Restriction map for
pFN21AB9455
(Circular) (Six-base) MAPSORT of: pFN21AB9455.seq Check: 830 from: 1 to: 9067
pFN21AB9455 9067 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2968 2968
Size: 9067
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6877 278
Size: 53 83 186 936 5341 2468
Fragments arranged by size:
5341 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6410 1937
Size: 4473 4594
Acc65I G'GTAC_C
Cuts at: 6388 6388
Size: 9067
AclI AA'CG_TT
Cuts at: 1563 7194 7567 1563
Size: 5631 373 3063
Fragments arranged by size:
5631 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2855 2897 3272 4021 4741 5771 6165 6281
Size: 1223 42 375 749 720 1030 394 116
Cuts at: 6281 7130 8514 8782 1632
Size: 849 1384 268 1917
Fragments arranged by size:
1917 1384 1223 1030 849 749 720 394
375 268 116 42
AfeI AGC'GCT
Cuts at: 5504 5504
Size: 9067
AflII C'TTAA_G
Cuts at: 820 1017 3491 820
Size: 197 2474 6396
Fragments arranged by size:
6396 2474 197
AflIII A'CryG_T
Cuts at: 1203 4764 7966 8943 1203
Size: 3561 3202 977 1327
Fragments arranged by size:
3561 3202 1327 977
AgeI A'CCGG_T
Cuts at: 7898 7898
Size: 9067
AhdI GACnn_n'nnGTC
Cuts at: 7796 7796
Size: 9067
AleI CACnn'nnGTG
Cuts at: 1166 5300 1166
Size: 4134 4933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5764 1184
Size: 4580 4487
AlwNI CAG_nnn'CTG
Cuts at: 3062 3146 4542 4618 6810 8382 3062
Size: 84 1396 76 2192 1572 3747
Fragments arranged by size:
3747 2192 1572 1396 84 76
ApaLI G'TGCA_C
Cuts at: 5954 7124 8280 5954
Size: 1170 1156 6741
Fragments arranged by size:
6741 1170 1156
ApoI r'AATT_y
Cuts at: 1493 2171 2690 2757 4062 4349 4477 4493 5701
Size: 678 519 67 1305 287 128 16 1208
Cuts at: 5701 5852 6376 6602 6754 8863 1493
Size: 151 524 226 152 2109 1697
Fragments arranged by size:
2109 1697 1305 1208 678 524 519 287
226 152 151 128 67 16
AseI AT'TA_AT
Cuts at: 160 1032 7621 160
Size: 872 6589 1606
Fragments arranged by size:
6589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9067
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 2143 5439 6392 1870
Size: 73 158 42 3296 953 4545
Fragments arranged by size:
4545 3296 953 158 73 42
BamHI G'GATC_C
Cuts at: 6397 6764 6397
Size: 367 8700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4820 6388 7843 618
Size: 325 217 627 3033 1568 1455 1842
Fragments arranged by size:
3033 1842 1568 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3232 3339 5182 5929 6386 1391
Size: 1841 107 1843 747 457 4072
Fragments arranged by size:
4072 1843 1841 747 457 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2735 3747 928
Size: 971 836 1012 6248
Fragments arranged by size:
6248 1012 971 836
BbvCI CC'TCA_GC
Cuts at: 5499 5499
Size: 9067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7258 7292 1903
Size: 34 5321 34 3678
Fragments arranged by size:
5321 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4478 6959 8169 4478
Size: 2481 1210 5376
Fragments arranged by size:
5376 2481 1210
BclI T'GATC_A
Cuts at: 1548 5721 1548
Size: 4173 4894
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 2117 7678
Size: 107 122 71 631 605 442 3 5561
Cuts at: 7678 136
Size: 1525
Fragments arranged by size:
5561 1525 631 605 442 122 107 71
3
BglII A'GATC_T
Cuts at: 2623 9062 2623
Size: 6439 2628
BlpI GC'TnA_GC
Cuts at: 2156 6484 2156
Size: 4328 4739
Bme1580I G_kGCm'C
Cuts at: 1790 5592 5958 7128 8284 1790
Size: 3802 366 1170 1156 2573
Fragments arranged by size:
3802 2573 1170 1156 366
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2006 5157 6798
Size: 416 53 83 399 24 589 3151 1641
Cuts at: 6798 7756 442
Size: 958 1753
Fragments arranged by size:
3151 1753 1641 958 589 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2142 2274 1056
Size: 1086 132 7849
Fragments arranged by size:
7849 1086 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2526 4016 5118 5562 5990 7727 1239
Size: 803 484 1490 1102 444 428 1737 2579
Fragments arranged by size:
2579 1737 1490 1102 803 484 444 428
Bpu10I CC'TnA_GC
Cuts at: 5499 5499
Size: 9067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2732 5328 5645 7192 8057 8355 2732
Size: 2596 317 1547 865 298 3444
Fragments arranged by size:
3444 2596 1547 865 317 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2961 7730 882
Size: 617 1462 4769 2219
Fragments arranged by size:
4769 2219 1462 617
BsaAI yAC'GTr
Cuts at: 493 1204 6858 493
Size: 711 5654 2702
Fragments arranged by size:
5654 2702 711
BsaBI GATnn'nnATC
Cuts at: 9061 9061
Size: 9067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2740 6065 6361 6874
Size: 53 83 186 936 1207 3325 296 513
Cuts at: 6874 7256 7891 275
Size: 382 635 1451
Fragments arranged by size:
3325 1451 1207 936 635 513 382 296
186 83 53
BsaWI w'CCGG_w
Cuts at: 5413 7500 7898 8172 8319 8730 5413
Size: 2087 398 274 147 411 5750
Fragments arranged by size:
5750 2087 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2097 2127 2958 2988 4005
Size: 30 165 30 694 30 831 30 1017
Cuts at: 4005 4035 4618 4648 4783 4813 5116 5146 6016
Size: 30 583 30 135 30 303 30 870
Cuts at: 6016 6046 1178
Size: 30 4199
Fragments arranged by size:
4199 1017 870 831 694 583 303 165
135 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2622 4986 5199 5331 1186
Size: 899 537 2364 213 132 4922
Fragments arranged by size:
4922 2364 899 537 213 132
BseYI C'CCAG_C
Cuts at: 2086 3310 5016 6035 6192 8270 8766 2086
Size: 1224 1706 1019 157 2078 496 2387
Fragments arranged by size:
2387 2078 1706 1224 1019 496 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2165 2282 2858 2996 3288 3344
Size: 573 300 129 117 576 138 292 56
Cuts at: 3344 4854 1163
Size: 1510 5376
Fragments arranged by size:
5376 1510 576 573 300 292 138 129
117 56
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2124 6517 7278 8306 1540
Size: 275 186 123 4393 761 1028 2301
Fragments arranged by size:
4393 2301 1028 761 275 186 123
BsiHKAI G_wGCw'C
Cuts at: 2162 3232 5929 5958 7128 7213 8284 2162
Size: 1070 2697 29 1170 85 1071 2945
Fragments arranged by size:
2945 2697 1170 1071 1070 85 29
BsmI GAATG_Cn'
Cuts at: 3730 6305 6578 6671 3730
Size: 2575 273 93 6126
Fragments arranged by size:
6126 2575 273 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5647 5647
Size: 9067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 3232 3339 5182 5592 5929 5958
Size: 399 372 1070 107 1843 410 337 29
Cuts at: 5958 6386 7128 7213 8284 1391
Size: 428 742 85 1071 2174
Fragments arranged by size:
2174 1843 1071 1070 742 428 410 399
372 337 107 85 29
BspEI T'CCGG_A
Cuts at: 8730 8730
Size: 9067
BspHI T'CATG_A
Cuts at: 5649 6956 8686 5649
Size: 1307 1730 6030
Fragments arranged by size:
6030 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2525 2968 4384 5591 6422 844
Size: 1681 443 1416 1207 831 3489
Fragments arranged by size:
3489 1681 1416 1207 831 443
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 6954 1394
Size: 429 2290 2841 3507
Fragments arranged by size:
3507 2841 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3560 3758 3759 4320 7562 7736 65
Size: 1183 2312 198 1 561 3242 174 1396
Fragments arranged by size:
3242 2312 1396 1183 561 198 174 1
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 7711 7898 1700
Size: 110 341 5560 187 2869
Fragments arranged by size:
5560 2869 341 187 110
BsrGI T'GTAC_A
Cuts at: 96 2880 96
Size: 2784 6283
BssHII G'CGCG_C
Cuts at: 1925 6039 1925
Size: 4114 4953
BssSI C'ACGA_G
Cuts at: 7127 8139 7127
Size: 1012 8055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9067
BstBI TT'CG_AA
Cuts at: 7871 7871
Size: 9067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9067
BstYI r'GATC_y
Cuts at: 1972 2495 2623 5336 6397 6764 7149 7166 8618
Size: 523 128 2713 1061 367 385 17 1452
Cuts at: 8618 8704 8716 9062 1972
Size: 86 12 346 1977
Fragments arranged by size:
2713 1977 1452 1061 523 385 367 346
128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5942 5942
Size: 9067
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 2504 2735 513
Size: 552 939 108 392 231 6845
Fragments arranged by size:
6845 939 552 392 231 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2366 487
Size: 683 56 1140 7188
Fragments arranged by size:
7188 1140 683 56
BtsI GCAGTG_nn'
Cuts at: 2397 3559 6344 6583 7377 7397 2397
Size: 1162 2785 239 794 20 4067
Fragments arranged by size:
4067 2785 1162 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9032
DraI TTT'AAA
Cuts at: 5887 6371 5887
Size: 484 8583
DraIII CAC_nnn'GTG
Cuts at: 5312 5312
Size: 9067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8074 809
Size: 507 6758 1802
Fragments arranged by size:
6758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2662 5330
Size: 54 1425 50 275 18 291 541 2668
Cuts at: 5330 6514 7403 9032 8
Size: 1184 889 1629 43
Fragments arranged by size:
2668 1629 1425 1184 889 541 291 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2121 6514 1537
Size: 275 309 4393 4090
Fragments arranged by size:
4393 4090 309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2294 2874 3295 4122 6229 6257 6997 1353
Size: 941 580 421 827 2107 28 740 3423
Fragments arranged by size:
3423 2107 941 827 740 580 421 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2085 7654 8028 8174 387
Size: 1064 138 496 5569 374 146 1280
Fragments arranged by size:
5569 1280 1064 496 374 146 138
EcoICRI GAG'CTC
Cuts at: 3230 5927 3230
Size: 2697 6370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2526 2855 2897 3272 4016 4021
Size: 393 410 484 329 42 375 744 5
Cuts at: 4021 4741 5118 5562 5771 5990 6165 6281 7130
Size: 720 377 444 209 219 175 116 849
Cuts at: 7130 7727 8514 8782 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 849 787 744 720 597 484 444
410 393 377 375 329 268 219 209
175 116 42 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2521 2975 4872 4968 2521
Size: 454 1897 96 6620
Fragments arranged by size:
6620 1897 454 96
EcoRI G'AATT_C
Cuts at: 4477 6376 6754 4477
Size: 1899 378 6790
Fragments arranged by size:
6790 1899 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3727 3727
Size: 9067
FspI TGC'GCA
Cuts at: 7573 7573
Size: 9067
HaeII r_GCGC'y
Cuts at: 1678 5506 8214 1678
Size: 3828 2708 2531
Fragments arranged by size:
3828 2708 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3173 3205 3991 4023 5631
Size: 32 135 32 1601 32 786 32 1608
Cuts at: 5631 5644 5663 5676 6016 6040 6048 6072 7708
Size: 13 19 13 340 24 8 24 1636
Cuts at: 7708 7740 7782 7814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1636 1608 1601 786 340 135 42
32 32 32 32 32 32 24 24
19 13 13 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2211 4906 6411 6657 669
Size: 988 281 273 2695 1505 246 3079
Fragments arranged by size:
3079 2695 1505 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 2362 748
Size: 1614 7453
HpaI GTT'AAC
Cuts at: 6657 6657
Size: 9067
KpnI G_GTAC'C
Cuts at: 6392 6392
Size: 9067
MfeI C'AATT_G
Cuts at: 3395 6666 3395
Size: 3271 5796
MluI A'CGCG_T
Cuts at: 8943 8943
Size: 9067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3648 3960 4326 5228 5597 8181 8365 1305
Size: 2343 312 366 902 369 2584 184 2007
Fragments arranged by size:
2584 2343 2007 902 369 366 312 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5332 10
Size: 54 1425 343 3500 3745
Fragments arranged by size:
3745 3500 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 5300 5746 7025 7384 7543 518
Size: 648 2490 1644 446 1279 359 159 2042
Fragments arranged by size:
2490 2042 1644 1279 648 446 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2035 2067 2126 2132 2587 5695 6317
Size: 414 15 32 59 6 455 3108 622
Cuts at: 6317 6483 7160 8308 8553 1606
Size: 166 677 1148 245 2120
Fragments arranged by size:
3108 2120 1148 677 622 455 414 245
166 59 32 15 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 2735 513
Size: 552 939 731 6845
Fragments arranged by size:
6845 939 731 552
NdeI CA'TA_TG
Cuts at: 387 2470 4249 387
Size: 2083 1779 5205
Fragments arranged by size:
5205 2083 1779
NheI G'CTAG_C
Cuts at: 1052 2138 2270 1052
Size: 1086 132 7849
Fragments arranged by size:
7849 1086 132
NotI GC'GGCC_GC
Cuts at: 2121 6514 2121
Size: 4393 4674
NspI r_CATG'y
Cuts at: 1132 1229 2812 4768 5150 5377 5479 6425 7970
Size: 97 1583 1956 382 227 102 946 1545
Cuts at: 7970 1132
Size: 2229
Fragments arranged by size:
2229 1956 1583 1545 946 382 227 102
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9067
PciI A'CATG_T
Cuts at: 4764 7966 4764
Size: 3202 5865
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2035 2833 4199 1108
Size: 927 798 1366 5976
Fragments arranged by size:
5976 1366 927 798
PfoI T'CCnGG_A
Cuts at: 5134 5134
Size: 9067
PmeI GTTT'AAAC
Cuts at: 6371 6371
Size: 9067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2784 2816 5440 5472 7136 7168 8649 8681 2784
Size: 32 2624 32 1664 32 1481 32 3170
Fragments arranged by size:
3170 2624 1664 1481 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2077 1613
Size: 464 8603
PsiI TTA'TAA
Cuts at: 4413 6637 4413
Size: 2224 6843
PspXI vC'TCGA_Gb
Cuts at: 5439 5439
Size: 9067
PstI C_TGCA'G
Cuts at: 830 2131 3733 3771 5694 6419 830
Size: 1301 1602 38 1923 725 3478
Fragments arranged by size:
3478 1923 1602 1301 725 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9067
PvuII CAG'CTG
Cuts at: 2020 2035 2132 2587 5695 6317 2020
Size: 15 97 455 3108 622 4770
Fragments arranged by size:
4770 3108 622 455 97 15
SacI G_AGCT'C
Cuts at: 3232 5929 3232
Size: 2697 6370
SalI G'TCGA_C
Cuts at: 1936 6409 1936
Size: 4473 4594
SbfI CC_TGCA'GG
Cuts at: 6419 6419
Size: 9067
ScaI AGT'ACT
Cuts at: 3707 7315 9027 3707
Size: 3608 1712 3747
Fragments arranged by size:
3747 3608 1712
SfcI C'TryA_G
Cuts at: 826 1045 2127 3729 3767 4825 4965 5690 6415
Size: 219 1082 1602 38 1058 140 725 725
Cuts at: 6415 7550 8231 8422 826
Size: 1135 681 191 1471
Fragments arranged by size:
1602 1471 1135 1082 1058 725 725 681
219 191 140 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9067
SmaI CCC'GGG
Cuts at: 1872 2103 2145 6394 1872
Size: 231 42 4249 4545
Fragments arranged by size:
4545 4249 231 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 3491 5343 5439 5624 7171
Size: 197 926 804 744 1852 96 185 1547
Cuts at: 7171 8072 8334 820
Size: 901 262 1553
Fragments arranged by size:
1852 1553 1547 926 901 804 744 262
197 185 96
SnaBI TAC'GTA
Cuts at: 493 6858 493
Size: 6365 2702
SpeI A'CTAG_T
Cuts at: 152 4849 152
Size: 4697 4370
SphI G_CATG'C
Cuts at: 1132 2812 5150 5377 6425 1132
Size: 1680 2338 227 1048 3774
Fragments arranged by size:
3774 2338 1680 1048 227
SrfI GCCC'GGGC
Cuts at: 2145 2145
Size: 9067
SspI AAT'ATT
Cuts at: 5 52 4390 6309 6991 5
Size: 47 4338 1919 682 2081
Fragments arranged by size:
4338 2081 1919 682 47
StuI AGG'CCT
Cuts at: 1517 5583 1517
Size: 4066 5001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2225 2735 4449 5635 6506 513
Size: 552 939 221 510 1714 1186 871 3074
Fragments arranged by size:
3074 1714 1186 939 871 552 510 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4754 6115 7094 7111 7264 7449 7894
Size: 1255 3174 1361 979 17 153 185 445
Cuts at: 7894 325
Size: 1498
Fragments arranged by size:
3174 1498 1361 1255 979 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2482 2880 3705
Size: 274 80 33 51 1443 505 398 825
Cuts at: 3705 7313 9025 96
Size: 3608 1712 138
Fragments arranged by size:
3608 1712 1443 825 505 398 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2611 2643 2958 2990 4005 4037 4783
Size: 32 482 32 315 32 1015 32 746
Cuts at: 4783 4815 5116 5148 9027 9059 2097
Size: 32 301 32 3879 32 2105
Fragments arranged by size:
3879 2105 1015 746 482 315 301 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3215 1316
Size: 1899 7168
XbaI T'CTAG_A
Cuts at: 6403 6403
Size: 9067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2389 2389
Size: 9067
XhoI C'TCGA_G
Cuts at: 1943 5439 1943
Size: 3496 5571
XmaI C'CCGG_G
Cuts at: 1870 2101 2143 6392 1870
Size: 231 42 4249 4545
Fragments arranged by size:
4545 4249 231 42
XmnI GAAnn'nnTTC
Cuts at: 3601 3786 4299 5452 7196 3601
Size: 185 513 1153 1744 5472
Fragments arranged by size:
5472 1744 1153 513 185
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6875 276
Size: 53 83 186 936 5341 2468
Fragments arranged by size:
5341 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SwaI