(Linear) (Six-base) MAPSORT of: KIEE6296.seq Check: 9316 from: 1 to: 549 KIEE6296.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 12 549 Size: 12 537 AlwNI CAG_nnn'CTG Cuts at: 0 207 225 342 549 Size: 207 18 117 207 Fragments arranged by size: 207 207 117 18 BanII G_rGCy'C Cuts at: 0 86 293 549 Size: 86 207 256 Fragments arranged by size: 256 207 86 BglI GCCn_nnn'nGGC Cuts at: 0 496 549 Size: 496 53 BmgBI CAC'GTC Cuts at: 0 138 549 Size: 138 411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 166 340 421 549 Size: 100 66 174 81 128 Fragments arranged by size: 174 128 100 81 66 BsaAI yAC'GTr Cuts at: 0 456 549 Size: 456 93 BsaHI Gr'CG_yC Cuts at: 0 53 549 Size: 53 496 BseYI C'CCAG_C Cuts at: 0 11 278 383 444 549 Size: 11 267 105 61 105 Fragments arranged by size: 267 105 105 61 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 549 Size: 400 149 BsiEI CG_ry'CG Cuts at: 0 534 549 Size: 534 15 BsiHKAI G_wGCw'C Cuts at: 0 86 549 Size: 86 463 Bsp1286I G_dGCh'C Cuts at: 0 86 293 549 Size: 86 207 256 Fragments arranged by size: 256 207 86 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 12 233 422 473 549 Size: 12 221 189 51 76 Fragments arranged by size: 221 189 76 51 12 BsrBI CCG'CTC Cuts at: 0 287 549 Size: 287 262 BsrFI r'CCGG_y Cuts at: 0 490 549 Size: 490 59 BssSI C'ACGA_G Cuts at: 0 292 418 549 Size: 292 126 131 Fragments arranged by size: 292 131 126 BstAPI GCAn_nnn'nTGC Cuts at: 0 225 549 Size: 225 324 BstYI r'GATC_y Cuts at: 0 348 549 Size: 348 201 BtgI C'CryG_G Cuts at: 0 299 365 549 Size: 299 66 184 Fragments arranged by size: 299 184 66 EaeI y'GGCC_r Cuts at: 0 2 234 296 362 531 549 Size: 2 232 62 66 169 18 Fragments arranged by size: 232 169 66 62 18 2 EagI C'GGCC_G Cuts at: 0 531 549 Size: 531 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 297 549 Size: 297 252 EcoICRI GAG'CTC Cuts at: 0 84 549 Size: 84 465 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 166 340 421 549 Size: 100 66 174 81 128 Fragments arranged by size: 174 128 100 81 66 EcoRV GAT'ATC Cuts at: 0 255 549 Size: 255 294 FspI TGC'GCA Cuts at: 0 469 549 Size: 469 80 HincII GTy'rAC Cuts at: 0 333 549 Size: 333 216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 158 549 Size: 158 391 MscI TGG'CCA Cuts at: 0 4 236 298 549 Size: 4 232 62 251 Fragments arranged by size: 251 232 62 4 MspA1I CmG'CkG Cuts at: 0 360 387 549 Size: 360 27 162 Fragments arranged by size: 360 162 27 NaeI GCC'GGC Cuts at: 0 492 549 Size: 492 57 NcoI C'CATG_G Cuts at: 0 299 549 Size: 299 250 NgoMIV G'CCGG_C Cuts at: 0 490 549 Size: 490 59 NotI GC'GGCC_GC Cuts at: 0 531 549 Size: 531 18 NspI r_CATG'y Cuts at: 0 199 549 Size: 199 350 PasI CC'CwG_GG Cuts at: 0 525 549 Size: 525 24 PflMI CCAn_nnn'nTGG Cuts at: 0 399 549 Size: 399 150 PfoI T'CCnGG_A Cuts at: 0 398 549 Size: 398 151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 493 525 549 Size: 493 32 24 Fragments arranged by size: 493 32 24 PstI C_TGCA'G Cuts at: 0 221 549 Size: 221 328 PvuII CAG'CTG Cuts at: 0 360 387 549 Size: 360 27 162 Fragments arranged by size: 360 162 27 SacI G_AGCT'C Cuts at: 0 86 549 Size: 86 463 SfcI C'TryA_G Cuts at: 0 217 549 Size: 217 332 SphI G_CATG'C Cuts at: 0 199 549 Size: 199 350 StyI C'CwwG_G Cuts at: 0 273 299 549 Size: 273 26 250 Fragments arranged by size: 273 250 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 436 549 Size: 436 113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 441 549 Size: 441 108 XmnI GAAnn'nnTTC Cuts at: 0 154 549 Size: 154 395 Enzymes that do cut: AarI AlwNI BanII BglI BmgBI BpmI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstYI BtgI EaeI EagI EciI EcoICRI Eco57MI EcoRV FspI HincII MmeI MscI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PflMI PfoI PpiI PstI PvuII SacI SfcI SphI StyI TaqII XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoO109I EcoRI FalI FseI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI MslI NarI NdeI NheI NruI NsiI PacI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI