################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BlnI BsaAI BsaBI BseAI BsiWI BspDI BspEI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraII DrdI Eam1105I EcoO109I EcoO65I EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 4035 CGCG CG^CG AflII 1486 CTTAAG C^TTAAG AflIII 2759 ACATGT A^C[GA][CT]GT Alw44I 3949 GTGCAC G^TGCAC AocI 3936 CCTCAGG CC^T[ACGT]AGG Aor51HI 3497 AGCGCT AGC^GCT ApaLI 3949 GTGCAC G^TGCAC AspI 1207 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3325 TGGCCA TGG^CCA BanI 2815 GGTGCC G^G[CT][GA]CC BbvCI 3493 CCTCAGC CCTCAGC(-5/-2) BciVI 2462 GTATCC GTATCC(6/5) BclI 3716 TGATCA T^GATCA BfrI 1486 CTTAAG C^TTAAG BglII 618 AGATCT A^GATCT BlpI 150 GCTGAGC GC^T[ACGT]AGC Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC BsaI 962 GAGACC GGTCTC(1/5) BsaWI 3408 TCCGGT [AT]^CCGG[AT] BsiEI 116 CGGCCG CG[GA][CT]^CG BsmBI 3648 GAGACG CGTCTC(1/5) Bsp1407I 875 TGTACA T^GTACA BspHI 3644 TCATGA T^CATGA BspLU11I 2759 ACATGT A^CATGT BsrBI 2106 CCGCTC CCGCTC(-3/-3) BsrFI 146 ACCGGC [GA]^CCGG[CT] BsrGI 875 TGTACA T^GTACA BssHII 4034 GCGCGC G^CGCGC BstUI 4035 CGCG CG^CG Bsu36I 3936 CCTCAGG CC^T[ACGT]AGG CelII 150 GCTGAGC GC^T[ACGT]AGC Cfr10I 146 ACCGGC [GA]^CCGG[CT] DraI 3880 TTTAAA TTT^AAA DraIII 3302 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 116 CGGCCG C^GGCCG EclXI 116 CGGCCG C^GGCCG Eco47III 3497 AGCGCT AGC^GCT Eco52I 116 CGGCCG C^GGCCG Eco81I 3936 CCTCAGG CC^T[ACGT]AGG EcoRI 2472 GAATTC G^AATTC FbaI 3716 TGATCA T^GATCA HaeII 3497 AGCGCT [GA]GCGC^[CT] HindIII 357 AAGCTT A^AGCTT MfeI 1390 CAATTG C^AATTG MluNI 3325 TGGCCA TGG^CCA MscI 3325 TGGCCA TGG^CCA MunI 1390 CAATTG C^AATTG MvnI 4035 CGCG CG^CG NcoI 730 CCATGG C^CATGG NotI 115 GCGGCCGC GC^GGCCGC PaeR7I 3434 CTCGAG C^TCGAG PflFI 1207 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2787 GAGTC GAGTC(4/5) PshAI 68 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3644 TCATGA T^CATGA ScaI 1700 AGTACT AGT^ACT SpeI 2844 ACTAGT A^CTAGT SspBI 875 TGTACA T^GTACA StuI 3576 AGGCCT AGG^CCT Tth111I 1207 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 377 CCATCCTGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3434 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BbsI 1735 GAAGAC GAAGAC(2/6) 737 GTCTTC BglI 103 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 106 GCCGTGGCGGC BpuAI 1735 GAAGAC GAAGAC(2/6) 737 GTCTTC BsmI 1719 GAATGC GAATGC(1/-1) 4294 GAATGC HincII 204 GTTGAC GT[CT]^[GA]AC 2899 GTTGAC HindII 204 GTTGAC GT[CT]^[GA]AC 2899 GTTGAC NdeI 464 CATATG CA^TATG 2243 CATATG NheI 133 GCTAGC G^CTAGC 265 GCTAGC SacI 1223 GAGCTC GAGCT^C 3920 GAGCTC SmaI 96 CCCGGG CCC^GGG 138 CCCGGG SspI 2383 AATATT AAT^ATT 4302 AATATT XmaCI 96 CCCGGG C^CCGGG 138 CCCGGG XmaI 96 CCCGGG C^CCGGG 138 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI