Restriction map for
pFN21AE6190
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 128 GAATTC [GA]^AATT[CT]
AgeI 137 ACCGGT A^CCGGT
AocI 466 CCTAAGG CC^T[ACGT]AGG
ApoI 128 GAATTC [GA]^AATT[CT]
AvaI 14 CTCGGG C^[CT]CG[GA]G
AvaII 447 GGACC G^G[AT]CC
BalI 329 TGGCCA TGG^CCA
BbsI 88 GTCTTC GAAGAC(2/6)
BlpI 341 GCTCAGC GC^T[ACGT]AGC
BpmI 442 CTGGAG CTGGAG(16/14)
Bpu1102I 341 GCTCAGC GC^T[ACGT]AGC
BpuAI 88 GTCTTC GAAGAC(2/6)
BsaWI 137 ACCGGT [AT]^CCGG[AT]
BsoBI 14 CTCGGG C^[CT]CG[GA]G
BsrFI 137 ACCGGT [GA]^CCGG[CT]
Bsu36I 466 CCTAAGG CC^T[ACGT]AGG
CelII 341 GCTCAGC GC^T[ACGT]AGC
CfoI 430 GCGC GCG^C
Cfr10I 137 ACCGGT [GA]^CCGG[CT]
EaeI 329 TGGCCA [CT]^GGCC[GA]
Eco81I 466 CCTAAGG CC^T[ACGT]AGG
EcoNI 372 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 128 GAATTC G^AATTC
FauI 314 CCCGC CCCGC(4/6)
HhaI 430 GCGC GCG^C
HinP1I 430 GCGC G^CGC
HinfI 86 GAGTC G^A[ACGT]TC
HphI 278 TCACC GGTGA(8/7)
MboII 89 TCTTC GAAGA(8/7)
MluNI 329 TGGCCA TGG^CCA
MscI 329 TGGCCA TGG^CCA
MspA1I 457 CAGCTG C[AC]G^C[GT]G
PinAI 137 ACCGGT A^CCGGT
PleI 86 GAGTC GAGTC(4/5)
PpuMI 446 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 457 CAGCTG CAG^CTG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 9 GTAC GT^AC
510 GTAC
BamHI 167 GGATCC G^GATCC
261 GGATCC
BanI 134 GGCACC G^G[CT][GA]CC
475 GGCACC
BbvI 310 GCTGC GCAGC(8/12)
459 GCTGC
BcnI 296 CCGGG CC^[GC]GG
480 CCCGG
BseRI 394 CTCCTC GAGGAG(10/8)
4 GAGGAG
BsrDI 113 GCAATG GCAATG(2/0)
346 GCAATG
BsrI 327 ACTGG ACTGG(1/-1)
281 CCAGT
BstYI 167 GGATCC [GA]^GATC[CT]
261 GGATCC
DdeI 342 CTCAG C^T[ACGT]AG
467 CTAAG
DpnII 168 GATC ^GATC
262 GATC
DraII 209 GGGGCCT [GA]G^G[ACGT]CC[CT]
446 AGGACCC
DsaI 28 CCATGG C^C[GA][CT]GG
332 CCATGG
EcoO109I 209 GGGGCCT [GA]G^G[ACGT]CC[CT]
446 AGGACCC
EcoT14I 28 CCATGG C^C[AT][AT]GG
332 CCATGG
MboI 168 GATC ^GATC
262 GATC
MflI 167 GGATCC [GA]^GATC[CT]
261 GGATCC
MwoI 301 GCTGTGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
491 GCTACCCCAGC
NciI 296 CCGGG CC^[GC]GG
480 CCCGG
NcoI 28 CCATGG C^CATGG
332 CCATGG
NdeII 168 GATC ^GATC
262 GATC
NlaIII 29 CATG CATG^
333 CATG
RsaI 9 GTAC GT^AC
510 GTAC
Sau3AI 168 GATC ^GATC
262 GATC
SfaNI 359 GCATC GCATC(5/9)
389 GCATC
StyI 28 CCATGG C^C[AT][AT]GG
332 CCATGG
TseI 310 GCTGC G^C[AT]GC
459 GCTGC
Tsp509I 36 AATT ^AATT
129 AATT
XhoII 167 GGATCC [GA]^GATC[CT]
261 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AlwI BanII BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MnlI MspI MvaI NlaIV
Sau96I ScrFI TspRI