Restriction map for
pFN21AE2676
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AocI Aor51HI ApaI AscI Asp718I
AviII AvrII BbrPI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BspMI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NgoMIV NheI NotI NruI
PacI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SbfI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 2744 TCCGGA T^CCGGA
AcyI 89 GGCGCC G[GA]^CG[CT]C
AgeI 4184 ACCGGT A^CCGGT
AhdI 22 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3819 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1951 ATTAAT AT^TAAT
AsnI 1951 ATTAAT AT^TAAT
AspEI 22 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 876 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 56 CTCGAG C^[CT]CG[GA]G
BalI 3288 TGGCCA TGG^CCA
BamHI 3423 GGATCC G^GATCC
BbeI 89 GGCGCC GGCGC^C
BbsI 1632 GAAGAC GAAGAC(2/6)
BbvCI 2434 CCTCAGC CCTCAGC(-5/-2)
BpuAI 1632 GAAGAC GAAGAC(2/6)
BsaHI 89 GGCGCC G[GA]^CG[CT]C
BsaI 1111 GAGACC GGTCTC(1/5)
BseAI 2744 TCCGGA T^CCGGA
BseRI 1967 CTCCTC GAGGAG(10/8)
BsmI 1644 GAATGC GAATGC(1/-1)
BsoBI 56 CTCGAG C^[CT]CG[GA]G
BspDI 3937 ATCGAT AT^CGAT
BspEI 2744 TCCGGA T^CCGGA
BspHI 4233 TCATGA T^CATGA
BsrFI 4184 ACCGGT [GA]^CCGG[CT]
BstAPI 2509 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 198 TTCGAA TT^CGAA
Cfr10I 4184 ACCGGT [GA]^CCGG[CT]
ClaI 3937 ATCGAT AT^CGAT
Eam1105I 22 GACTCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1894 GATATC GAT^ATC
HaeII 89 GGCGCC [GA]GCGC^[CT]
HgaI 2334 GACGC GACGC(5/10)
Hin1I 89 GGCGCC G[GA]^CG[CT]C
HincII 1988 GTCGAC GT[CT]^[GA]AC
HindII 1988 GTCGAC GT[CT]^[GA]AC
KasI 89 GGCGCC G^GCGCC
MluNI 3288 TGGCCA TGG^CCA
MroI 2744 TCCGGA T^CCGGA
MscI 3288 TGGCCA TGG^CCA
NarI 89 GGCGCC GG^CGCC
NcoI 770 CCATGG C^CATGG
NspV 198 TTCGAA TT^CGAA
PaeR7I 56 CTCGAG C^TCGAG
PflFI 876 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4184 ACCGGT A^CCGGT
PpuMI 1482 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1951 ATTAAT AT^TAAT
RcaI 4233 TCATGA T^CATGA
SacI 3217 GAGCTC GAGCT^C
SalI 1988 GTCGAC G^TCGAC
SfoI 89 GGCGCC GGC^GCC
SfuI 198 TTCGAA TT^CGAA
Tth111I 876 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2330 CCAGGACGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 56 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 451 GTAGAC GT^[AC][GT]AC
1988 GTCGAC
AflIII 3465 ACATGT A^C[GA][CT]GT
3853 ACATGT
BanI 3 GGCACC G^G[CT][GA]CC
89 GGCGCC
BcgI 3929 GCAGTAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4355 GCAAACTCTTCG
BsaWI 2744 TCCGGA [AT]^CCGG[AT]
4184 ACCGGT
BspLU11I 3465 ACATGT A^CATGT
3853 ACATGT
BstXI 1528 CCAGTCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2906 CCAAACCACTGG
CfoI 90 GCGC GCG^C
3580 GCGC
DraII 1482 AGGACCT [GA]G^G[ACGT]CC[CT]
3571 AGGGCCT
DsaI 596 CCACGG C^C[GA][CT]GG
770 CCATGG
EaeI 270 TGGCCG [CT]^GGCC[GA]
3288 TGGCCA
EcoO109I 1482 AGGACCT [GA]G^G[ACGT]CC[CT]
3571 AGGGCCT
EcoT22I 2381 ATGCAT ATGCA^T
2953 ATGCAT
HhaI 90 GCGC GCG^C
3580 GCGC
HinP1I 90 GCGC G^CGC
3580 GCGC
HindIII 2014 AAGCTT A^AGCTT
2712 AAGCTT
MspA1I 3589 CAGCTG C[AC]G^C[GT]G
4171 CAGCTG
NdeI 1784 CATATG CA^TATG
3527 CATATG
NsiI 2381 ATGCAT ATGCA^T
2953 ATGCAT
PflMI 809 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2011 CCAAAGCTTGG
PleI 22 GACTC GAGTC(4/5)
727 GAGTC
PvuII 3589 CAGCTG CAG^CTG
4171 CAGCTG
SapI 718 GAAGAGC GCTCTTC(1/4)
4045 GAAGAGC
SexAI 1065 ACCAGGT A^CC[AT]GGT
1571 ACCTGGT
SspI 1912 AATATT AAT^ATT
3868 AATATT
Van91I 809 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2011 CCAAAGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI Asp700I AspHI
AvaII BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PstI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI