Restriction map for
pFN21AE6279
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI KpnI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI Sau3AI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AfaI 108 GTAC GT^AC
ApaI 479 GGGCCC GGGCC^C
AspHI 226 GTGCTC G[AT]GC[AT]^C
BbsI 529 GTCTTC GAAGAC(2/6)
BbvCI 153 CCTCAGC CCTCAGC(-5/-2)
BfaI 118 CTAG C^TAG
BpmI 382 CTCCAG CTGGAG(16/14)
BpuAI 529 GTCTTC GAAGAC(2/6)
BseRI 687 CTCCTC GAGGAG(10/8)
BsiHKAI 226 GTGCTC G[AT]GC[AT]^C
BsmI 138 GAATGC GAATGC(1/-1)
BspMI 241 ACCTGC ACCTGC(4/8)
BsrDI 602 GCAATG GCAATG(2/0)
BstXI 203 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 154 CTCAG C^T[ACGT]AG
EcoNI 300 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 118 CTAG C^TAG
MslI 204 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 656 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 658 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 652 CTGCAG CTGCA^G
RsaI 108 GTAC GT^AC
SapI 255 GCTCTTC GCTCTTC(1/4)
SexAI 287 ACCTGGT A^CC[AT]GGT
SfcI 652 CTGCAG C^T[GA][CT]AG
SmlI 337 CTCAAG C^T[CT][GA]AG
Tsp45I 722 GTCAC ^GT[GC]AC
XcmI 200 CCACCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccII 30 CGCG CG^CG
57 CGCG
AcyI 33 GGCGCC G[GA]^CG[CT]C
98 GGCGCC
BanI 33 GGCGCC G^G[CT][GA]CC
98 GGCGCC
BanII 479 GGGCCC G[GA]GC[CT]^C
557 GAGCCC
BbeI 33 GGCGCC GGCGC^C
98 GGCGCC
BglI 82 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
499 GCCTCCCGGGC
BsaHI 33 GGCGCC G[GA]^CG[CT]C
98 GGCGCC
BsaI 543 GAGACC GGTCTC(1/5)
235 GGTCTC
BsgI 319 GTGCAG GTGCAG(16/14)
438 GTGCAG
BsmAI 543 GAGAC GTCTC(1/5)
236 GTCTC
BstF5I 150 CATCC GGATG(2/0)
159 CATCC
BstUI 30 CGCG CG^CG
57 CGCG
CfoI 34 GCGC GCG^C
99 GCGC
FauI 170 CCCGC CCCGC(4/6)
58 GCGGG
FokI 150 CATCC GGATG(9/13)
159 CATCC
HaeII 33 GGCGCC [GA]GCGC^[CT]
98 GGCGCC
HhaI 34 GCGC GCG^C
99 GCGC
Hin1I 33 GGCGCC G[GA]^CG[CT]C
98 GGCGCC
HinP1I 34 GCGC G^CGC
99 GCGC
KasI 33 GGCGCC G^GCGCC
98 GGCGCC
KspI 29 CCGCGG CCGC^GG
56 CCGCGG
MaeII 175 ACGT A^CGT
278 ACGT
MaeIII 275 GTAAC ^GT[ACGT]AC
722 GTCAC
MvnI 30 CGCG CG^CG
57 CGCG
NarI 33 GGCGCC GG^CGCC
98 GGCGCC
NlaIII 216 CATG CATG^
304 CATG
PvuII 410 CAGCTG CAG^CTG
524 CAGCTG
SacII 29 CCGCGG CCGC^GG
56 CCGCGG
SfaNI 158 GCATC GCATC(5/9)
648 GCATC
SfoI 33 GGCGCC GGC^GCC
98 GGCGCC
SmaI 37 CCCGGG CCC^GGG
503 CCCGGG
XmaCI 37 CCCGGG C^CCGGG
503 CCCGGG
XmaI 37 CCCGGG C^CCGGG
503 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DraII DsaI
EarI EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI Ksp632I
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI
TseI TspRI