Restriction map for
pFN21AB9455
(Linear) (Six-base) MAPSORT of: KIBB9455.seq Check: 4288 from: 1 to: 4362
KIBB9455.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 963 4362
Size: 963 3399
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 892 1267 2016 2736 3766 4160 4276
Size: 850 42 375 749 720 1030 394 116
Cuts at: 4276 4362
Size: 86
Fragments arranged by size:
1030 850 749 720 394 375 116 86
42
AfeI AGC'GCT
Cuts at: 0 3499 4362
Size: 3499 863
AflII C'TTAA_G
Cuts at: 0 1486 4362
Size: 1486 2876
AflIII A'CryG_T
Cuts at: 0 2759 4362
Size: 2759 1603
AleI CACnn'nnGTG
Cuts at: 0 3295 4362
Size: 3295 1067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3759 4362
Size: 3759 603
AlwNI CAG_nnn'CTG
Cuts at: 0 1057 1141 2537 2613 4362
Size: 1057 84 1396 76 1749
Fragments arranged by size:
1749 1396 1057 84 76
ApaLI G'TGCA_C
Cuts at: 0 3949 4362
Size: 3949 413
ApoI r'AATT_y
Cuts at: 0 166 685 752 2057 2344 2472 2488 3696
Size: 166 519 67 1305 287 128 16 1208
Cuts at: 3696 3847 4362
Size: 151 515
Fragments arranged by size:
1305 1208 519 515 287 166 151 128
67 16
AvaI C'yCGr_G
Cuts at: 0 96 138 3434 4362
Size: 96 42 3296 928
Fragments arranged by size:
3296 928 96 42
BanI G'GyrC_C
Cuts at: 0 2815 4362
Size: 2815 1547
BanII G_rGCy'C
Cuts at: 0 1227 1334 3177 3924 4362
Size: 1227 107 1843 747 438
Fragments arranged by size:
1843 1227 747 438 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 730 1742 4362
Size: 730 1012 2620
Fragments arranged by size:
2620 1012 730
BbvCI CC'TCA_GC
Cuts at: 0 3494 4362
Size: 3494 868
BciVI GTATCCnnnnn_n'
Cuts at: 0 2473 4362
Size: 2473 1889
BclI T'GATC_A
Cuts at: 0 3716 4362
Size: 3716 646
BglI GCCn_nnn'nGGC
Cuts at: 0 109 112 4362
Size: 109 3 4250
Fragments arranged by size:
4250 109 3
BglII A'GATC_T
Cuts at: 0 618 4362
Size: 618 3744
BlpI GC'TnA_GC
Cuts at: 0 151 4362
Size: 151 4211
Bme1580I G_kGCm'C
Cuts at: 0 3587 3953 4362
Size: 3587 366 409
Fragments arranged by size:
3587 409 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 1 3152 4362
Size: 1 3151 1210
Fragments arranged by size:
3151 1210 1
BmtI G_CTAG'C
Cuts at: 0 137 269 4362
Size: 137 132 4093
Fragments arranged by size:
4093 137 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 521 2011 3113 3557 3985 4362
Size: 37 484 1490 1102 444 428 377
Fragments arranged by size:
1490 1102 484 444 428 377 37
Bpu10I CC'TnA_GC
Cuts at: 0 3494 4362
Size: 3494 868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 3323 3640 4362
Size: 727 2596 317 722
Fragments arranged by size:
2596 727 722 317
BsaI GGTCTCn'nnnn_
Cuts at: 0 956 4362
Size: 956 3406
BsaHI Gr'CG_yC
Cuts at: 0 735 4060 4356 4362
Size: 735 3325 296 6
Fragments arranged by size:
3325 735 296 6
BsaWI w'CCGG_w
Cuts at: 0 3408 4362
Size: 3408 954
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 953 983 2000 2030 2613 2643
Size: 92 30 831 30 1017 30 583 30
Cuts at: 2643 2778 2808 3111 3141 4011 4041 4362
Size: 135 30 303 30 870 30 321
Fragments arranged by size:
1017 870 831 583 321 303 135 92
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 617 2981 3194 3326 4362
Size: 80 537 2364 213 132 1036
Fragments arranged by size:
2364 1036 537 213 132 80
BseYI C'CCAG_C
Cuts at: 0 81 1305 3011 4030 4187 4362
Size: 81 1224 1706 1019 157 175
Fragments arranged by size:
1706 1224 1019 175 157 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 160 277 853 991 1283 1339 2849
Size: 31 129 117 576 138 292 56 1510
Cuts at: 2849 4362
Size: 1513
Fragments arranged by size:
1513 1510 576 292 138 129 117 56
31
BsiEI CG_ry'CG
Cuts at: 0 119 4362
Size: 119 4243
BsiHKAI G_wGCw'C
Cuts at: 0 157 1227 3924 3953 4362
Size: 157 1070 2697 29 409
Fragments arranged by size:
2697 1070 409 157 29
BsmI GAATG_Cn'
Cuts at: 0 1725 4300 4362
Size: 1725 2575 62
Fragments arranged by size:
2575 1725 62
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3642 4362
Size: 3642 720
Bsp1286I G_dGCh'C
Cuts at: 0 157 1227 1334 3177 3587 3924 3953 4362
Size: 157 1070 107 1843 410 337 29 409
Fragments arranged by size:
1843 1070 410 409 337 157 107 29
BspHI T'CATG_A
Cuts at: 0 3644 4362
Size: 3644 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 520 963 2379 3586 4362
Size: 520 443 1416 1207 776
Fragments arranged by size:
1416 1207 776 520 443
BsrBI CCG'CTC
Cuts at: 0 2108 4362
Size: 2108 2254
BsrDI GCAATG_nn'
Cuts at: 0 1555 1753 1754 2315 4362
Size: 1555 198 1 561 2047
Fragments arranged by size:
2047 1555 561 198 1
BsrFI r'CCGG_y
Cuts at: 0 146 4362
Size: 146 4216
BsrGI T'GTAC_A
Cuts at: 0 875 4362
Size: 875 3487
BssHII G'CGCG_C
Cuts at: 0 4034 4362
Size: 4034 328
BstYI r'GATC_y
Cuts at: 0 490 618 3331 4362
Size: 490 128 2713 1031
Fragments arranged by size:
2713 1031 490 128
Bsu36I CC'TnA_GG
Cuts at: 0 3937 4362
Size: 3937 425
BtgI C'CryG_G
Cuts at: 0 107 499 730 4362
Size: 107 392 231 3632
Fragments arranged by size:
3632 392 231 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 361 4362
Size: 361 4001
BtsI GCAGTG_nn'
Cuts at: 0 392 1554 4339 4362
Size: 392 1162 2785 23
Fragments arranged by size:
2785 1162 392 23
DraI TTT'AAA
Cuts at: 0 3882 4362
Size: 3882 480
DraIII CAC_nnn'GTG
Cuts at: 0 3307 4362
Size: 3307 1055
EaeI y'GGCC_r
Cuts at: 0 116 657 3325 4362
Size: 116 541 2668 1037
Fragments arranged by size:
2668 1037 541 116
EagI C'GGCC_G
Cuts at: 0 116 4362
Size: 116 4246
EarI CTCTTCn'nnn_
Cuts at: 0 289 869 1290 2117 4224 4252 4362
Size: 289 580 421 827 2107 28 110
Fragments arranged by size:
2107 827 580 421 289 110 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 4362
Size: 80 4282
EcoICRI GAG'CTC
Cuts at: 0 1225 3922 4362
Size: 1225 2697 440
Fragments arranged by size:
2697 1225 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 521 850 892 1267 2011 2016 2736
Size: 37 484 329 42 375 744 5 720
Cuts at: 2736 3113 3557 3766 3985 4160 4276 4362
Size: 377 444 209 219 175 116 86
Fragments arranged by size:
744 720 484 444 377 375 329 219
209 175 116 86 42 37 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 516 970 2867 2963 4362
Size: 516 454 1897 96 1399
Fragments arranged by size:
1897 1399 516 454 96
EcoRI G'AATT_C
Cuts at: 0 2472 4362
Size: 2472 1890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1722 4362
Size: 1722 2640
HaeII r_GCGC'y
Cuts at: 0 3501 4362
Size: 3501 861
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1986 2018 3626 3639 3658 3671
Size: 1168 32 786 32 1608 13 19 13
Cuts at: 3671 4011 4035 4043 4067 4362
Size: 340 24 8 24 295
Fragments arranged by size:
1608 1168 786 340 295 32 32 24
24 19 13 13 8
HincII GTy'rAC
Cuts at: 0 206 2901 4362
Size: 206 2695 1461
Fragments arranged by size:
2695 1461 206
HindIII A'AGCT_T
Cuts at: 0 357 4362
Size: 357 4005
MfeI C'AATT_G
Cuts at: 0 1390 4362
Size: 1390 2972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1643 1955 2321 3223 3592 4362
Size: 1643 312 366 902 369 770
Fragments arranged by size:
1643 902 770 369 366 312
MscI TGG'CCA
Cuts at: 0 3327 4362
Size: 3327 1035
MslI CAynn'nnrTG
Cuts at: 0 1651 3295 3741 4362
Size: 1651 1644 446 621
Fragments arranged by size:
1651 1644 621 446
MspA1I CmG'CkG
Cuts at: 0 15 30 62 121 127 582 3690 4312
Size: 15 15 32 59 6 455 3108 622
Cuts at: 4312 4362
Size: 50
Fragments arranged by size:
3108 622 455 59 50 32 15 15
