################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII BalI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I CelII Cfr13I CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FbaI FseI FspI HaeIII HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NciI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfcI SfiI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 59 CGCG CG^CG AcsI 664 GAATTC [GA]^AATT[CT] AcyI 1130 GGCGCC G[GA]^CG[CT]C AfaI 1052 GTAC GT^AC AflIII 908 ACATGT A^C[GA][CT]GT AocI 295 CCTGAGG CC^T[ACGT]AGG ApoI 664 GAATTC [GA]^AATT[CT] AseI 493 ATTAAT AT^TAAT AsnI 493 ATTAAT AT^TAAT AvrII 861 CCTAGG C^CTAGG BamHI 300 GGATCC G^GATCC BanII 291 GAGCCC G[GA]GC[CT]^C BbeI 1130 GGCGCC GGCGC^C BglI 955 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1020 AGATCT A^GATCT BlnI 861 CCTAGG C^CTAGG BsaHI 1130 GGCGCC G[GA]^CG[CT]C BsaWI 11 TCCGGT [AT]^CCGG[AT] BsmAI 1101 GAGAC GTCTC(1/5) BsmFI 874 GTCCC GGGAC(10/14) BspDI 559 ATCGAT AT^CGAT BspLU11I 908 ACATGT A^CATGT BsrBI 76 CCGCTC CCGCTC(-3/-3) BsrFI 30 GCCGGT [GA]^CCGG[CT] BsrI 1108 CCAGT ACTGG(1/-1) BstAPI 27 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 881 TTCGAA TT^CGAA BstUI 59 CGCG CG^CG Bsu36I 295 CCTGAGG CC^T[ACGT]AGG Cfr10I 30 GCCGGT [GA]^CCGG[CT] ClaI 559 ATCGAT AT^CGAT DdeI 296 CTGAG C^T[ACGT]AG DsaI 1037 CCATGG C^C[GA][CT]GG Eco81I 295 CCTGAGG CC^T[ACGT]AGG EcoRI 664 GAATTC G^AATTC Hin1I 1130 GGCGCC G[GA]^CG[CT]C HinfI 1090 GAGTC G^A[ACGT]TC KasI 1130 GGCGCC G^GCGCC MaeII 575 ACGT A^CGT MslI 355 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 59 CGCG CG^CG NarI 1130 GGCGCC GG^CGCC NcoI 1037 CCATGG C^CATGG NspV 881 TTCGAA TT^CGAA PleI 1090 GAGTC GAGTC(4/5) PshBI 493 ATTAAT AT^TAAT RsaI 1052 GTAC GT^AC SexAI 635 ACCTGGT A^CC[AT]GGT SfoI 1130 GGCGCC GGC^GCC SfuI 881 TTCGAA TT^CGAA SphI 485 GCATGC GCATG^C SspI 443 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BanI 33 GGTGCC G^G[CT][GA]CC 1130 GGCGCC BbsI 781 GAAGAC GAAGAC(2/6) 835 GAAGAC BpmI 644 CTCCAG CTGGAG(16/14) 937 CTGGAG BpuAI 781 GAAGAC GAAGAC(2/6) 835 GAAGAC BstYI 300 GGATCC [GA]^GATC[CT] 1020 AGATCT Cac8I 168 GCTGGC GC[ACGT]^[ACGT]GC 485 GCATGC EcoT14I 861 CCTAGG C^C[AT][AT]GG 1037 CCATGG EcoT22I 116 ATGCAT ATGCA^T 596 ATGCAT HaeII 678 AGCGCC [GA]GCGC^[CT] 1130 GGCGCC HphI 520 GGTGA GGTGA(8/7) 588 GGTGA MflI 300 GGATCC [GA]^GATC[CT] 1020 AGATCT NdeI 599 CATATG CA^TATG 727 CATATG NsiI 116 ATGCAT ATGCA^T 596 ATGCAT StyI 861 CCTAGG C^C[AT][AT]GG 1037 CCATGG Tsp45I 521 GTGAC ^GT[GC]AC 857 GTGAC XcmI 566 CCACCTGTAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1037 CCATGGAAGGAGTGG XhoII 300 GGATCC [GA]^GATC[CT] 1020 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI CfoI DpnII EcoRII Fnu4HI FokI HapII HhaI HinP1I HpaII ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI ScrFI SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I