size: 47 698 1139 Fragments arranged by size: 1139 698 47 SfoI GGC'GCC Cuts at: 0 32 1884 Size: 32 1852 SgrAI Cr'CCGG_yG Cuts at: 0 280 1884 Size: 280 1604 SmlI C'TyrA_G Cuts at: 0 296 908 1780 1884 Size: 296 612 872 104 Fragments arranged by size: 872 612 296 104 SspI AAT'ATT Cuts at: 0 1191 1884 Size: 1191 693 StyI C'CwwG_G Cuts at: 0 386 1216 1676 1884 Size: 386 830 460 208 Fragments arranged by size: 830 460 386 208 TatI w'GTAC_w Cuts at: 0 1313 1884 Size: 1313 571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 637 1884 Size: 637 1247 XhoI C'TCGA_G Cuts at: 0 1780 1884 Size: 1780 104 XmnI GAAnn'nnTTC Cuts at: 0 844 1425 1505 1884 Size: 844 581 80 379 Fragments arranged by size: 844 581 379 80 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI ApaLI AseI AvaI BanI BanII BbeI BbvCI BciVI BclI BfrBI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI Bsu36I BtgI BtgZI DraI EaeI EagI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MfeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PfoI PsiI PspOMI SacII SexAI SfoI SgrAI SmlI SspI StyI TatI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AsiSI AvrII BaeI BamHI BbsI BcgI BglI BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaXI BsiWI BsmI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I BtsI ClaI CspCI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MluI MmeI NdeI NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XmaI ZraI