(Circular) (Six-base) MAPSORT of: pFN21AE3167.seq Check: 5465 from: 1 to: 5881 pFN21AE3167 5881 bp With 182 enzymes: SgfI * March 28, 2012 13:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 2333 3202 2333 Size: 869 5012 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3944 5328 5596 1632 Size: 2312 1384 268 1917 Fragments arranged by size: 2312 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 2285 1166 Size: 1119 4762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2353 3624 5196 2353 Size: 1271 1572 3038 Fragments arranged by size: 3038 1572 1271 ApaI G_GGCC'C Cuts at: 2074 2074 Size: 5881 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2028 2744 3190 3416 3568 5677 1493 Size: 535 716 446 226 152 2109 1697 Fragments arranged by size: 2109 1697 716 535 446 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 3206 1870 Size: 73 1263 4545 Fragments arranged by size: 4545 1263 73 AvrII C'CTAG_G Cuts at: 2191 2191 Size: 5881 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2317 2350 2317 Size: 33 5848 BamHI G'GATC_C Cuts at: 2607 3211 3578 2607 Size: 604 367 4910 Fragments arranged by size: 4910 604 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2333 3202 4657 618 Size: 325 217 627 463 83 869 1455 1842 Fragments arranged by size: 1842 1455 869 627 463 325 217 83 BanII G_rGCy'C Cuts at: 1391 2074 2466 2664 3200 1391 Size: 683 392 198 536 4072 Fragments arranged by size: 4072 683 536 392 198 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BbvCI CC'TCA_GC Cuts at: 2115 2826 2115 Size: 711 5170 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4072 4106 1903 Size: 34 2135 34 3678 Fragments arranged by size: 3678 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2111 4492 Size: 107 122 71 631 605 406 33 2381 Cuts at: 4492 136 Size: 1525 Fragments arranged by size: 2381 1525 631 605 406 122 107 71 33 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 2074 2253 3942 5098 1790 Size: 284 179 1689 1156 2573 Fragments arranged by size: 2573 1689 1156 284 179 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3612 4570 442 Size: 416 53 83 399 24 2195 958 1753 Fragments arranged by size: 2195 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 4541 1239 Size: 998 2304 2579 Fragments arranged by size: 2579 2304 998 Bpu10I CC'TnA_GC Cuts at: 2115 2826 2115 Size: 711 5170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2678 4006 4871 5169 2678 Size: 1328 865 298 3390 Fragments arranged by size: 3390 1328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3002 4544 882 Size: 617 1503 1542 2219 Fragments arranged by size: 2219 1542 1503 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 2558 5875 2558 Size: 3317 2564 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2793 4314 4712 4986 5133 5544 2793 Size: 1521 398 274 147 411 3130 Fragments arranged by size: 3130 1521 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5656 Fragments arranged by size: 5656 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2003 2156 2780 1186 Size: 817 153 624 4287 Fragments arranged by size: 4287 817 624 153 BseYI C'CCAG_C Cuts at: 2911 5084 5580 2911 Size: 2173 496 3212 Fragments arranged by size: 3212 2173 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2492 1163 Size: 573 756 4552 Fragments arranged by size: 4552 756 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2664 3942 4027 5098 2664 Size: 1278 85 1071 3447 Fragments arranged by size: 3447 1278 1071 85 BsmI GAATG_Cn' Cuts at: 2197 2730 3392 3485 2197 Size: 533 662 93 4593 Fragments arranged by size: 4593 662 533 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2253 2466 2664 3200 3942 4027 Size: 399 284 179 213 198 536 742 85 Cuts at: 4027 5098 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 284 213 198 179 85 BspEI T'CCGG_A Cuts at: 2793 5544 2793 Size: 2751 3130 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3236 844 Size: 2392 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2688 2931 4376 4550 65 Size: 1183 1440 243 1445 174 1396 Fragments arranged by size: 1445 1440 1396 1183 243 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 4525 4712 1700 Size: 110 416 2299 187 2869 Fragments arranged by size: 2869 2299 416 187 110 BsrGI T'GTAC_A Cuts at: 96 3135 3170 96 Size: 3039 35 2807 Fragments arranged by size: 3039 2807 35 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 2048 3941 4953 2048 Size: 1893 1012 2976 Fragments arranged by size: 2976 1893 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2203 794 Size: 1409 4472 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstXI CCAn_nnnn'nTGG Cuts at: 2761 2761 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2349 2607 3211 3578 3963 3980 5432 5518 Size: 377 258 604 367 385 17 1452 86 Cuts at: 5518 5530 5876 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 604 385 377 367 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2283 513 Size: 552 939 279 4111 Fragments arranged by size: 4111 939 552 279 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3006 487 Size: 683 56 1780 3362 Fragments arranged by size: 3362 1780 683 56 BtsI GCAGTG_nn' Cuts at: 2201 3397 4191 4211 2201 Size: 1196 794 20 3871 Fragments arranged by size: 3871 1196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3056 4888 809 Size: 507 1740 1832 1802 Fragments arranged by size: 1832 1802 1740 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846 Size: 54 1425 50 275 18 1498 889 1629 Cuts at: 5846 8 Size: 43 Fragments arranged by size: 1629 1498 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 2845 3811 1353 Size: 1440 52 966 3423 Fragments arranged by size: 3423 1440 966 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2894 4468 4842 4988 387 Size: 1064 138 1305 1574 374 146 1280 Fragments arranged by size: 1574 1305 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2662 2662 Size: 5881 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 3944 4541 5328 5596 1239 Size: 393 605 1707 597 787 268 1524 Fragments arranged by size: 1707 1524 787 605 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2328 2138 Size: 190 5691 EcoO109I rG'GnC_Cy Cuts at: 2070 2119 2070 Size: 49 5832 EcoRI G'AATT_C Cuts at: 2028 3190 3568 2028 Size: 1162 378 4341 Fragments arranged by size: 4341 1162 378 EcoRV GAT'ATC Cuts at: 2560 2560 Size: 5881 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2592 2624 2804 2836 2920 Size: 32 135 32 1020 32 180 32 84 Cuts at: 2920 2952 4522 4554 4596 4628 1373 Size: 32 1570 32 42 32 2626 Fragments arranged by size: 2626 1570 1020 180 135 84 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 2337 3206 2337 Size: 869 5012 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2452 4995 5179 1305 Size: 1147 2543 184 2007 Fragments arranged by size: 2543 2007 1147 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 3839 4198 4357 518 Size: 648 1119 1554 359 159 2042 Fragments arranged by size: 2042 1554 1119 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2237 2825 2911 3297 3974 5122 5367 1606 Size: 631 588 86 386 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 631 588 386 245 86 NcoI C'CATG_G Cuts at: 513 1065 2004 2283 513 Size: 552 939 279 4111 Fragments arranged by size: 4111 939 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2215 3177 3239 4784 1132 Size: 97 986 962 62 1545 2229 Fragments arranged by size: 2229 1545 986 962 97 62 PasI CC'CwG_GG Cuts at: 1348 2466 2754 1348 Size: 1118 288 4475 Fragments arranged by size: 4475 1118 288 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PspOMI G'GGCC_C Cuts at: 2070 2070 Size: 5881 PstI C_TGCA'G Cuts at: 830 3233 830 Size: 2403 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2237 2825 2911 2237 Size: 588 86 5207 Fragments arranged by size: 5207 588 86 SacI G_AGCT'C Cuts at: 2664 2664 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 2985 4129 5841 2985 Size: 1144 1712 3025 Fragments arranged by size: 3025 1712 1144 SfcI C'TryA_G Cuts at: 826 1045 3229 4364 5045 5236 826 Size: 219 2184 1135 681 191 1471 Fragments arranged by size: 2184 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 3985 4886 5148 820 Size: 197 926 714 1328 901 262 1553 Fragments arranged by size: 1553 1328 926 901 714 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 2392 152 Size: 2240 3641 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 2323 1517 Size: 806 5075 StyI C'CwwG_G Cuts at: 513 1065 2004 2174 2191 2283 2682 3320 513 Size: 552 939 170 17 92 399 638 3074 Fragments arranged by size: 3074 939 638 552 399 170 92 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2458 3908 3925 4078 4263 4708 325 Size: 1255 878 1450 17 153 185 445 1498 Fragments arranged by size: 1498 1450 1255 878 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2983 3135 3170 Size: 274 80 33 51 1443 1006 152 35 Cuts at: 3170 4127 5839 96 Size: 957 1712 138 Fragments arranged by size: 1712 1443 1006 957 274 152 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2483 2515 5841 5873 2483 Size: 32 3326 32 2491 Fragments arranged by size: 3326 2491 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2290 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 2787 4010 2787 Size: 1223 4658 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI