(Linear) (Six-base) MAPSORT of: KIEE2676.seq Check: 3936 from: 1 to: 4386 KIEE2676.orf With 182 enzymes: SgfI * July 27, 2017 10:26 .. AccI GT'mk_AC Cuts at: 0 452 1989 4386 Size: 452 1537 2397 Fragments arranged by size: 2397 1537 452 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 593 2014 2633 2803 4386 Size: 562 31 1421 619 170 1583 Fragments arranged by size: 1583 1421 619 562 170 31 AflIII A'CryG_T Cuts at: 0 3465 3853 4386 Size: 3465 388 533 Fragments arranged by size: 3465 533 388 AgeI A'CCGG_T Cuts at: 0 4184 4386 Size: 4184 202 AhdI GACnn_n'nnGTC Cuts at: 0 27 4386 Size: 27 4359 AleI CACnn'nnGTG Cuts at: 0 1748 3322 4386 Size: 1748 1574 1064 Fragments arranged by size: 1748 1574 1064 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1004 1036 2311 2343 4386 Size: 1004 32 1275 32 2043 Fragments arranged by size: 2043 1275 1004 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 3824 4386 Size: 3824 562 ApaLI G'TGCA_C Cuts at: 0 10 586 4208 4386 Size: 10 576 3622 178 Fragments arranged by size: 3622 576 178 10 ApoI r'AATT_y Cuts at: 0 679 1246 2081 2611 3483 4386 Size: 679 567 835 530 872 903 Fragments arranged by size: 903 872 835 679 567 530 AseI AT'TA_AT Cuts at: 0 1952 4386 Size: 1952 2434 AvaI C'yCGr_G Cuts at: 0 56 4386 Size: 56 4330 BamHI G'GATC_C Cuts at: 0 3423 4386 Size: 3423 963 BanI G'GyrC_C Cuts at: 0 3 89 4386 Size: 3 86 4297 Fragments arranged by size: 4297 86 3 BanII G_rGCy'C Cuts at: 0 63 770 3221 3914 4386 Size: 63 707 2451 693 472 Fragments arranged by size: 2451 707 693 472 63 BbeI G_GCGC'C Cuts at: 0 93 4386 Size: 93 4293 BbsI GAAGACnn'nnnn_ Cuts at: 0 1639 4386 Size: 1639 2747 BbvCI CC'TCA_GC Cuts at: 0 2435 4386 Size: 2435 1951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3918 3952 4344 4378 4386 Size: 3918 34 392 34 8 Fragments arranged by size: 3918 392 34 34 8 BclI T'GATC_A Cuts at: 0 927 3303 3989 4386 Size: 927 2376 686 397 Fragments arranged by size: 2376 927 686 397 BfrBI ATG'CAT Cuts at: 0 2383 2955 4386 Size: 2383 572 1431 Fragments arranged by size: 2383 1431 572 Bme1580I G_kGCm'C Cuts at: 0 14 590 4212 4386 Size: 14 576 3622 174 Fragments arranged by size: 3622 576 174 14 BmrI ACTGGGnnnn_n' Cuts at: 0 2455 3379 3618 3646 4109 4386 Size: 2455 924 239 28 463 277 Fragments arranged by size: 2455 924 463 277 239 28 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 618 814 2540 3533 4234 4386 Size: 618 196 1726 993 701 152 Fragments arranged by size: 1726 993 701 618 196 152 Bpu10I CC'TnA_GC Cuts at: 0 2435 3584 4386 Size: 2435 1149 802 Fragments arranged by size: 2435 1149 802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1379 3473 4282 4386 Size: 1379 2094 809 104 Fragments arranged by size: 2094 1379 809 104 BsaI GGTCTCn'nnnn_ Cuts at: 0 1105 4386 Size: 1105 3281 BsaHI Gr'CG_yC Cuts at: 0 90 4386 Size: 90 4296 BsaWI w'CCGG_w Cuts at: 0 2744 4184 4386 Size: 2744 1440 202 Fragments arranged by size: 2744 1440 202 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1200 1230 2513 2543 3350 3380 3506 3536 Size: 1200 30 1283 30 807 30 126 30 Cuts at: 3536 4386 Size: 850 Fragments arranged by size: 1283 1200 850 807 126 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1958 4386 Size: 1958 2428 BseYI C'CCAG_C Cuts at: 0 2314 3638 3789 3913 4113 4169 4386 Size: 2314 1324 151 124 200 56 217 Fragments arranged by size: 2314 1324 217 200 151 124 56 BsiHKAI G_wGCw'C Cuts at: 0 14 590 3221 4212 4386 Size: 14 576 2631 991 174 Fragments arranged by size: 2631 991 576 174 14 BsmI GAATG_Cn' Cuts at: 0 1650 4386 Size: 1650 2736 Bsp1286I G_dGCh'C Cuts at: 0 14 63 590 770 3221 3914 4212 4386 Size: 14 49 527 180 2451 693 298 174 Fragments arranged by size: 2451 693 527 298 180 174 49 14 BspEI T'CCGG_A Cuts at: 0 2744 4386 Size: 2744 1642 BspHI T'CATG_A Cuts at: 0 4233 4386 Size: 4233 153 BsrDI GCAATG_nn' Cuts at: 0 413 743 1559 2293 3701 4386 Size: 413 330 816 734 1408 685 Fragments arranged by size: 1408 816 734 685 413 330 BsrFI r'CCGG_y Cuts at: 0 4184 4386 Size: 4184 202 BsrGI T'GTAC_A Cuts at: 0 696 2192 3095 3462 4163 4386 Size: 696 1496 903 367 701 223 Fragments arranged by size: 1496 903 701 696 367 223 BstAPI GCAn_nnn'nTGC Cuts at: 0 2515 4386 Size: 2515 1871 BstBI TT'CG_AA Cuts at: 0 199 4386 Size: 199 4187 BstXI CCAn_nnnn'nTGG Cuts at: 0 1535 2913 4386 Size: 1535 1378 1473 Fragments arranged by size: 1535 1473 1378 BstYI r'GATC_y Cuts at: 0 492 553 2690 2706 2741 3423 3644 3665 Size: 492 61 2137 16 35 682 221 21 Cuts at: 3665 4386 Size: 721 Fragments arranged by size: 2137 721 682 492 221 61 35 21 16 BtgI C'CryG_G Cuts at: 0 596 770 4386 Size: 596 174 3616 Fragments arranged by size: 3616 596 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 704 2295 4386 Size: 704 1591 2091 Fragments arranged by size: 2091 1591 704 BtsI GCAGTG_nn' Cuts at: 0 386 1849 3384 3557 4386 Size: 386 1463 1535 173 829 Fragments arranged by size: 1535 1463 829 386 173 ClaI AT'CG_AT Cuts at: 0 3938 4386 Size: 3938 448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1012 1047 1499 1534 4386 Size: 1012 35 452 35 2852 Fragments arranged by size: 2852 1012 452 35 35 EaeI y'GGCC_r Cuts at: 0 270 3288 4386 Size: 270 3018 1098 Fragments arranged by size: 3018 1098 270 EarI CTCTTCn'nnn_ Cuts at: 0 713 776 3237 3334 3758 3803 4040 4366 Size: 713 63 2461 97 424 45 237 326 Cuts at: 4366 4386 Size: 20 Fragments arranged by size: 2461 713 424 326 237 97 63 45 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 874 4386 Size: 874 3512 EcoICRI GAG'CTC Cuts at: 0 3219 4386 Size: 3219 1167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 593 618 814 2014 2540 2633 2803 Size: 562 31 25 196 1200 526 93 170 Cuts at: 2803 3533 4234 4386 Size: 730 701 152 Fragments arranged by size: 1200 730 701 562 526 196 170 152 93 31 25 EcoO109I rG'GnC_Cy Cuts at: 0 1483 3572 4386 Size: 1483 2089 814 Fragments arranged by size: 2089 1483 814 EcoRV GAT'ATC Cuts at: 0 1896 4386 Size: 1896 2490 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1749 2663 4150 4386 Size: 1749 914 1487 236 Fragments arranged by size: 1749 1487 914 236 HaeII r_GCGC'y Cuts at: 0 93 4386 Size: 93 4293 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 712 744 3172 3204 3408 3440 Size: 13 32 667 32 2428 32 204 32 Cuts at: 3440 3471 3503 3508 3539 3540 3571 4386 Size: 31 32 5 31 1 31 815 Fragments arranged by size: 2428 815 667 204 32 32 32 32 32 31 31 31 13 5 1 HincII GTy'rAC Cuts at: 0 1990 4386 Size: 1990 2396 HindIII A'AGCT_T Cuts at: 0 2014 2712 4386 Size: 2014 698 1674 Fragments arranged by size: 2014 1674 698 KasI G'GCGC_C Cuts at: 0 89 4386 Size: 89 4297 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1016 2027 2204 3170 4386 Size: 1016 1011 177 966 1216 Fragments arranged by size: 1216 1016 1011 966 177 MscI TGG'CCA Cuts at: 0 3290 4386 Size: 3290 1096 MslI CAynn'nnrTG Cuts at: 0 1661 1748 3322 3464 3758 4386 Size: 1661 87 1574 142 294 628 Fragments arranged by size: 1661 1574 628 294 142 87 MspA1I CmG'CkG Cuts at: 0 3591 4173 4386 Size: 3591 582 213 Fragments arranged by size: 3591 582 213 NarI GG'CG_CC Cuts at: 0 90 4386 Size: 90 4296 NcoI C'CATG_G Cuts at: 0 770 4386 Size: 770 3616 NdeI CA'TA_TG Cuts at: 0 1785 3528 4386 Size: 1785 1743 858 Fragments arranged by size: 1785 1743 858 NsiI A_TGCA'T Cuts at: 0 2385 2957 4386 Size: 2385 572 1429 Fragments arranged by size: 2385 1429 572 NspI r_CATG'y Cuts at: 0 2010 3469 3857 4386 Size: 2010 1459 388 529 Fragments arranged by size: 2010 1459 529 388 PciI A'CATG_T Cuts at: 0 3465 3853 4386 Size: 3465 388 533 Fragments arranged by size: 3465 533 388 PflMI CCAn_nnn'nTGG Cuts at: 0 815 2017 4386 Size: 815 1202 2369 Fragments arranged by size: 2369 1202 815 PfoI T'CCnGG_A Cuts at: 0 902 2329 4386 Size: 902 1427 2057 Fragments arranged by size: 2057 1427 902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4089 4121 4386 Size: 4089 32 265 Fragments arranged by size: 4089 265 32 PpuMI rG'GwC_Cy Cuts at: 0 1483 4386 Size: 1483 2903 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1919 1951 4386 Size: 1919 32 2435 Fragments arranged by size: 2435 1919 32 PstI C_TGCA'G Cuts at: 0 441 1946 3596 4283 4386 Size: 441 1505 1650 687 103 Fragments arranged by size: 1650 1505 687 441 103 PvuII CAG'CTG Cuts at: 0 3591 4173 4386 Size: 3591 582 213 Fragments arranged by size: 3591 582 213 SacI G_AGCT'C Cuts at: 0 3221 4386 Size: 3221 1165 SalI G'TCGA_C Cuts at: 0 1988 4386 Size: 1988 2398 SapI GCTCTTCn'nnn_ Cuts at: 0 713 4040 4386 Size: 713 3327 346 Fragments arranged by size: 3327 713 346 ScaI AGT'ACT Cuts at: 0 1354 1937 3628 4021 4386 Size: 1354 583 1691 393 365 Fragments arranged by size: 1691 1354 583 393 365 SexAI A'CCwGG_T Cuts at: 0 1065 1571 4386 Size: 1065 506 2815 Fragments arranged by size: 2815 1065 506 SfcI C'TryA_G Cuts at: 0 437 449 487 1942 2889 3320 3592 4279 Size: 437 12 38 1455 947 431 272 687 Cuts at: 4279 4287 4386 Size: 8 99 Fragments arranged by size: 1455 947 687 437 431 272 99 38 12 8 SfoI GGC'GCC Cuts at: 0 91 4386 Size: 91 4295 SmlI C'TyrA_G Cuts at: 0 56 1394 3488 4297 4386 Size: 56 1338 2094 809 89 Fragments arranged by size: 2094 1338 809 89 56 SspI AAT'ATT Cuts at: 0 1914 3870 4386 Size: 1914 1956 516 Fragments arranged by size: 1956 1914 516 StyI C'CwwG_G Cuts at: 0 461 770 809 2921 4386 Size: 461 309 39 2112 1465 Fragments arranged by size: 2112 1465 461 309 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2332 3637 3665 3745 4183 4386 Size: 2332 1305 28 80 438 203 Fragments arranged by size: 2332 1305 438 203 80 28 TatI w'GTAC_w Cuts at: 0 696 709 1055 1352 1935 2192 2634 3095 Size: 696 13 346 297 583 257 442 461 Cuts at: 3095 3462 3626 3696 3969 4019 4163 4386 Size: 367 164 70 273 50 144 223 Fragments arranged by size: 696 583 461 442 367 346 297 273 257 223 164 144 70 50 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 588 620 3266 3298 3350 3382 3506 3538 Size: 588 32 2646 32 52 32 124 32 Cuts at: 3538 4386 Size: 848 Fragments arranged by size: 2646 848 588 124 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 879 4386 Size: 879 3507 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2337 4386 Size: 2337 2049 XhoI C'TCGA_G Cuts at: 0 56 4386 Size: 56 4330 XmnI GAAnn'nnTTC Cuts at: 0 577 2099 2132 3332 4386 Size: 577 1522 33 1200 1054 Fragments arranged by size: 1522 1200 1054 577 33 Enzymes that do cut: AccI AcuI AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PciI PflMI PfoI PpiI PpuMI PsrI PstI PvuII SacI SalI SapI ScaI SexAI SfcI SfoI SmlI SspI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AlfI ApaI AscI AsiSI AvrII BaeI BciVI BglI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspMI BsrBI BssHII BssSI BstEII BstZ17I Bsu36I DraI DraIII DrdI EagI EcoNI EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XmaI ZraI