(Circular) (Six-base) MAPSORT of: pFN21AE2788.seq Check: 4795 from: 1 to: 5608 pFN21AE2788 5608 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2071 2071 Size: 5608 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2751 2951 1937 Size: 814 200 4594 Fragments arranged by size: 4594 814 200 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2492 3671 5055 5323 1632 Size: 555 305 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1179 555 305 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2722 3351 4923 2722 Size: 629 1572 3407 Fragments arranged by size: 3407 1572 629 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2110 2917 3143 3295 5404 1493 Size: 617 807 226 152 2109 1697 Fragments arranged by size: 2109 1697 807 617 226 152 AseI AT'TA_AT Cuts at: 160 1032 2415 4162 160 Size: 872 1383 1747 1606 Fragments arranged by size: 1747 1606 1383 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2929 4384 618 Size: 325 217 627 1142 1455 1842 Fragments arranged by size: 1842 1455 1142 627 325 217 BanII G_rGCy'C Cuts at: 1391 2927 1391 Size: 1536 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2756 928 Size: 971 857 3780 Fragments arranged by size: 3780 971 857 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3799 3833 1903 Size: 34 50 34 1778 34 3678 Fragments arranged by size: 3678 1778 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 3669 4825 1790 Size: 1879 1156 2573 Fragments arranged by size: 2573 1879 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2517 3339 4297 Size: 416 53 83 399 24 1100 822 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1100 958 822 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2666 2810 4268 1239 Size: 838 589 144 1458 2579 Fragments arranged by size: 2579 1458 838 589 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 3733 4598 4896 2054 Size: 1679 865 298 2766 Fragments arranged by size: 2766 1679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2324 2786 4271 882 Size: 617 825 462 1485 2219 Fragments arranged by size: 2219 1485 825 617 462 BsaAI yAC'GTr Cuts at: 493 1204 2170 3399 493 Size: 711 966 1229 2702 Fragments arranged by size: 2702 1229 966 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2726 3415 3797 4432 Size: 53 83 186 936 1193 689 382 635 Cuts at: 4432 275 Size: 1451 Fragments arranged by size: 1451 1193 936 689 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 4677 BseYI C'CCAG_C Cuts at: 2087 4811 5307 2087 Size: 2724 496 2388 Fragments arranged by size: 2724 2388 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5035 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3669 3754 4825 3669 Size: 85 1071 4452 Fragments arranged by size: 4452 1071 85 BsmI GAATG_Cn' Cuts at: 2209 3119 3212 2209 Size: 910 93 4605 Fragments arranged by size: 4605 910 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2927 3669 3754 4825 1391 Size: 399 1137 742 85 1071 2174 Fragments arranged by size: 2174 1137 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 2698 2739 3497 5227 2698 Size: 41 758 1730 3079 Fragments arranged by size: 3079 1730 758 41 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2071 2963 844 Size: 1227 892 3489 Fragments arranged by size: 3489 1227 892 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2902 4103 4277 65 Size: 1183 1654 1201 174 1396 Fragments arranged by size: 1654 1396 1201 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603 Size: 966 367 385 17 1452 86 12 346 Cuts at: 5603 1972 Size: 1977 Fragments arranged by size: 1977 1452 966 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3625 Fragments arranged by size: 3625 1431 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2634 3124 3918 3938 2634 Size: 490 794 20 4304 Fragments arranged by size: 4304 794 490 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2042 2077 529 Size: 35 1478 35 4060 Fragments arranged by size: 4060 1478 35 35 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 3055 3944 Size: 54 1425 50 275 18 579 646 889 Cuts at: 3944 5573 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 646 579 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2206 3538 1353 Size: 741 112 1332 3423 Fragments arranged by size: 3423 1332 741 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2187 2492 2666 2810 3671 4268 Size: 393 445 110 305 174 144 861 597 Cuts at: 4268 5055 5323 1239 Size: 787 268 1524 Fragments arranged by size: 1524 861 787 597 445 393 305 268 174 144 110 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 4755 1678 Size: 3077 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 2799 2831 4249 Size: 32 135 32 851 32 344 32 1418 Cuts at: 4249 4281 4323 4355 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1418 851 344 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2844 2952 3198 669 Size: 988 281 906 108 246 3079 Fragments arranged by size: 3079 988 906 281 246 108 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 2834 3207 2834 Size: 373 5235 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 10 Size: 54 1425 343 579 3207 Fragments arranged by size: 3207 1425 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2551 3024 3701 4849 5094 1606 Size: 945 473 677 1148 245 2120 Fragments arranged by size: 2120 1148 945 677 473 245 NcoI C'CATG_G Cuts at: 513 1065 2496 513 Size: 552 1431 3625 Fragments arranged by size: 3625 1431 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2407 2722 1108 Size: 1299 315 3994 Fragments arranged by size: 3994 1299 315 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 2073 2376 3178 2073 Size: 303 802 4503 Fragments arranged by size: 4503 802 303 PstI C_TGCA'G Cuts at: 830 2259 2302 2960 830 Size: 1429 43 658 3478 Fragments arranged by size: 3478 1429 658 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 2039 3856 5568 2039 Size: 1817 1712 2079 Fragments arranged by size: 2079 1817 1712 SfcI C'TryA_G Cuts at: 826 1045 2255 2298 2956 4091 4772 4963 826 Size: 219 1210 43 658 1135 681 191 1471 Fragments arranged by size: 1471 1210 1135 681 658 219 191 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 3712 4613 4875 820 Size: 197 926 90 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 90 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2496 3047 513 Size: 552 1431 551 3074 Fragments arranged by size: 3074 1431 552 551 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3635 3652 3805 3990 4435 325 Size: 1255 2055 17 153 185 445 1498 Fragments arranged by size: 2055 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2037 2676 3854 Size: 274 80 33 51 1443 60 639 1178 Cuts at: 3854 5566 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1178 639 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2774 2944 2774 Size: 170 5438 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI