(Linear) (Six-base) MAPSORT of: KIEE1814.seq Check: 4509 from: 1 to: 987 KIEE1814.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AatII G_ACGT'C Cuts at: 0 13 987 Size: 13 974 AccI GT'mk_AC Cuts at: 0 32 987 Size: 32 955 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 987 Size: 460 527 AflIII A'CryG_T Cuts at: 0 432 902 987 Size: 432 470 85 Fragments arranged by size: 470 432 85 AgeI A'CCGG_T Cuts at: 0 364 987 Size: 364 623 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 652 712 987 Size: 652 60 275 Fragments arranged by size: 652 275 60 AlwNI CAG_nnn'CTG Cuts at: 0 174 918 987 Size: 174 744 69 Fragments arranged by size: 744 174 69 ApoI r'AATT_y Cuts at: 0 103 987 Size: 103 884 AvaI C'yCGr_G Cuts at: 0 604 987 Size: 604 383 BanII G_rGCy'C Cuts at: 0 354 727 987 Size: 354 373 260 Fragments arranged by size: 373 354 260 BbsI GAAGACnn'nnnn_ Cuts at: 0 507 968 987 Size: 507 461 19 Fragments arranged by size: 507 461 19 BlpI GC'TnA_GC Cuts at: 0 175 987 Size: 175 812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 786 987 Size: 786 201 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 507 987 Size: 183 324 480 Fragments arranged by size: 480 324 183 Bpu10I CC'TnA_GC Cuts at: 0 625 721 987 Size: 625 96 266 Fragments arranged by size: 625 266 96 BsaI GGTCTCn'nnnn_ Cuts at: 0 373 987 Size: 373 614 BsaAI yAC'GTr Cuts at: 0 210 987 Size: 210 777 BsaHI Gr'CG_yC Cuts at: 0 10 987 Size: 10 977 BsaWI w'CCGG_w Cuts at: 0 364 987 Size: 364 623 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 709 739 987 Size: 709 30 248 Fragments arranged by size: 709 248 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 394 484 987 Size: 394 90 503 Fragments arranged by size: 503 394 90 BseYI C'CCAG_C Cuts at: 0 278 398 911 987 Size: 278 120 513 76 Fragments arranged by size: 513 278 120 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 167 816 987 Size: 167 649 171 Fragments arranged by size: 649 171 167 BsiHKAI G_wGCw'C Cuts at: 0 69 727 987 Size: 69 658 260 Fragments arranged by size: 658 260 69 Bsp1286I G_dGCh'C Cuts at: 0 69 354 727 987 Size: 69 285 373 260 Fragments arranged by size: 373 285 260 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 233 501 987 Size: 233 268 486 Fragments arranged by size: 486 268 233 BsrDI GCAATG_nn' Cuts at: 0 422 987 Size: 422 565 BsrFI r'CCGG_y Cuts at: 0 364 987 Size: 364 623 BstXI CCAn_nnnn'nTGG Cuts at: 0 161 676 987 Size: 161 515 311 Fragments arranged by size: 515 311 161 BstYI r'GATC_y Cuts at: 0 260 866 987 Size: 260 606 121 Fragments arranged by size: 606 260 121 BtsI GCAGTG_nn' Cuts at: 0 287 987 Size: 287 700 DraIII CAC_nnn'GTG Cuts at: 0 213 987 Size: 213 774 EaeI y'GGCC_r Cuts at: 0 44 204 461 857 920 987 Size: 44 160 257 396 63 67 Fragments arranged by size: 396 257 160 67 63 44 EarI CTCTTCn'nnn_ Cuts at: 0 118 835 987 Size: 118 717 152 Fragments arranged by size: 717 152 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 859 987 Size: 859 128 EcoICRI GAG'CTC Cuts at: 0 725 987 Size: 725 262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 460 507 987 Size: 183 277 47 480 Fragments arranged by size: 480 277 183 47 EcoNI CCTnn'n_nnAGG Cuts at: 0 46 987 Size: 46 941 EcoO109I rG'GnC_Cy Cuts at: 0 717 987 Size: 717 270 EcoRI G'AATT_C Cuts at: 0 103 987 Size: 103 884 HaeII r_GCGC'y Cuts at: 0 77 987 Size: 77 910 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 709 741 987 Size: 709 32 246 Fragments arranged by size: 709 246 32 HincII GTy'rAC Cuts at: 0 138 987 Size: 138 849 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 132 987 Size: 132 855 MscI TGG'CCA Cuts at: 0 46 463 859 987 Size: 46 417 396 128 Fragments arranged by size: 417 396 128 46 MslI CAynn'nnrTG Cuts at: 0 694 987 Size: 694 293 MspA1I CmG'CkG Cuts at: 0 161 282 507 672 855 987 Size: 161 121 225 165 183 132 Fragments arranged by size: 225 183 165 161 132 121 NspI r_CATG'y Cuts at: 0 436 987 Size: 436 551 PciI A'CATG_T Cuts at: 0 432 987 Size: 432 555 PflMI CCAn_nnn'nTGG Cuts at: 0 213 918 987 Size: 213 705 69 Fragments arranged by size: 705 213 69 PfoI T'CCnGG_A Cuts at: 0 617 987 Size: 617 370 PmlI CAC'GTG Cuts at: 0 210 987 Size: 210 777 PpuMI rG'GwC_Cy Cuts at: 0 717 987 Size: 717 270 PshAI GACnn'nnGTC Cuts at: 0 9 987 Size: 9 978 PstI C_TGCA'G Cuts at: 0 230 512 987 Size: 230 282 475 Fragments arranged by size: 475 282 230 PvuII CAG'CTG Cuts at: 0 161 282 507 672 855 987 Size: 161 121 225 165 183 132 Fragments arranged by size: 225 183 165 161 132 121 RsrII CG'GwC_CG Cuts at: 0 738 987 Size: 738 249 SacI G_AGCT'C Cuts at: 0 727 987 Size: 727 260 SbfI CC_TGCA'GG Cuts at: 0 230 987 Size: 230 757 SexAI A'CCwGG_T Cuts at: 0 246 987 Size: 246 741 SfcI C'TryA_G Cuts at: 0 226 498 508 987 Size: 226 272 10 479 Fragments arranged by size: 479 272 226 10 StuI AGG'CCT Cuts at: 0 574 829 987 Size: 574 255 158 Fragments arranged by size: 574 255 158 StyI C'CwwG_G Cuts at: 0 47 117 195 293 860 987 Size: 47 70 78 98 567 127 Fragments arranged by size: 567 127 98 78 70 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 292 807 987 Size: 292 515 180 Fragments arranged by size: 515 292 180 TatI w'GTAC_w Cuts at: 0 776 782 987 Size: 776 6 205 Fragments arranged by size: 776 205 6 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 894 987 Size: 894 93 ZraI GAC'GTC Cuts at: 0 11 987 Size: 11 976 Enzymes that do cut: AatII AccI AcuI AflIII AgeI AlfI AlwNI ApoI AvaI BanII BbsI BlpI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII MmeI MscI MslI MspA1I NspI PciI PflMI PfoI PmlI PpuMI PshAI PstI PvuII RsrII SacI SbfI SexAI SfcI StuI StyI TaqII TatI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI