Restriction map for
pFN21AE3167
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AvaI AviII BalI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BpuAI BsaAI BsaHI BsiEI BsiWI
BsmBI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MaeII MfeI
MluI MluNI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 328 GGTACC G^GTACC
AccII 985 CGCG CG^CG
AccIII 788 TCCGGA T^CCGGA
AlwNI 343 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 65 GGGCCC GGGCC^C
Asp700I 778 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 328 GGTACC G^GTACC
AspHI 655 GAGCTC G[AT]GC[AT]^C
AvrII 186 CCTAGG C^CTAGG
BamHI 602 GGATCC G^GATCC
BlnI 186 CCTAGG C^CTAGG
BpmI 211 CTGGAG CTGGAG(16/14)
BsaBI 549 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 991 GGTCTC GGTCTC(1/5)
BsaWI 788 TCCGGA [AT]^CCGG[AT]
BseAI 788 TCCGGA T^CCGGA
BsgI 466 GTGCAG GTGCAG(16/14)
BsiHKAI 655 GAGCTC G[AT]GC[AT]^C
BspEI 788 TCCGGA T^CCGGA
BsrFI 221 ACCGGC [GA]^CCGG[CT]
BssSI 43 CTCGTG CACGAG(-5/-1)
BstAPI 192 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 674 CATCC GGATG(2/0)
BstUI 985 CGCG CG^CG
BstXI 749 CCAGGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 221 ACCGGC [GA]^CCGG[CT]
DrdI 1045 GACCAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 278 CCATGG C^C[GA][CT]GG
EcoRI 23 GAATTC G^AATTC
EcoRV 553 GATATC GAT^ATC
FauI 851 GCGGG CCCGC(4/6)
FokI 674 CATCC GGATG(9/13)
KpnI 328 GGTACC GGTAC^C
MamI 549 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 788 TCCGGA T^CCGGA
MslI 276 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 985 CGCG CG^CG
NcoI 278 CCATGG C^CATGG
SacI 655 GAGCTC GAGCT^C
ScaI 978 AGTACT AGT^ACT
SmlI 652 CTTGAG C^T[CT][GA]AG
SpeI 387 ACTAGT A^CTAGT
StuI 316 AGGCCT AGG^CCT
XcmI 278 CCATGGTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 778 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 23 GAATTC [GA]^AATT[CT]
739 AAATTT
ApoI 23 GAATTC [GA]^AATT[CT]
739 AAATTT
AvaII 3 GGTCC G^G[AT]CC
1044 GGACC
BanI 245 GGCACC G^G[CT][GA]CC
328 GGTACC
BbvCI 109 GCTGAGG CCTCAGC(-5/-2)
820 GCTGAGG
BbvI 630 GCAGC GCAGC(8/12)
141 GCTGC
BcnI 179 CCCGG CC^[GC]GG
1058 CCCGG
BglI 67 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
100 GCCCTTCTGGC
BsmAI 971 GTCTC GTCTC(1/5)
992 GTCTC
BsmI 192 GCATTC GAATGC(1/-1)
725 GCATTC
Bsp1407I 1130 TGTACA T^GTACA
1165 TGTACA
BsrDI 684 CATTGC GCAATG(2/0)
927 CATTGC
BsrGI 1130 TGTACA T^GTACA
1165 TGTACA
BsrI 920 ACTGG ACTGG(1/-1)
867 CCAGT
BstYI 344 AGATCC [GA]^GATC[CT]
602 GGATCC
Cac8I 851 GCGGGC GC[ACGT]^[ACGT]GC
902 GCCAGC
DraII 64 AGGGCCC [GA]G^G[ACGT]CC[CT]
113 AGGCCCC
EarI 782 CTCTTC CTCTTC(1/4)
834 CTCTTC
EcoNI 129 CCTGATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
319 CCTCACAGAGG
EcoO109I 64 AGGGCCC [GA]G^G[ACGT]CC[CT]
113 AGGCCCC
Fnu4HI 141 GCTGC GC^[ACGT]GC
630 GCAGC
HphI 282 GGTGA GGTGA(8/7)
291 GGTGA
ItaI 141 GCTGC GC^[ACGT]GC
630 GCAGC
Ksp632I 782 CTCTTC CTCTTC(1/4)
834 CTCTTC
MaeIII 696 GTTAC ^GT[ACGT]AC
826 GTTAC
MflI 344 AGATCC [GA]^GATC[CT]
602 GGATCC
NciI 179 CCCGG CC^[GC]GG
1058 CCCGG
NspI 206 ACATGC [GA]CATG^[CT]
1168 ACATGC
SspBI 1130 TGTACA T^GTACA
1165 TGTACA
TseI 141 GCTGC G^C[AT]GC
630 GCAGC
XhoII 344 AGATCC [GA]^GATC[CT]
602 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BfaI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cfr13I DdeI DpnII EcoRII EcoT14I
HaeIII HapII HinfI HpaII MaeI
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI StyI TfiI Tsp509I TspRI