Restriction map for
pFN21AE1814
(Circular) (Six-base) MAPSORT of: pFN21AE1814.seq Check: 4719 from: 1 to: 5692
pFN21AE1814 5692 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2018 3502 278
Size: 53 83 186 936 482 1484 2468
Fragments arranged by size:
2468 1484 936 482 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2037 3035 1937
Size: 100 998 4594
Fragments arranged by size:
4594 998 100
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 3755 5139 5407 1632
Size: 833 1290 1384 268 1917
Fragments arranged by size:
1917 1384 1290 833 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2437 2907 4591 5568 1203
Size: 1234 470 1684 977 1327
Fragments arranged by size:
1684 1327 1234 977 470
AgeI A'CCGG_T
Cuts at: 2369 4523 2369
Size: 2154 3538
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2657 2717 1184
Size: 1473 60 4159
Fragments arranged by size:
4159 1473 60
AlwNI CAG_nnn'CTG
Cuts at: 2179 2923 3435 5007 2179
Size: 744 512 1572 2864
Fragments arranged by size:
2864 1572 744 512
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2108 3001 3227 3379 5488 1493
Size: 615 893 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 615 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2609 3017 1870
Size: 73 666 408 4545
Fragments arranged by size:
4545 666 408 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 4468 618
Size: 325 217 627 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2359 2732 3011 1391
Size: 968 373 279 4072
Fragments arranged by size:
4072 968 373 279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2512 2973 928
Size: 971 613 461 3647
Fragments arranged by size:
3647 971 613 461
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 2180 3109 2180
Size: 929 4763
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2512 4352 1239
Size: 949 324 1840 2579
Fragments arranged by size:
2579 1840 949 324
Bpu10I CC'TnA_GC
Cuts at: 2630 2726 2630
Size: 96 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 4355 882
Size: 617 879 1977 2219
Fragments arranged by size:
2219 1977 879 617
BsaAI yAC'GTr
Cuts at: 493 1204 2215 3483 493
Size: 711 1011 1268 2702
Fragments arranged by size:
2702 1268 1011 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3499 3881 4516
Size: 53 83 186 936 482 1484 382 635
Cuts at: 4516 275
Size: 1451
Fragments arranged by size:
1484 1451 936 635 482 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2369 4125 4523 4797 4944 5355 2369
Size: 1756 398 274 147 411 2706
Fragments arranged by size:
2706 1756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 1178
Size: 30 165 30 1311 30 4126
Fragments arranged by size:
4126 1311 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2489 1186
Size: 1213 90 4389
Fragments arranged by size:
4389 1213 90
BseYI C'CCAG_C
Cuts at: 2283 2403 2916 4895 5391 2283
Size: 120 513 1979 496 2584
Fragments arranged by size:
2584 1979 513 496 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2172 2821 1163
Size: 573 436 649 4034
Fragments arranged by size:
4034 649 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 2732 3753 3838 4909 2074
Size: 658 1021 85 1071 2857
Fragments arranged by size:
2857 1071 1021 658 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2359 2732 3011 3753 3838 4909
Size: 399 284 285 373 279 742 85 1071
Cuts at: 4909 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 373 285 284 279
85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2506 3047 844
Size: 1394 268 541 3489
Fragments arranged by size:
3489 1394 541 268
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 4187 4361 65
Size: 1183 1179 1760 174 1396
Fragments arranged by size:
1760 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2369 4336 4523 1700
Size: 110 559 1967 187 2869
Fragments arranged by size:
2869 1967 559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2166 2681 2166
Size: 515 5177
BstYI r'GATC_y
Cuts at: 1972 2265 2871 3022 3389 3774 3791 5243 5329
Size: 293 606 151 367 385 17 1452 86
Cuts at: 5329 5341 5687 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 606 385 367 346 293 151
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 3208 4002 4022 2292
Size: 916 794 20 3962
Fragments arranged by size:
3962 916 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2209 2466
Size: 54 1425 50 275 18 219 160 257
Cuts at: 2466 2862 2925 3139 4028 5657 8
