Restriction map for
pFN21AE2676
(Circular) (Six-base) MAPSORT of: pFN21AE2676.seq Check: 6712 from: 1 to: 9091
pFN21AE2676 9091 bp
With 182 enzymes: SgfI *
July 27, 2017 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6901 278
Size: 53 83 186 936 5365 2468
Fragments arranged by size:
5365 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 3994 6434 1937
Size: 520 1537 2440 4594
Fragments arranged by size:
4594 2440 1537 520
Acc65I G'GTAC_C
Cuts at: 6412 6412
Size: 9091
AclI AA'CG_TT
Cuts at: 1563 7218 7591 1563
Size: 5655 373 3063
Fragments arranged by size:
5655 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2567 2598 4019 4638 4808 7154 8538 8806
Size: 935 31 1421 619 170 2346 1384 268
Cuts at: 8806 1632
Size: 1917
Fragments arranged by size:
2346 1917 1421 1384 935 619 268 170
31
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8894
AflIII A'CryG_T
Cuts at: 1203 5470 5858 7990 8967 1203
Size: 4267 388 2132 977 1327
Fragments arranged by size:
4267 2132 1327 977 388
AgeI A'CCGG_T
Cuts at: 6189 7922 6189
Size: 1733 7358
AhdI GACnn_n'nnGTC
Cuts at: 2032 7820 2032
Size: 5788 3303
AleI CACnn'nnGTG
Cuts at: 1166 3753 5327 1166
Size: 2587 1574 4930
Fragments arranged by size:
4930 2587 1574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9091
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3009 3041 4316 4348 3009
Size: 32 1275 32 7752
Fragments arranged by size:
7752 1275 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5829 6834 8406 5829
Size: 1005 1572 6514
Fragments arranged by size:
6514 1572 1005
ApaLI G'TGCA_C
Cuts at: 2015 2591 6213 7148 8304 2015
Size: 576 3622 935 1156 2802
Fragments arranged by size:
3622 2802 1156 935 576
ApoI r'AATT_y
Cuts at: 1493 2684 3251 4086 4616 5488 6400 6626 6778
Size: 1191 567 835 530 872 912 226 152
Cuts at: 6778 8887 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1191 912 872 835 567 530
226 152
AseI AT'TA_AT
Cuts at: 160 1032 3957 7645 160
Size: 872 2925 3688 1606
Fragments arranged by size:
3688 2925 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9091
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 6416 1870
Size: 73 118 4355 4545
Fragments arranged by size:
4545 4355 118 73
BamHI G'GATC_C
Cuts at: 5428 6421 6788 5428
Size: 993 367 7731
Fragments arranged by size:
7731 993 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2094 6412 7867 618
Size: 325 217 627 221 86 4318 1455 1842
Fragments arranged by size:
4318 1842 1455 627 325 221 217 86
BanII G_rGCy'C
Cuts at: 1391 2068 2775 5226 5919 6410 1391
Size: 677 707 2451 693 491 4072
Fragments arranged by size:
4072 2451 707 693 677 491
BbeI G_GCGC'C
Cuts at: 2098 2098
Size: 9091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3644 928
Size: 971 1745 6375
Fragments arranged by size:
6375 1745 971
BbvCI CC'TCA_GC
Cuts at: 4440 4440
Size: 9091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5923 5957 6349 6383 7282 7316 1903
Size: 34 3986 34 392 34 899 34 3678
Fragments arranged by size:
3986 3678 899 392 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6983 8193 6983
Size: 1210 7881
BclI T'GATC_A
Cuts at: 1548 2932 5308 5994 1548
Size: 1384 2376 686 4645
Fragments arranged by size:
4645 2376 1384 686
BfrBI ATG'CAT
Cuts at: 4388 4960 4388
Size: 572 8519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7702 136
Size: 107 122 71 631 605 6030 1525
Fragments arranged by size:
6030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9086 9086
Size: 9091
BlpI GC'TnA_GC
Cuts at: 6508 6508
Size: 9091
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2595 6217 7152 8308 1790
Size: 229 576 3622 935 1156 2573
Fragments arranged by size:
3622 2573 1156 935 576 229
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4460 5384 5623
Size: 416 53 83 399 24 3043 924 239
Cuts at: 5623 5651 6114 6822 7780 442
Size: 28 463 708 958 1753
Fragments arranged by size:
3043 1753 958 924 708 463 416 399
239 83 53 28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2623 2819 4545 5538 6239 7751 1239
Size: 1384 196 1726 993 701 1512 2579
Fragments arranged by size:
