(Circular) (Six-base) MAPSORT of: pFN21AE5265.seq Check: 3706 from: 1 to: 5275 pFN21AE5265 5275 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3085 278 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2618 1937 Size: 681 4594 Acc65I G'GTAC_C Cuts at: 2596 2596 Size: 5275 AclI AA'CG_TT Cuts at: 1563 3402 3775 1563 Size: 1839 373 3063 Fragments arranged by size: 3063 1839 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3338 4722 4990 1632 Size: 1706 1384 268 1917 Fragments arranged by size: 1917 1706 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5078 AflIII A'CryG_T Cuts at: 1203 4174 5151 1203 Size: 2971 977 1327 Fragments arranged by size: 2971 1327 977 AgeI A'CCGG_T Cuts at: 4106 4106 Size: 5275 AhdI GACnn_n'nnGTC Cuts at: 4004 4004 Size: 5275 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5275 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5275 AlwNI CAG_nnn'CTG Cuts at: 3018 4590 3018 Size: 1572 3703 ApaLI G'TGCA_C Cuts at: 3332 4488 3332 Size: 1156 4119 ApoI r'AATT_y Cuts at: 1493 2474 2584 2810 2962 5071 1493 Size: 981 110 226 152 2109 1697 Fragments arranged by size: 2109 1697 981 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 3829 160 Size: 872 2797 1606 Fragments arranged by size: 2797 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 AvaI C'yCGr_G Cuts at: 1870 1943 2600 1870 Size: 73 657 4545 Fragments arranged by size: 4545 657 73 BamHI G'GATC_C Cuts at: 2605 2972 2605 Size: 367 4908 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2215 2596 4051 618 Size: 325 217 627 428 381 1455 1842 Fragments arranged by size: 1842 1455 627 428 381 325 217 BanII G_rGCy'C Cuts at: 1391 2594 1391 Size: 1203 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3466 3500 1903 Size: 34 1529 34 3678 Fragments arranged by size: 3678 1529 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3167 4377 3167 Size: 1210 4065 BclI T'GATC_A Cuts at: 1548 2508 1548 Size: 960 4315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3886 136 Size: 107 122 71 631 605 2214 1525 Fragments arranged by size: 2214 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5270 5270 Size: 5275 BlpI GC'TnA_GC Cuts at: 2692 2692 Size: 5275 Bme1580I G_kGCm'C Cuts at: 1790 3336 4492 1790 Size: 1546 1156 2573 Fragments arranged by size: 2573 1546 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5275 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3006 3964 442 Size: 416 53 83 399 24 1589 958 1753 Fragments arranged by size: 1753 1589 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5275 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2349 3935 1239 Size: 1110 1586 2579 Fragments arranged by size: 2579 1586 1110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3400 4265 4563 3400 Size: 865 298 4112 Fragments arranged by size: 4112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3938 882 Size: 617 2439 2219 Fragments arranged by size: 2439 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2558 3066 493 Size: 711 1354 508 2702 Fragments arranged by size: 2702 1354 711 508 BsaBI GATnn'nnATC Cuts at: 5269 5269 Size: 5275 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2397 3082 3464 4099 Size: 53 83 186 936 864 685 382 635 Cuts at: 4099 275 Size: 1451 Fragments arranged by size: 1451 936 864 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3708 4106 4380 4527 4938 3708 Size: 398 274 147 411 4045 Fragments arranged by size: 4045 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 1178 Size: 30 165 30 651 30 4369 Fragments arranged by size: 4369 651 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5275 BseYI C'CCAG_C Cuts at: 4478 4974 4478 Size: 496 4779 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 4138 Fragments arranged by size: 4138 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 3486 4514 1540 Size: 275 186 724 761 1028 2301 Fragments arranged by size: 2301 1028 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 3336 3421 4492 3336 Size: 85 1071 4119 Fragments arranged by size: 4119 1071 85 BsiWI C'GTAC_G Cuts at: 2306 2306 Size: 5275 BsmI GAATG_Cn' Cuts at: 2199 2786 2879 2199 Size: 587 93 4595 Fragments arranged by size: 4595 587 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2249 2249 Size: 5275 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2594 3336 3421 4492 1391 Size: 399 804 742 85 1071 2174 Fragments arranged by size: 2174 1071 804 742 399 85 BspEI T'CCGG_A Cuts at: 4938 4938 Size: 5275 BspHI T'CATG_A Cuts at: 2367 3164 4894 2367 Size: 797 1730 2748 Fragments arranged by size: 2748 1730 797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2630 844 Size: 1786 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3162 1394 Size: 429 1339 3507 Fragments arranged by size: 3507 1339 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2107 2265 3770 3944 65 Size: 1183 859 158 1505 174 1396 Fragments arranged by size: 1505 1396 1183 859 174 158 BsrFI r'CCGG_y Cuts at: 1700 1810 2518 3919 4106 1700 Size: 110 708 1401 187 2869 Fragments arranged by size: 2869 1401 708 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5275 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5275 BssSI C'ACGA_G Cuts at: 2232 3335 4347 2232 Size: 1103 1012 3160 Fragments arranged by size: 3160 1103 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5275 BstBI TT'CG_AA Cuts at: 4079 4079 Size: 5275 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5275 BstYI r'GATC_y Cuts at: 1972 2605 2972 3357 3374 4826 4912 4924 5270 Size: 633 367 385 17 1452 86 12 346 Cuts at: 5270 1972 Size: 