Size: 396 63 214 889 1629 43
Fragments arranged by size:
1629 1425 889 396 275 257 219 214
160 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 2840 3622 1353
Size: 770 717 782 3423
Fragments arranged by size:
3423 782 770 717
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2864 4279 4653 4799 387
Size: 1064 138 1275 1415 374 146 1280
Fragments arranged by size:
1415 1280 1275 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2730 2730
Size: 5692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2465 2512 3755 4352 5139 5407
Size: 393 556 277 47 1243 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 1243 787 597 556 393 277 268
47
EcoNI CCTnn'n_nnAGG
Cuts at: 2051 2051
Size: 5692
EcoO109I rG'GnC_Cy
Cuts at: 2722 2722
Size: 5692
EcoRI G'AATT_C
Cuts at: 2108 3001 3379 2108
Size: 893 378 4421
Fragments arranged by size:
4421 893 378
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2082 4839 1678
Size: 404 2757 2531
Fragments arranged by size:
2757 2531 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2714 2746 4333 4365 4407
Size: 32 135 32 1142 32 1587 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 1142 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 3036 3282 669
Size: 988 281 205 893 246 3079
Fragments arranged by size:
3079 988 893 281 246 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 4806 4990 1305
Size: 832 2669 184 2007
Fragments arranged by size:
2669 2007 832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 2468 2864 10
Size: 54 1425 343 219 417 396 2838
Fragments arranged by size:
2838 1425 417 396 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 3650 4009 4168 518
Size: 648 1533 951 359 159 2042
Fragments arranged by size:
2042 1533 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2166 2287 2512 2677 2860 3108 3785 4933
Size: 560 121 225 165 183 248 677 1148
Cuts at: 4933 5178 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 560 248 245 225 183
165 121
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2441 3050 4595 1132
Size: 97 1212 609 1545 2229
Fragments arranged by size:
2229 1545 1212 609 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 2437 4591 2437
Size: 2154 3538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2218 2923 1108
Size: 1110 705 3877
Fragments arranged by size:
3877 1110 705
PfoI T'CCnGG_A
Cuts at: 2622 2622
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PmlI CAC'GTG
Cuts at: 2215 2215
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2722 2722
Size: 5692
PshAI GACnn'nnGTC
Cuts at: 1613 2014 1613
Size: 401 5291
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2235 2517 3044 830
Size: 1405 282 527 3478
Fragments arranged by size:
3478 1405 527 282
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2166 2287 2512 2677 2860 2166
Size: 121 225 165 183 4998
Fragments arranged by size:
4998 225 183 165 121
RsrII CG'GwC_CG
Cuts at: 2743 2743
Size: 5692
SacI G_AGCT'C
Cuts at: 2732 2732
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 2235 3044 2235
Size: 809 4883
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SexAI A'CCwGG_T
Cuts at: 2251 2251
Size: 5692
SfcI C'TryA_G
Cuts at: 826 1045 2231 2503 2513 3040 4175 4856 5047
Size: 219 1186 272 10 527 1135 681 191
Cuts at: 5047 826
Size: 1471
Fragments arranged by size:
1471 1186 1135 681 527 272 219 191
10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 2834 1517
Size: 1062 255 4375
Fragments arranged by size:
4375 1062 255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2122 2200 2298 2865 3131
Size: 552 939 48 70 78 98 567 266
Cuts at: 3131 513
Size: 3074
Fragments arranged by size:
3074 939 567 552 266 98 78 70
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 2812 3719 3736 3889 4074 4519
Size: 1255 717 515 907 17 153 185 445
Cuts at: 4519 325
Size: 1498
Fragments arranged by size:
1498 1255 907 717 515 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 2787 3938
Size: 274 80 33 51 1443 804 6 1151
Cuts at: 3938 5650 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1151 804 274 138 80 51
33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2899 2899
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2016 3500 276
Size: 53 83 186 936 482 1484 2468
Fragments arranged by size:
2468 1484 936 482 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI SacII SanDI SapI SfiI
SfoI SrfI SwaI