Restriction map for
pFN21AE5265
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrGI
BsrI BssHII BssKI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoT14I EcoT22I FauI FseI HaeII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvaI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 469 GAATTC [GA]^AATT[CT]
AcyI 391 GACGCC G[GA]^CG[CT]C
ApoI 469 GAATTC [GA]^AATT[CT]
AspI 405 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 497 TGCGCA TGC^GCA
BanI 210 GGTGCC G^G[CT][GA]CC
BclI 503 TGATCA T^GATCA
BpmI 323 CTGGAG CTGGAG(16/14)
BsaAI 551 TACGTA [CT]AC^GT[GA]
BsaHI 391 GACGCC G[GA]^CG[CT]C
BsaJI 161 CCTCGG C^C[ACGT][ACGT]GG
BsgI 310 CTGCAC GTGCAG(16/14)
BsiWI 301 CGTACG C^GTACG
BsmAI 239 GTCTC GTCTC(1/5)
BsmBI 238 CGTCTC CGTCTC(1/5)
BsmI 194 GCATTC GAATGC(1/-1)
BspDI 283 ATCGAT AT^CGAT
BspHI 362 TCATGA T^CATGA
BsrFI 513 GCCGGT [GA]^CCGG[CT]
BssSI 227 CTCGTG CACGAG(-5/-1)
Cfr10I 513 GCCGGT [GA]^CCGG[CT]
ClaI 283 ATCGAT AT^CGAT
DraII 545 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 104 TGGCCG [CT]^GGCC[GA]
EcoO109I 545 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 469 GAATTC G^AATTC
EcoRV 561 GATATC GAT^ATC
FbaI 503 TGATCA T^GATCA
FspI 497 TGCGCA TGC^GCA
Hin1I 391 GACGCC G[GA]^CG[CT]C
HincII 175 GTCAAC GT[CT]^[GA]AC
HindII 175 GTCAAC GT[CT]^[GA]AC
HindIII 81 AAGCTT A^AGCTT
HinfI 232 GACTC G^A[ACGT]TC
HphI 143 TCACC GGTGA(8/7)
MboII 185 TCTTC GAAGA(8/7)
MseI 482 TTAA T^TAA
NlaIV 210 GGTGCC GG[ACGT]^[ACGT]CC
PflFI 405 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 232 GACTC GAGTC(4/5)
PpuMI 545 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 232 GACTCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 362 TCATGA T^CATGA
SfcI 57 CTACAG C^T[GA][CT]AG
SnaBI 551 TACGTA TAC^GTA
Tru9I 482 TTAA T^TAA
Tsp45I 230 GTGAC ^GT[GC]AC
Tth111I 405 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 377 CCATGTCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 404 GGACC G^G[AT]CC
546 GGACC
BsrDI 261 CATTGC GCAATG(2/0)
95 GCAATG
CfoI 414 GCGC GCG^C
498 GCGC
DdeI 32 CTCAG C^T[ACGT]AG
91 CTCAG
DpnII 268 GATC ^GATC
504 GATC
HapII 429 CCGG C^CGG
514 CCGG
HhaI 414 GCGC GCG^C
498 GCGC
HinP1I 414 GCGC G^CGC
498 GCGC
HpaII 429 CCGG C^CGG
514 CCGG
MaeII 179 ACGT A^CGT
552 ACGT
MboI 268 GATC ^GATC
504 GATC
MslI 204 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
282 CATCGATATG
MspI 429 CCGG C^CGG
514 CCGG
NdeII 268 GATC ^GATC
504 GATC
Sau3AI 268 GATC ^GATC
504 GATC
TaqI 188 TCGA T^CGA
284 TCGA
Tsp509I 463 AATT ^AATT
470 AATT
TspRI 313 CACTG CA[GC]TG[ACGT][ACGT]^
344 CACTG
TthHB8I 188 TCGA T^CGA
284 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BsiYI BslI BstF5I BstUI Cfr13I
Fnu4HI FokI HaeIII HgaI ItaI
MaeIII MnlI MvnI NlaIII RsaI
Sau96I SfaNI TseI