Restriction map for
pFN21AE6296
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI BssHII Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FbaI FokI
FseI HaeII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MroI MseI
MslI MunI NarI NdeI NheI
NlaIV NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 52 GACGCC G[GA]^CG[CT]C
AlwI 348 GGATC GGATC(4/5)
Asp700I 150 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 82 GAGCTC G[AT]GC[AT]^C
AviII 467 TGCGCA TGC^GCA
BbvI 216 GCTGC GCAGC(8/12)
BglI 490 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 454 TACGTG [CT]AC^GT[GA]
BsaHI 52 GACGCC G[GA]^CG[CT]C
BsgI 415 CTGCAC GTGCAG(16/14)
BsiEI 531 CGGCCG CG[GA][CT]^CG
BsiHKAI 82 GAGCTC G[AT]GC[AT]^C
BsmAI 544 GAGAC GTCTC(1/5)
BsmFI 131 GGGAC GGGAC(10/14)
BsrBI 285 GAGCGG CCGCTC(-3/-3)
BsrFI 490 GCCGGC [GA]^CCGG[CT]
BstAPI 219 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 348 GGATCT [GA]^GATC[CT]
Cfr10I 490 GCCGGC [GA]^CCGG[CT]
Cfr13I 141 GGCCC G^G[ACGT]CC
DdeI 500 CTGAG C^T[ACGT]AG
DpnII 349 GATC ^GATC
EagI 531 CGGCCG C^GGCCG
EclXI 531 CGGCCG C^GGCCG
Eco52I 531 CGGCCG C^GGCCG
EcoRV 253 GATATC GAT^ATC
FauI 287 GCGGG CCCGC(4/6)
FspI 467 TGCGCA TGC^GCA
Hin1I 52 GACGCC G[GA]^CG[CT]C
HincII 331 GTCAAC GT[CT]^[GA]AC
HindII 331 GTCAAC GT[CT]^[GA]AC
HinfI 304 GAGTC G^A[ACGT]TC
HphI 521 TCACC GGTGA(8/7)
MboI 349 GATC ^GATC
MflI 348 GGATCT [GA]^GATC[CT]
NaeI 490 GCCGGC GCC^GGC
NcoI 299 CCATGG C^CATGG
NdeII 349 GATC ^GATC
NgoMIV 490 GCCGGC G^CCGGC
NotI 530 GCGGCCGC GC^GGCCGC
NspI 195 GCATGC [GA]CATG^[CT]
PflMI 393 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 304 GAGTC GAGTC(4/5)
PstI 217 CTGCAG CTGCA^G
SacI 82 GAGCTC GAGCT^C
Sau3AI 349 GATC ^GATC
Sau96I 141 GGCCC G^G[ACGT]CC
SfcI 217 CTGCAG C^T[GA][CT]AG
SphI 195 GCATGC GCATG^C
TaqI 512 TCGA T^CGA
TseI 216 GCTGC G^C[AT]GC
TthHB8I 512 TCGA T^CGA
Van91I 393 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 434 CCATCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 348 GGATCT [GA]^GATC[CT]
XmnI 150 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 229 CGCG CG^CG
535 CGCG
BanII 82 GAGCTC G[GA]GC[CT]^C
289 GGGCTC
BcnI 129 CCGGG CC^[GC]GG
483 CCCGG
BmyI 82 GAGCTC G[AGT]GC[ACT]^C
289 GGGCTC
Bsp1286I 82 GAGCTC G[AGT]GC[ACT]^C
289 GGGCTC
BssSI 418 CACGAG CACGAG(-5/-1)
292 CTCGTG
BstUI 229 CGCG CG^CG
535 CGCG
DsaI 299 CCATGG C^C[GA][CT]GG
365 CCGTGG
EcoT14I 273 CCAAGG C^C[AT][AT]GG
299 CCATGG
HgaI 52 GACGC GACGC(5/10)
536 GCGTC
MboII 324 GAAGA GAAGA(8/7)
155 TCTTC
MnlI 92 CCTC CCTC(7/6)
213 GAGG
MspA1I 358 CAGCTG C[AC]G^C[GT]G
385 CAGCTG
MvnI 229 CGCG CG^CG
535 CGCG
NciI 129 CCGGG CC^[GC]GG
483 CCCGG
NlaIII 196 CATG CATG^
300 CATG
PvuII 358 CAGCTG CAG^CTG
385 CAGCTG
StyI 273 CCAAGG C^C[AT][AT]GG
299 CCATGG
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI AluI AlwNI BalI
BpmI BsaJI BsiYI BslI BspMI
BssKI BstNI Cac8I CfoI EaeI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MluNI
MscI MspI MvaI MwoI RsaI
ScrFI