2579 1726 1512 1384 993 701 196
Bpu10I CC'TnA_GC
Cuts at: 4440 5589 4440
Size: 1149 7942
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3384 5478 6287 7216 8081 8379 3384
Size: 2094 809 929 865 298 4096
Fragments arranged by size:
4096 2094 929 865 809 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3110 7754 882
Size: 617 1611 4644 2219
Fragments arranged by size:
4644 2219 1611 617
BsaAI yAC'GTr
Cuts at: 493 1204 6882 493
Size: 711 5678 2702
Fragments arranged by size:
5678 2702 711
BsaBI GATnn'nnATC
Cuts at: 9085 9085
Size: 9091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2095 6898 7280 7915
Size: 53 83 186 936 562 4803 382 635
Cuts at: 7915 275
Size: 1451
Fragments arranged by size:
4803 1451 936 635 562 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4749 6189 7524 7922 8196 8343 8754 4749
Size: 1440 1335 398 274 147 411 5086
Fragments arranged by size:
5086 1440 1335 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3205 3235 4518 4548 5355
Size: 30 165 30 1802 30 1283 30 807
Cuts at: 5355 5385 5511 5541 1178
Size: 30 126 30 4728
Fragments arranged by size:
4728 1802 1283 807 165 126 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3963 1186
Size: 2777 6314
BseYI C'CCAG_C
Cuts at: 4319 5643 5794 5918 6118 6174 8294 8790 4319
Size: 1324 151 124 200 56 2120 496 4620
Fragments arranged by size:
4620 2120 1324 496 200 151 124 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6541 7302 8330 1540
Size: 275 186 4540 761 1028 2301
Fragments arranged by size:
4540 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 2595 5226 6217 7152 7237 8308 2019
Size: 576 2631 991 935 85 1071 2802
Fragments arranged by size:
2802 2631 1071 991 935 576 85
BsmI GAATG_Cn'
Cuts at: 3655 6602 6695 3655
Size: 2947 93 6051
Fragments arranged by size:
6051 2947 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2068 2595 2775 5226 5919 6217
Size: 399 229 49 527 180 2451 693 298
Cuts at: 6217 6410 7152 7237 8308 1391
Size: 193 742 85 1071 2174
Fragments arranged by size:
2451 2174 1071 742 693 527 399 298
229 193 180 85 49
BspEI T'CCGG_A
Cuts at: 4749 8754 4749
Size: 4005 5086
BspHI T'CATG_A
Cuts at: 6238 6980 8710 6238
Size: 742 1730 6619
Fragments arranged by size:
6619 1730 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6446 844
Size: 5602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6978 1394
Size: 429 5155 3507
Fragments arranged by size:
5155 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2418 2748 3564 4298 5706 7586 7760
Size: 1183 1170 330 816 734 1408 1880 174
Cuts at: 7760 65
Size: 1396
Fragments arranged by size:
1880 1408 1396 1183 1170 816 734 330
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6189 7735 7922 1700
Size: 110 4379 1546 187 2869
Fragments arranged by size:
4379 2869 1546 187 110
BsrGI T'GTAC_A
Cuts at: 96 2701 4197 5100 5467 6168 96
Size: 2605 1496 903 367 701 3019
Fragments arranged by size:
3019 2605 1496 903 701 367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9091
BssSI C'ACGA_G
Cuts at: 7151 8163 7151
Size: 1012 8079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4520 794
Size: 3726 5365
BstBI TT'CG_AA
Cuts at: 2204 7895 2204
Size: 5691 3400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9091
BstXI CCAn_nnnn'nTGG
Cuts at: 3540 4918 3540
Size: 1378 7713
BstYI r'GATC_y
Cuts at: 1972 2497 2558 4695 4711 4746 5428 5649 5670
Size: 525 61 2137 16 35 682 221 21
Cuts at: 5670 6421 6788 7173 7190 8642 8728 8740 9086
Size: 751 367 385 17 1452 86 12 346
Cuts at: 9086 1972
Size: 1977
Fragments arranged by size:
2137 1977 1452 751 682 525 385 367
346 221 86 61 35 21 17 16
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2601 2775 513
Size: 552 939 597 174 6829
Fragments arranged by size:
6829 939 597 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2709 4300 487
Size: 683 56 1483 1591 5278
Fragments arranged by size:
5278 1591 1483 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 3854 5389 5562 6607 7401 7421 2391
Size: 1463 1535 173 1045 794 20 4061
Fragments arranged by size:
4061 1535 1463 1045 794 173 20
