Restriction map for
pFN21AE2788
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaBI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MboI
MflI MluI MroI MslI MspI
MvnI NaeI NarI NciI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
Sau3AI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 745 GTAGAC GT^[AC][GT]AC
AcsI 105 AAATTC [GA]^AATT[CT]
AcyI 720 GGCGTC G[GA]^CG[CT]C
AlwNI 712 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 105 AAATTC [GA]^AATT[CT]
AseI 409 ATTAAT AT^TAAT
AsnI 409 ATTAAT AT^TAAT
BalI 404 TGGCCA TGG^CCA
BbsI 758 GTCTTC GAAGAC(2/6)
BpuAI 758 GTCTTC GAAGAC(2/6)
BsaAI 163 TACGTG [CT]AC^GT[GA]
BsaHI 720 GGCGTC G[GA]^CG[CT]C
BsaJI 491 CCATGG C^C[ACGT][ACGT]GG
BseRI 97 GAGGAG GAGGAG(10/8)
BsmI 204 GCATTC GAATGC(1/-1)
BspMI 75 GCAGGT ACCTGC(4/8)
BsrDI 890 GCAATG GCAATG(2/0)
DsaI 491 CCATGG C^C[GA][CT]GG
EaeI 404 TGGCCA [CT]^GGCC[GA]
EcoT14I 491 CCATGG C^C[AT][AT]GG
HgaI 721 GCGTC GACGC(5/10)
Hin1I 720 GGCGTC G[GA]^CG[CT]C
HincII 837 GTCAAC GT[CT]^[GA]AC
HindII 837 GTCAAC GT[CT]^[GA]AC
HphI 399 GGTGA GGTGA(8/7)
MaeII 164 ACGT A^CGT
MfeI 829 CAATTG C^AATTG
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
MseI 410 TTAA T^TAA
MspA1I 544 CAGCGG C[AC]G^C[GT]G
MunI 829 CAATTG C^AATTG
MwoI 218 GCCAACATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 491 CCATGG C^CATGG
PleI 239 GAGTC GAGTC(4/5)
PshBI 409 ATTAAT AT^TAAT
ScaI 32 AGTACT AGT^ACT
SmlI 28 CTTGAG C^T[CT][GA]AG
StyI 491 CCATGG C^C[AT][AT]GG
Tru9I 410 TTAA T^TAA
XbaI 769 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 819 CCGC CCGC(-3/-1)
546 GCGG
AluI 169 AGCT AG^CT
592 AGCT
BfaI 770 CTAG C^TAG
866 CTAG
BsaI 325 GAGACC GGTCTC(1/5)
787 GAGACC
BspHI 693 TCATGA T^CATGA
734 TCATGA
BssKI 396 CCAGG ^CC[ACGT]GG
639 CCTGG
BstNI 396 CCAGG CC^[AT]GG
639 CCTGG
Cac8I 316 GCCAGC GC[ACGT]^[ACGT]GC
593 GCTTGC
Cfr13I 393 GGGCC G^G[ACGT]CC
457 GGCCC
EarI 195 CTCTTC CTCTTC(1/4)
94 GAAGAG
EcoRII 396 CCAGG ^CC[AT]GG
639 CCTGG
Ksp632I 195 CTCTTC CTCTTC(1/4)
94 GAAGAG
MaeI 770 CTAG C^TAG
866 CTAG
MvaI 396 CCAGG CC^[AT]GG
639 CCTGG
NlaIV 336 GGTTCC GG[ACGT]^[ACGT]CC
392 GGGGCC
PflMI 396 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
711 CCAGATTCTGG
PstI 250 CTGCAG CTGCA^G
293 CTGCAG
RcaI 693 TCATGA T^CATGA
734 TCATGA
Sau96I 393 GGGCC G^G[ACGT]CC
457 GGCCC
ScrFI 396 CCAGG CC^[ACGT]GG
639 CCTGG
SfaNI 313 GATGC GCATC(5/9)
575 GCATC
SfcI 250 CTGCAG C^T[GA][CT]AG
293 CTGCAG
Van91I 396 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
711 CCAGATTCTGG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI BbvI BpmI BsiYI BslI
BsmAI BsrI BstF5I DdeI Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboII MnlI NlaIII RsaI TfiI
TseI Tsp45I Tsp509I TspRI