6
NcoI C'CATG_G
Cuts at: 0 730 4362
Size: 730 3632
NdeI CA'TA_TG
Cuts at: 0 465 2244 4362
Size: 465 1779 2118
Fragments arranged by size:
2118 1779 465
NheI G'CTAG_C
Cuts at: 0 133 265 4362
Size: 133 132 4097
Fragments arranged by size:
4097 133 132
NotI GC'GGCC_GC
Cuts at: 0 116 4362
Size: 116 4246
NspI r_CATG'y
Cuts at: 0 807 2763 3145 3372 3474 4362
Size: 807 1956 382 227 102 888
Fragments arranged by size:
1956 888 807 382 227 102
PciI A'CATG_T
Cuts at: 0 2759 4362
Size: 2759 1603
PflMI CCAn_nnn'nTGG
Cuts at: 0 30 828 2194 4362
Size: 30 798 1366 2168
Fragments arranged by size:
2168 1366 798 30
PfoI T'CCnGG_A
Cuts at: 0 3129 4362
Size: 3129 1233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 779 811 3435 3467 4362
Size: 779 32 2624 32 895
Fragments arranged by size:
2624 895 779 32 32
PshAI GACnn'nnGTC
Cuts at: 0 72 4362
Size: 72 4290
PsiI TTA'TAA
Cuts at: 0 2408 4362
Size: 2408 1954
PspXI vC'TCGA_Gb
Cuts at: 0 3434 4362
Size: 3434 928
PstI C_TGCA'G
Cuts at: 0 126 1728 1766 3689 4362
Size: 126 1602 38 1923 673
Fragments arranged by size:
1923 1602 673 126 38
PvuII CAG'CTG
Cuts at: 0 15 30 127 582 3690 4312 4362
Size: 15 15 97 455 3108 622 50
Fragments arranged by size:
3108 622 455 97 50 15 15
SacI G_AGCT'C
Cuts at: 0 1227 3924 4362
Size: 1227 2697 438
Fragments arranged by size:
2697 1227 438
ScaI AGT'ACT
Cuts at: 0 1702 4362
Size: 1702 2660
SfcI C'TryA_G
Cuts at: 0 122 1724 1762 2820 2960 3685 4362
Size: 122 1602 38 1058 140 725 677
Fragments arranged by size:
1602 1058 725 677 140 122 38
SmaI CCC'GGG
Cuts at: 0 98 140 4362
Size: 98 42 4222
Fragments arranged by size:
4222 98 42
SmlI C'TyrA_G
Cuts at: 0 742 1486 3338 3434 3619 4362
Size: 742 744 1852 96 185 743
Fragments arranged by size:
1852 744 743 742 185 96
SpeI A'CTAG_T
Cuts at: 0 2844 4362
Size: 2844 1518
SphI G_CATG'C
Cuts at: 0 807 3145 3372 4362
Size: 807 2338 227 990
Fragments arranged by size:
2338 990 807 227
SrfI GCCC'GGGC
Cuts at: 0 140 4362
Size: 140 4222
SspI AAT'ATT
Cuts at: 0 2385 4304 4362
Size: 2385 1919 58
Fragments arranged by size:
2385 1919 58
StuI AGG'CCT
Cuts at: 0 3578 4362
Size: 3578 784
StyI C'CwwG_G
Cuts at: 0 220 730 2444 3630 4362
Size: 220 510 1714 1186 732
Fragments arranged by size:
1714 1186 732 510 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2749 4110 4362
Size: 2749 1361 252
Fragments arranged by size:
2749 1361 252
TatI w'GTAC_w
Cuts at: 0 477 875 1700 4362
Size: 477 398 825 2662
Fragments arranged by size:
2662 825 477 398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 606 638 953 985 2000 2032
Size: 92 32 482 32 315 32 1015 32
Cuts at: 2032 2778 2810 3111 3143 4362
Size: 746 32 301 32 1219
Fragments arranged by size:
1219 1015 746 482 315 301 92 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1210 4362
Size: 1210 3152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 4362
Size: 384 3978
XhoI C'TCGA_G
Cuts at: 0 3434 4362
Size: 3434 928
XmaI C'CCGG_G
Cuts at: 0 96 138 4362
Size: 96 42 4224
Fragments arranged by size:
4224 96 42
XmnI GAAnn'nnTTC
Cuts at: 0 1596 1781 2294 3447 4362
Size: 1596 185 513 1153 915
Fragments arranged by size:
1596 1153 915 513 185
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PciI PflMI PfoI PpiI PshAI
PsiI PspXI PstI PvuII SacI ScaI SfcI SmaI
SmlI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BfrBI BmgBI BplI BsaAI BsaBI BsiWI BspEI BssSI
BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DrdI
EcoO109I EcoRV FseI FspI FspAI HpaI KasI KpnI
MluI NaeI NarI NgoMIV NruI NsiI PacI PasI
PmeI PmlI PpuMI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SwaI XbaI ZraI