ClaI AT'CG_AT
Cuts at: 1554 5943 1554
Size: 4389 4702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3017 3052 3504 3539 529
Size: 35 2453 35 452 35 6081
Fragments arranged by size:
6081 2453 452 35 35 35
DraI TTT'AAA
Cuts at: 6395 6395
Size: 9091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8098 809
Size: 507 6782 1802
Fragments arranged by size:
6782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 5293 6538
Size: 54 1425 50 275 18 445 3018 1245
Cuts at: 6538 7427 9056 8
Size: 889 1629 43
Fragments arranged by size:
3018 1629 1425 1245 889 445 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6538 1537
Size: 275 4726 4090
Fragments arranged by size:
4726 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2718 2781 5242 5339 5763 5808 6045 6371
Size: 1365 63 2461 97 424 45 237 326
Cuts at: 6371 7021 1353
Size: 650 3423
Fragments arranged by size:
3423 2461 1365 650 424 326 237 97
63 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2879 7678 8052 8198 387
Size: 1064 138 1290 4799 374 146 1280
Fragments arranged by size:
4799 1290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5224 5224
Size: 9091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2567 2598 2623 2819 4019 4545 4638
Size: 393 935 31 25 196 1200 526 93
Cuts at: 4638 4808 5538 6239 7154 7751 8538 8806 1239
Size: 170 730 701 915 597 787 268 1524
Fragments arranged by size:
1524 1200 935 915 787 730 701 597
526 393 268 196 170 93 31 25
EcoO109I rG'GnC_Cy
Cuts at: 3488 5577 3488
Size: 2089 7002
EcoRI G'AATT_C
Cuts at: 6400 6778 6400
Size: 378 8713
EcoRV GAT'ATC
Cuts at: 3901 3901
Size: 9091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3754 4668 6155 3754
Size: 914 1487 6690
Fragments arranged by size:
6690 1487 914
FspI TGC'GCA
Cuts at: 7597 7597
Size: 9091
HaeII r_GCGC'y
Cuts at: 1678 2098 8238 1678
Size: 420 6140 2531
Fragments arranged by size:
6140 2531 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2717 2749 5177
Size: 32 135 32 446 32 667 32 2428
Cuts at: 5177 5209 5413 5445 5476 5508 5513 5544 5545
Size: 32 204 32 31 32 5 31 1
Cuts at: 5545 5576 7732 7764 7806 7838 1373
Size: 31 2156 32 42 32 2626
Fragments arranged by size:
2626 2428 2156 667 446 204 135 42
32 32 32 32 32 32 32 32
32 31 31 31 5 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3995 6435 6681 669
Size: 988 281 2057 2440 246 3079
Fragments arranged by size:
3079 2440 2057 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4019 4717 748
Size: 3271 698 5122
Fragments arranged by size:
5122 3271 698
HpaI GTT'AAC
Cuts at: 6681 6681
Size: 9091
KasI G'GCGC_C
Cuts at: 2094 2094
Size: 9091
KpnI G_GTAC'C
Cuts at: 6416 6416
Size: 9091
MfeI C'AATT_G
Cuts at: 6690 6690
Size: 9091
MluI A'CGCG_T
Cuts at: 8967 8967
Size: 9091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3021 4032 4209 5175 8205 8389 1305
Size: 1716 1011 177 966 3030 184 2007
Fragments arranged by size:
3030 2007 1716 1011 966 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5295 10
Size: 54 1425 343 3463 3806
Fragments arranged by size:
3806 3463 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3666 3753 5327 5469 5763 7049 7408
Size: 648 2500 87 1574 142 294 1286 359
Cuts at: 7408 7567 518
Size: 159 2042
Fragments arranged by size:
2500 2042 1574 1286 648 359 294 159
142 87
MspA1I CmG'CkG
Cuts at: 1606 5596 6178 6507 7184 8332 8577 1606
Size: 3990 582 329 677 1148 245 2120
Fragments arranged by size:
3990 2120 1148 677 582 329 245
NarI GG'CG_CC
Cuts at: 2095 2095
Size: 9091
NcoI C'CATG_G
Cuts at: 513 1065 2004 2775 513
Size: 552 939 771 6829
Fragments arranged by size:
6829 939 771 552
NdeI CA'TA_TG
Cuts at: 387 3790 5533 387
Size: 3403 1743 3945
Fragments arranged by size:
3945 3403 1743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9091
NotI GC'GGCC_GC
Cuts at: 6538 6538
Size: 9091
NsiI A_TGCA'T
Cuts at: 4390 4962 4390
Size: 572 8519
NspI r_CATG'y
Cuts at: 1132 1229 4015 5474 5862 6449 7994 1132
Size: 97 2786 1459 388 587 1545 2229
Fragments arranged by size:
2786 2229 1545 1459 587 388 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9091
PciI A'CATG_T
Cuts at: 5470 5858 7990 5470
Size: 388 2132 6571
Fragments arranged by size:
6571 2132 388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2820 4022 1108
Size: 1712 1202 6177
Fragments arranged by size:
6177 1712 1202
PfoI T'CCnGG_A
Cuts at: 2907 4334 2907
Size: 1427 7664
PmeI GTTT'AAAC
Cuts at: 6395 6395
Size: 9091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6094 6126 7160 7192 8673 8705 6094
Size: 32 1034 32 1481 32 6480
Fragments arranged by size:
6480 1481 1034 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3488 3488
Size: 9091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9091
PsiI TTA'TAA
Cuts at: 6661 6661
Size: 9091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3924 3956 3924
Size: 32 9059
PstI C_TGCA'G
Cuts at: 830 2446 3951 5601 6288 6443 830
Size: 1616 1505 1650 687 155 3478
Fragments arranged by size:
3478 1650 1616 1505 687 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9091
PvuII CAG'CTG
Cuts at: 5596 6178 5596
Size: 582 8509
SacI G_AGCT'C
Cuts at: 5226 5226
Size: 9091
SalI G'TCGA_C
Cuts at: 1936 3993 6433 1936
Size: 2057 2440 4594
Fragments arranged by size:
4594 2440 2057
SapI GCTCTTCn'nnn_
Cuts at: 2718 6045 2718
Size: 3327 5764
SbfI CC_TGCA'GG
Cuts at: 6443 6443
Size: 9091
ScaI AGT'ACT
Cuts at: 3359 3942 5633 6026 7339 9051 3359
Size: 583 1691 393 1313 1712 3399
Fragments arranged by size:
3399 1712 1691 1313 583 393
SexAI A'CCwGG_T
Cuts at: 3070 3576 3070
Size: 506 8585
SfcI C'TryA_G
Cuts at: 826 1045 2442 2454 2492 3947 4894 5325 5597
Size: 219 1397 12 38 1455 947 431 272
Cuts at: 5597 6284 6292 6439 7574 8255 8446 826
Size: 687 8 147 1135 681 191 1471
Fragments arranged by size:
1471 1455 1397 1135 947 687 681 431
272 219 191 147 38 12 8
SfoI GGC'GCC
Cuts at: 2096 2096
Size: 9091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9091
SmaI CCC'GGG
Cuts at: 1872 6418 1872
Size: 4546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2061 3399 5493 6302 7195 8096
Size: 197 926 118 1338 2094 809 893 901
Cuts at: 8096 8358 820
Size: 262 1553
Fragments arranged by size:
2094 1553 1338 926 901 893 809 262
197 118
SnaBI TAC'GTA
Cuts at: 493 6882 493
Size: 6389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9091
SphI G_CATG'C
Cuts at: 1132 6449 1132
Size: 5317 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5875 7015 5
Size: 47 3867 1956 1140 2081
Fragments arranged by size:
3867 2081 1956 1140 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2466 2775 2814 4926 6530 513
Size: 552 939 462 309 39 2112 1604 3074
Fragments arranged by size:
3074 2112 1604 939 552 462 309 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4337 5642 5670 5750 6188 7118 7135
Size: 1255 2757 1305 28 80 438 930 17
Cuts at: 7135 7288 7473 7918 325
Size: 153 185 445 1498
Fragments arranged by size:
2757 1498 1305 1255 930 445 438 185
153 80 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2701 2714 3060
Size: 274 80 33 51 1443 724 13 346
Cuts at: 3060 3357 3940 4197 4639 5100 5467 5631 5701
Size: 297 583 257 442 461 367 164 70
Cuts at: 5701 5974 6024 6168 7337 9049 96
Size: 273 50 144 1169 1712 138
Fragments arranged by size:
1712 1443 1169 724 583 461 442 367
346 297 274 273 257 164 144 138
80 70 51 50 33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 5271 5303 5355 5387 5511 5543 9051
Size: 32 2646 32 52 32 124 32 3508
Cuts at: 9051 9083 2593
Size: 32 2601
Fragments arranged by size:
3508 2646 2601 124 52 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2884 1316
Size: 1568 7523
XbaI T'CTAG_A
Cuts at: 6427 6427
Size: 9091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4342 4342
Size: 9091
XhoI C'TCGA_G
Cuts at: 1943 2061 1943
Size: 118 8973
XmaI C'CCGG_G
Cuts at: 1870 6416 1870
Size: 4546 4545
XmnI GAAnn'nnTTC
Cuts at: 2582 4104 4137 5337 7220 2582
Size: 1522 33 1200 1883 4453
Fragments arranged by size:
4453 1883 1522 1200 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6899 276
Size: 53 83 186 936 5365 2468
Fragments arranged by size:
5365 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacII
SanDI SfiI SrfI SwaI