(Circular) (Six-base) MAPSORT of: pFN21AE6279.seq Check: 8514 from: 1 to: 5431 pFN21AE6279 5431 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2255 2255 Size: 5431 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 3494 4878 5146 1632 Size: 497 1365 1384 268 1917 Fragments arranged by size: 1917 1384 1365 497 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 4330 5307 1203 Size: 3127 977 1327 Fragments arranged by size: 3127 1327 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 2213 1166 Size: 1047 4384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 3174 4746 3174 Size: 1572 3859 ApaI G_GGCC'C Cuts at: 2488 2488 Size: 5431 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2740 2966 3118 5227 1493 Size: 1247 226 152 2109 1697 Fragments arranged by size: 2109 1697 1247 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2437 2508 2756 1870 Size: 73 99 395 71 248 4545 Fragments arranged by size: 4545 395 248 99 73 71 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2103 2752 4207 618 Size: 325 217 627 251 65 649 1455 1842 Fragments arranged by size: 1842 1455 649 627 325 251 217 65 BanII G_rGCy'C Cuts at: 1391 2488 2566 2750 1391 Size: 1097 78 184 4072 Fragments arranged by size: 4072 1097 184 78 BbeI G_GCGC'C Cuts at: 2042 2107 2042 Size: 65 5366 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2527 928 Size: 971 628 3832 Fragments arranged by size: 3832 971 628 BbvCI CC'TCA_GC Cuts at: 2159 2159 Size: 5431 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3622 3656 1903 Size: 34 1685 34 3678 Fragments arranged by size: 3678 1685 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2510 4042 Size: 107 122 71 631 605 421 417 1532 Cuts at: 4042 136 Size: 1525 Fragments arranged by size: 1532 1525 631 605 421 417 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2848 2848 Size: 5431 Bme1580I G_kGCm'C Cuts at: 1790 2358 2488 3492 4648 1790 Size: 568 130 1004 1156 2573 Fragments arranged by size: 2573 1156 1004 568 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2643 3162 4120 Size: 416 53 83 399 24 1226 519 958 Cuts at: 4120 442 Size: 1753 Fragments arranged by size: 1753 1226 958 519 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 4091 1239 Size: 1133 1719 2579 Fragments arranged by size: 2579 1719 1133 Bpu10I CC'TnA_GC Cuts at: 2159 2159 Size: 5431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 3556 4421 4719 2327 Size: 1229 865 298 3039 Fragments arranged by size: 3039 1229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2246 2542 4094 882 Size: 617 747 296 1552 2219 Fragments arranged by size: 2219 1552 747 617 296 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 2104 3238 3620 Size: 53 83 186 936 506 65 1134 382 Cuts at: 3620 4255 275 Size: 635 1451 Fragments arranged by size: 1451 1134 936 635 506 382 186 83 65 53 BsaWI w'CCGG_w Cuts at: 3864 4262 4536 4683 5094 3864 Size: 398 274 147 411 4201 Fragments arranged by size: 4201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5206 Fragments arranged by size: 5206 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2683 1186 Size: 1497 3934 BseYI C'CCAG_C Cuts at: 4634 5130 4634 Size: 496 4935 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2464 1163 Size: 573 609 119 4130 Fragments arranged by size: 4130 609 573 119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3642 4670 1540 Size: 275 186 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2235 3492 3577 4648 2235 Size: 1257 85 1071 3018 Fragments arranged by size: 3018 1257 1071 85 BsmI GAATG_Cn' Cuts at: 2149 2942 3035 2149 Size: 793 93 4545 Fragments arranged by size: 4545 793 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2358 2488 2566 2750 3492 3577 Size: 399 445 123 130 78 184 742 85 Cuts at: 3577 4648 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 445 399 184 130 123 85 78 BspEI T'CCGG_A Cuts at: 5094 5094 Size: 5431 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2255 2786 844 Size: 1411 531 3489 Fragments arranged by size: 3489 1411 531 BsrBI CCG'CTC Cuts at: 1394 1823 3318 1394 Size: 429 1495 3507 Fragments arranged by size: 3507 1495 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 3926 4100 65 Size: 1183 1366 1312 174 1396 Fragments arranged by size: 1396 1366 1312 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4075 4262 1700 Size: 110 2265 187 2869 Fragments arranged by size: 2869 2265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5431 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2215 Size: 5431 BstYI r'GATC_y Cuts at: 1972 2761 3128 3513 3530 4982 