1977 Fragments arranged by size: 1977 1452 633 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4723 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4536 Fragments arranged by size: 4536 683 56 BtsI GCAGTG_nn' Cuts at: 2317 2791 3585 3605 2317 Size: 474 794 20 3987 Fragments arranged by size: 3987 794 474 20 ClaI AT'CG_AT Cuts at: 1554 2289 1554 Size: 735 4540 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5240 DraI TTT'AAA Cuts at: 2579 2579 Size: 5275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4282 809 Size: 507 2966 1802 Fragments arranged by size: 2966 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2722 3611 Size: 54 1425 50 275 18 279 613 889 Cuts at: 3611 5240 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 613 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2722 1537 Size: 275 910 4090 Fragments arranged by size: 4090 910 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3205 1353 Size: 1852 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2332 3862 4236 4382 387 Size: 1064 138 743 1530 374 146 1280 Fragments arranged by size: 1530 1280 1064 743 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 3338 3935 4722 4990 1239 Size: 393 717 989 597 787 268 1524 Fragments arranged by size: 1524 989 787 717 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2551 2551 Size: 5275 EcoRI G'AATT_C Cuts at: 2474 2584 2962 2474 Size: 110 378 4787 Fragments arranged by size: 4787 378 110 EcoRV GAT'ATC Cuts at: 2568 2568 Size: 5275 FspI TGC'GCA Cuts at: 2504 3781 2504 Size: 1277 3998 FspAI rTGC'GCAy Cuts at: 2504 2504 Size: 5275 HaeII r_GCGC'y Cuts at: 1678 4422 1678 Size: 2744 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3916 3948 3990 4022 1373 Size: 32 135 32 2344 32 42 32 2626 Fragments arranged by size: 2626 2344 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 2619 2865 669 Size: 988 281 244 437 246 3079 Fragments arranged by size: 3079 988 437 281 246 244 HindIII A'AGCT_T Cuts at: 748 2086 748 Size: 1338 3937 HpaI GTT'AAC Cuts at: 2865 2865 Size: 5275 KpnI G_GTAC'C Cuts at: 2600 2600 Size: 5275 MfeI C'AATT_G Cuts at: 2874 2874 Size: 5275 MluI A'CGCG_T Cuts at: 5151 5151 Size: 5275 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4389 4573 1305 Size: 3084 184 2007 Fragments arranged by size: 3084 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3453 Fragments arranged by size: 3453 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2291 3233 3592 3751 518 Size: 648 1047 78 942 359 159 2042 Fragments arranged by size: 2042 1047 942 648 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2691 3368 4516 4761 1606 Size: 1085 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4723 NdeI CA'TA_TG Cuts at: 387 387 Size: 5275 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5275 NotI GC'GGCC_GC Cuts at: 2722 2722 Size: 5275 NspI r_CATG'y Cuts at: 1132 1229 2633 4178 1132 Size: 97 1404 1545 2229 Fragments arranged by size: 2229 1545 1404 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5275 PciI A'CATG_T Cuts at: 4174 4174 Size: 5275 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5275 PmeI GTTT'AAAC Cuts at: 2579 2579 Size: 5275 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3344 3376 4857 4889 3344 Size: 32 1481 32 3730 Fragments arranged by size: 3730 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2551 2551 Size: 5275 PshAI GACnn'nnGTC Cuts at: 1613 2241 1613 Size: 628 4647 PsiI TTA'TAA Cuts at: 2845 2845 Size: 5275 PstI C_TGCA'G Cuts at: 830 2627 830 Size: 1797 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5275 SalI G'TCGA_C Cuts at: 1936 2617 1936 Size: 681 4594 SbfI CC_TGCA'GG Cuts at: 2627 2627 Size: 5275 ScaI AGT'ACT Cuts at: 3523 5235 3523 Size: 1712 3563 SfcI C'TryA_G Cuts at: 826 1045 2062 2623 3758 4439 4630 826 Size: 219 1017 561 1135 681 191 1471 Fragments arranged by size: 1471 1135 1017 681 561 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5275 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5275 SmaI CCC'GGG Cuts at: 1872 2602 1872 Size: 730 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3379 4280 4542 820 Size: 197 926 1436 901 262 1553 Fragments arranged by size: 1553 1436 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2558 3066 493 Size: 2065 508 2702 Fragments arranged by size: 2702 2065 508 SpeI A'CTAG_T Cuts at: 152 152 Size: 5275 SphI G_CATG'C Cuts at: 1132 2633 1132 Size: 1501 3774 SspI AAT'ATT Cuts at: 5 52 3199 5 Size: 47 3147 2081 Fragments arranged by size: 3147 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5275 StyI C'CwwG_G Cuts at: 513 1065 2714 513 Size: 552 1649 3074 Fragments arranged by size: 3074 1649 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3302 3319 3472 3657 4102 325 Size: 1255 1722 17 153 185 445 1498 Fragments arranged by size: 1722 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2205 3521 5233 Size: 274 80 33 51 1443 228 1316 1712 Cuts at: 5233 96 Size: 138 Fragments arranged by size: 1712 1443 1316 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5235 5267 5235 Size: 32 5243 Tth111I GACn'n_nGTC Cuts at: 1316 2413 1316 Size: 1097 4178 XbaI T'CTAG_A Cuts at: 2611 2611 Size: 5275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 5275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5275 XmaI C'CCGG_G Cuts at: 1870 2600 1870 Size: 730 4545 XmnI GAAnn'nnTTC Cuts at: 3404 3404 Size: 5275 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3083 276 Size: 53 83 186 936 1549 2468 Fragments arranged by size: 2468 1549 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI