################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PleI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 9 GACGTC GACGT^C AccI 31 GTCTAC GT^[AC][GT]AC AcsI 103 GAATTC [GA]^AATT[CT] AcyI 9 GACGTC G[GA]^CG[CT]C AgeI 364 ACCGGT A^CCGGT ApoI 103 GAATTC [GA]^AATT[CT] AvaI 604 CCCGAG C^[CT]CG[GA]G BbrPI 208 CACGTG CAC^GTG BlpI 174 GCTGAGC GC^T[ACGT]AGC Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC BsaAI 208 CACGTG [CT]AC^GT[GA] BsaHI 9 GACGTC G[GA]^CG[CT]C BsaI 367 GGTCTC GGTCTC(1/5) BsaWI 364 ACCGGT [AT]^CCGG[AT] BsmAI 368 GTCTC GTCTC(1/5) BsoBI 604 CCCGAG C^[CT]CG[GA]G BspLU11I 432 ACATGT A^CATGT BsrDI 423 CATTGC GCAATG(2/0) BsrFI 364 ACCGGT [GA]^CCGG[CT] CelII 174 GCTGAGC GC^T[ACGT]AGC CfoI 74 GCGC GCG^C Cfr10I 364 ACCGGT [GA]^CCGG[CT] CpoI 737 CGGACCG CG^G[AT]CCG DraII 716 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 208 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 42 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 716 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 103 GAATTC G^AATTC HaeII 73 AGCGCC [GA]GCGC^[CT] HgaI 489 GACGC GACGC(5/10) HhaI 74 GCGC GCG^C Hin1I 9 GACGTC G[GA]^CG[CT]C HinP1I 74 GCGC G^CGC HincII 136 GTTGAC GT[CT]^[GA]AC HindII 136 GTTGAC GT[CT]^[GA]AC HinfI 733 GATTC G^A[ACGT]TC MseI 314 TTAA T^TAA MslI 690 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 432 ACATGT [GA]CATG^[CT] PinAI 364 ACCGGT A^CCGGT PmaCI 208 CACGTG CAC^GTG PmlI 208 CACGTG CAC^GTG PpuMI 716 GGGACCT [GA]G^G[AT]CC[CT] PshAI 5 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 737 CGGACCG CG^G[AT]CCG SacI 723 GAGCTC GAGCT^C SbfI 225 CCTGCAGG CCTGCA^GG SexAI 246 ACCAGGT A^CC[AT]GGT Sse8387I 225 CCTGCAGG CCTGCA^GG TaqI 569 TCGA T^CGA TfiI 733 GATTC G^A[AT]TC Tru9I 314 TTAA T^TAA Tsp509I 104 AATT ^AATT TthHB8I 569 TCGA T^CGA XcmI 887 CCATCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 432 ACATGT A^C[GA][CT]GT 902 ACACGT AlwI 261 GATCC GGATC(4/5) 867 GATCC AlwNI 169 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 913 CAGCCCCTG AspHI 65 GTGCTC G[AT]GC[AT]^C 723 GAGCTC BanII 350 GGGCTC G[GA]GC[CT]^C 723 GAGCTC BbsI 961 GAAGAC GAAGAC(2/6) 514 GTCTTC BcnI 480 CCGGG CC^[GC]GG 618 CCCGG BpmI 522 CTCCAG CTGGAG(16/14) 162 CTGGAG BpuAI 961 GAAGAC GAAGAC(2/6) 514 GTCTTC BseRI 403 CTCCTC GAGGAG(10/8) 493 CTCCTC BsgI 831 CTGCAC GTGCAG(16/14) 146 GTGCAG BsiHKAI 65 GTGCTC G[AT]GC[AT]^C 723 GAGCTC BspMI 224 ACCTGC ACCTGC(4/8) 510 GCAGGT BsrI 93 ACTGG ACTGG(1/-1) 753 ACTGG BstXI 154 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 669 CCAGCTGGATGG BstYI 260 AGATCC [GA]^GATC[CT] 866 AGATCC EarI 112 CTCTTC CTCTTC(1/4) 840 GAAGAG FauI 686 CCCGC CCCGC(4/6) 24 GCGGG HphI 275 TCACC GGTGA(8/7) 982 TCACC Ksp632I 112 CTCTTC CTCTTC(1/4) 840 GAAGAG MflI 260 AGATCC [GA]^GATC[CT] 866 AGATCC NciI 480 CCGGG CC^[GC]GG 618 CCCGG NlaIII 373 CATG CATG^ 433 CATG PflMI 207 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 912 CCAGCCCCTGG PstI 226 CTGCAG CTGCA^G 508 CTGCAG SfaNI 566 GCATC GCATC(5/9) 689 GCATC StuI 572 AGGCCT AGG^CCT 827 AGGCCT Van91I 207 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 912 CCAGCCCCTGG XhoII 260 AGATCC [GA]^GATC[CT] 866 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AvaII BalI BbvI BfaI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NdeII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I TspRI