5068 5080 5426 Size: 789 367 385 17 1452 86 12 346 Cuts at: 5426 1972 Size: 1977 Fragments arranged by size: 1977 1452 789 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2061 2543 513 Size: 552 939 30 27 482 3401 Fragments arranged by size: 3401 939 552 482 30 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2208 2358 2947 3741 3761 2208 Size: 150 589 794 20 3878 Fragments arranged by size: 3878 794 589 150 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396 Size: 54 1425 50 275 18 1048 889 1629 Cuts at: 5396 8 Size: 43 Fragments arranged by size: 1629 1425 1048 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 2306 3361 1353 Size: 914 39 1055 3423 Fragments arranged by size: 3423 1055 914 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2240 2465 4018 4392 4538 387 Size: 1064 138 651 225 1553 374 146 1280 Fragments arranged by size: 1553 1280 1064 651 374 225 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2372 3494 4091 4878 5146 1239 Size: 393 497 243 1122 597 787 268 1524 Fragments arranged by size: 1524 1122 787 597 497 393 268 243 EcoNI CCTnn'n_nnAGG Cuts at: 2309 2309 Size: 5431 EcoO109I rG'GnC_Cy Cuts at: 2485 2662 2684 2485 Size: 177 22 5232 Fragments arranged by size: 5232 177 22 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2042 2107 4578 1678 Size: 364 65 2471 2531 Fragments arranged by size: 2531 2471 364 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2539 2571 4072 4104 4146 Size: 32 135 32 967 32 1501 32 42 Cuts at: 4146 4178 1373 Size: 32 2626 Fragments arranged by size: 2626 1501 967 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2038 2103 2038 Size: 65 5366 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4545 4729 1305 Size: 3240 184 2007 Fragments arranged by size: 3240 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 3389 3748 3907 518 Size: 648 1047 1176 359 159 2042 Fragments arranged by size: 2042 1176 1047 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2036 2063 2417 2531 2847 3524 4672 Size: 406 24 27 354 114 316 677 1148 Cuts at: 4672 4917 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 406 354 316 245 114 27 24 NarI GG'CG_CC Cuts at: 2039 2104 2039 Size: 65 5366 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3940 Fragments arranged by size: 3940 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2789 4334 1132 Size: 97 1560 1545 2229 Fragments arranged by size: 2229 1560 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2662 2662 Size: 5431 PshAI GACnn'nnGTC Cuts at: 1613 2667 1613 Size: 1054 4377 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PspOMI G'GGCC_C Cuts at: 2484 2484 Size: 5431 PstI C_TGCA'G Cuts at: 830 2661 2783 830 Size: 1831 122 3478 Fragments arranged by size: 3478 1831 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 PvuII CAG'CTG Cuts at: 2417 2531 2417 Size: 114 5317 SacII CC_GC'GG Cuts at: 2037 2064 2037 Size: 27 5404 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 5431 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SexAI A'CCwGG_T Cuts at: 2292 2292 Size: 5431 SfcI C'TryA_G Cuts at: 826 1045 2657 2779 3914 4595 4786 826 Size: 219 1612 122 1135 681 191 1471 Fragments arranged by size: 1612 1471 1135 681 219 191 122 SfoI GGC'GCC Cuts at: 2040 2105 2040 Size: 65 5366 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2044 2510 2758 1872 Size: 172 466 248 4545 Fragments arranged by size: 4545 466 248 172 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 3535 4436 4698 820 Size: 197 926 399 1193 901 262 1553 Fragments arranged by size: 1553 1193 926 901 399 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SrfI GCCC'GGGC Cuts at: 2044 2044 Size: 5431 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2004 2870 513 Size: 552 939 866 3074 Fragments arranged by size: 3074 939 866 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3458 3475 3628 3813 4258 325 Size: 1255 1878 17 153 185 445 1498 Fragments arranged by size: 1878 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3677 5389 Size: 274 80 33 51 1443 135 1565 1712 Cuts at: 5389 96 Size: 138 Fragments arranged by size: 1712 1565 1443 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2074 2106 5391 5423 2074 Size: 32 3285 32 2082 Fragments arranged by size: 3285 2082 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2212 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2042 2508 2756 1870 Size: 172 466 248 4545 Fragments arranged by size: 4545 466 248 172 XmnI GAAnn'nnTTC Cuts at: 3560 3560 Size: 5431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SfiI SwaI