(Circular) (Six-base) MAPSORT of: pFN21AE6190.seq Check: 5435 from: 1 to: 5221 pFN21AE6190 5221 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3031 278 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2564 1937 Size: 627 4594 Acc65I G'GTAC_C Cuts at: 2542 2542 Size: 5221 AclI AA'CG_TT Cuts at: 1563 3348 3721 1563 Size: 1785 373 3063 Fragments arranged by size: 3063 1785 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3284 4668 4936 1632 Size: 1652 1384 268 1917 Fragments arranged by size: 1917 1652 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5024 AflIII A'CryG_T Cuts at: 1203 4120 5097 1203 Size: 2917 977 1327 Fragments arranged by size: 2917 1327 977 AgeI A'CCGG_T Cuts at: 2142 4052 2142 Size: 1910 3311 AhdI GACnn_n'nnGTC Cuts at: 3950 3950 Size: 5221 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5221 AlwNI CAG_nnn'CTG Cuts at: 2964 4536 2964 Size: 1572 3649 ApaLI G'TGCA_C Cuts at: 3278 4434 3278 Size: 1156 4065 ApoI r'AATT_y Cuts at: 1493 2133 2530 2756 2908 5017 1493 Size: 640 397 226 152 2109 1697 Fragments arranged by size: 2109 1697 640 397 226 152 AseI AT'TA_AT Cuts at: 160 1032 3775 160 Size: 872 2743 1606 Fragments arranged by size: 2743 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2546 1870 Size: 73 76 527 4545 Fragments arranged by size: 4545 527 76 73 BamHI G'GATC_C Cuts at: 2172 2266 2551 2918 2172 Size: 94 285 367 4475 Fragments arranged by size: 4475 367 285 94 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2139 2480 2542 3997 618 Size: 325 217 627 352 341 62 1455 1842 Fragments arranged by size: 1842 1455 627 352 341 325 217 62 BanII G_rGCy'C Cuts at: 1391 2028 2182 2348 2540 1391 Size: 637 154 166 192 4072 Fragments arranged by size: 4072 637 192 166 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 928 Size: 971 187 4063 Fragments arranged by size: 4063 971 187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3412 3446 1903 Size: 34 1475 34 3678 Fragments arranged by size: 3678 1475 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3113 4323 3113 Size: 1210 4011 BclI T'GATC_A Cuts at: 1548 1548 Size: 5221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3832 136 Size: 107 122 71 631 605 2160 1525 Fragments arranged by size: 2160 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5216 5216 Size: 5221 BlpI GC'TnA_GC Cuts at: 2347 2638 2347 Size: 291 4930 Bme1580I G_kGCm'C Cuts at: 1790 3282 4438 1790 Size: 1492 1156 2573 Fragments arranged by size: 2573 1492 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2952 3910 442 Size: 416 53 83 399 24 1535 958 1753 Fragments arranged by size: 1753 1535 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 3881 1239 Size: 1229 1413 2579 Fragments arranged by size: 2579 1413 1229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3346 4211 4509 3346 Size: 865 298 4058 Fragments arranged by size: 4058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3884 882 Size: 617 2385 2219 Fragments arranged by size: 2385 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3012 493 Size: 711 1808 2702 Fragments arranged by size: 2702 1808 711 BsaBI GATnn'nnATC Cuts at: 5215 5215 Size: 5221 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3028 3410 4045 275 Size: 53 83 186 936 1495 382 635 1451 Fragments arranged by size: 1495 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 3654 4052 4326 4473 4884 2142 Size: 1512 398 274 147 411 2479 Fragments arranged by size: 2479 1512 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4996 Fragments arranged by size: 4996 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2390 1186 Size: 838 366 4017 Fragments arranged by size: 4017 838 366 BseYI C'CCAG_C Cuts at: 2418 2456 2501 4424 4920 2418 Size: 38 45 1923 496 2719 Fragments arranged by size: 2719 1923 496 45 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4648 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2671 3432 4460 1540 Size: 275 186 670 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 275 186 BsiHKAI G_wGCw'C Cuts at: 3282 3367 4438 3282 Size: 85 1071 4065 Fragments arranged by size: 4065 1071 85 BsmI GAATG_Cn' Cuts at: 2732 2825 2732 Size: 93 5128 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2182 2348 2540 3282 3367 4438 Size: 399 238 154 166 192 742 85 1071 Cuts at: 4438 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 238 192 166 154 85 BspEI T'CCGG_A Cuts at: 4884 4884 Size: 5221 BspHI T'CATG_A Cuts at: 3110 4840 3110 Size: 1730 3491 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2576 844 Size: 1732 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3108 1394 Size: 429 1285 3507 Fragments arranged by size: 3507 1285 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2125 2358 3716 3890 65 Size: 1183 877 233 1358 174 1396 Fragments arranged by size: 1396 1358 1183 877 233 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2142 3865 4052 1700 Size: 110 332 1723 187 2869 Fragments arranged by size: 2869 1723 332 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5221 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5221 BssSI C'ACGA_G Cuts at: 3281 4293 3281 Size: 1012 4209 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5221 BstBI TT'CG_AA Cuts at: 4025 4025 Size: 5221 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5221 BstYI r'GATC_y Cuts at: 1972 2172 2266 2551 2918 3303 3320 4772 4858 Size: 200 94 285 367 385 17 1452 86 Cuts at: 4858 4870 5216 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 285 200 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2472 2472 Size: 5221 BtgI C'CryG_G Cuts at: 513 1065 2004 2033 2337 513 Size: 552 939 29 304 3397 Fragments arranged by size: 3397 939 552 304 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4482 Fragments arranged by size: 4482 683 56 BtsI GCAGTG_nn' Cuts at: 2211 2329 2737 3531 3551 2211 Size: 118 408 794 20 3881 Fragments arranged by size: 3881 794 408 118 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5221 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5186 DraI TTT'AAA Cuts at: 2525 2525 Size: 5221 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4228 809 Size: 507 2912 1802 Fragments arranged by size: 2912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2668 3557 Size: 54 1425 50 275 18 504 334 889 Cuts at: 3557 5186 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 504 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2668 1537 Size: 275 856 4090 Fragments arranged by size: 4090 856 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3151 1353 Size: 1798 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2103 3808 4182 4328 387 Size: 1064 138 514 1705 374 146 1280 Fragments arranged by size: 1705 1280 1064 514 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 3284 3881 4668 4936 1239 Size: 393 836 816 597 787 268 1524 Fragments arranged by size: 1524 836 816 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2381 2381 Size: 5221 EcoO109I rG'GnC_Cy Cuts at: 2215 2452 2215 Size: 237 4984 EcoRI G'AATT_C Cuts at: 2133 2530 2908 2133 Size: 397 378 4446 Fragments arranged by size: 4446 397 378 FspI TGC'GCA Cuts at: 3727 3727 Size: 5221 HaeII r_GCGC'y Cuts at: 1678 4368 1678 Size: 2690 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3862 3894 3936 3968 1373 Size: 32 135 32 2290 32 42 32 2626 Fragments arranged by size: 2626 2290 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2565 2811 669 Size: 988 281 627 246 3079 Fragments arranged by size: 3079 988 627 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5221 HpaI GTT'AAC Cuts at: 2811 2811 Size: 5221 KpnI G_GTAC'C Cuts at: 2546 2546 Size: 5221 MfeI C'AATT_G Cuts at: 2820 2820 Size: 5221 MluI A'CGCG_T Cuts at: 5097 5097 Size: 5221 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 4335 4519 1305 Size: 805 2225 184 2007 Fragments arranged by size: 2225 2007 805 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2336 10 Size: 54 1425 343 504 2895 Fragments arranged by size: 2895 1425 504 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3179 3538 3697 518 Size: 648 2013 359 159 2042 Fragments arranged by size: 2042 2013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2464 2637 3314 4462 4707 1606 Size: 858 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 858 677 245 173 NcoI C'CATG_G Cuts at: 513 1065 2004 2033 2337 513 Size: 552 939 29 304 3397 Fragments arranged by size: 3397 939 552 304 29 NdeI CA'TA_TG Cuts at: 387 387 Size: 5221 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5221 NotI GC'GGCC_GC Cuts at: 2668 2668 Size: 5221 NspI r_CATG'y Cuts at: 1132 1229 2579 4124 1132 Size: 97 1350 1545 2229 Fragments arranged by size: 2229 1545 1350 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5221 PciI A'CATG_T Cuts at: 4120 4120 Size: 5221 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5221 PfoI T'CCnGG_A Cuts at: 2445 2445 Size: 5221 PmeI GTTT'AAAC Cuts at: 2525 2525 Size: 5221 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3290 3322 4803 4835 3290 Size: 32 1481 32 3676 Fragments arranged by size: 3676 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2452 Size: 5221 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5221 PsiI TTA'TAA Cuts at: 2791 2791 Size: 5221 PstI C_TGCA'G Cuts at: 830 2573 830 Size: 1743 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5221 PvuII CAG'CTG Cuts at: 2464 2464 Size: 5221 SalI G'TCGA_C Cuts at: 1936 2563 1936 Size: 627 4594 SbfI CC_TGCA'GG Cuts at: 2573 2573 Size: 5221 ScaI AGT'ACT Cuts at: 3469 5181 3469 Size: 1712 3509 SfcI C'TryA_G Cuts at: 826 1045 2569 3704 4385 4576 826 Size: 219 1524 1135 681 191 1471 Fragments arranged by size: 1524 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5221 SmaI CCC'GGG Cuts at: 1872 2548 1872 Size: 676 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3325 4226 4488 820 Size: 197 926 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3012 493 Size: 2519 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5221 SphI G_CATG'C Cuts at: 1132 2579 1132 Size: 1447 3774 SspI AAT'ATT Cuts at: 5 52 3145 5 Size: 47 3093 2081 Fragments arranged by size: 3093 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5221 StyI C'CwwG_G Cuts at: 513 1065 2004 2033 2337 2660 513 Size: 552 939 29 304 323 3074 Fragments arranged by size: 3074 939 552 323 304 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 3248 3265 3418 3603 4048 325 Size: 1255 483 1185 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1185 483 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3467 5179 96 Size: 274 80 33 51 1443 1490 1712 138 Fragments arranged by size: 1712 1490 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5181 5213 5181 Size: 32 5189 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5221 XbaI T'CTAG_A Cuts at: 2557 2557 Size: 5221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5221 XmaI C'CCGG_G Cuts at: 1870 2546 1870 Size: 676 4545 XmnI GAAnn'nnTTC Cuts at: 3350 3350 Size: 5221 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3029 276 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI