(Circular) (Six-base) MAPSORT of: pFN21AE3178.seq Check: 2598 from: 1 to: 5950 pFN21AE3178 5950 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2214 3177 4013 5397 5665 1632 Size: 416 166 963 836 1384 268 1917 Fragments arranged by size: 1917 1384 963 836 416 268 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 2913 4849 5826 1203 Size: 1710 1936 977 1327 Fragments arranged by size: 1936 1710 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 2669 3259 3485 3637 5746 1493 Size: 1176 590 226 152 2109 1697 Fragments arranged by size: 2109 1697 1176 590 226 152 AseI AT'TA_AT Cuts at: 160 1032 2499 4504 160 Size: 872 1467 2005 1606 Fragments arranged by size: 2005 1606 1467 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 AvrII C'CTAG_G Cuts at: 2866 2866 Size: 5950 BamHI G'GATC_C Cuts at: 2305 3280 3647 2305 Size: 975 367 4608 Fragments arranged by size: 4608 975 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 3135 3271 4726 618 Size: 325 217 627 251 1097 136 1455 1842 Fragments arranged by size: 1842 1455 1097 627 325 251 217 136 BanII G_rGCy'C Cuts at: 1391 2300 3269 1391 Size: 909 969 4072 Fragments arranged by size: 4072 969 909 BbeI G_GCGC'C Cuts at: 3139 3139 Size: 5950 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2793 2847 928 Size: 971 894 54 4031 Fragments arranged by size: 4031 971 894 54 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4141 4175 1903 Size: 34 50 34 2120 34 3678 Fragments arranged by size: 3678 2120 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BfrBI ATG'CAT Cuts at: 2123 2603 2123 Size: 480 5470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2966 4561 136 Size: 107 122 71 631 605 1294 1595 1525 Fragments arranged by size: 1595 1525 1294 631 605 122 107 71 BglII A'GATC_T Cuts at: 3025 5945 3025 Size: 2920 3030 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 2043 2718 4011 5167 1790 Size: 253 675 1293 1156 2573 Fragments arranged by size: 2573 1293 1156 675 253 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2634 2963 4610 1239 Size: 1395 329 1647 2579 Fragments arranged by size: 2579 1647 1395 329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4613 882 Size: 617 3114 2219 Fragments arranged by size: 3114 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3136 3757 4139 4774 Size: 53 83 186 936 1603 621 382 635 Cuts at: 4774 275 Size: 1451 Fragments arranged by size: 1603 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2016 4383 4781 5055 5202 5613 2016 Size: 2367 398 274 147 411 2353 Fragments arranged by size: 2367 2353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5725 Fragments arranged by size: 5725 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5950 BseYI C'CCAG_C Cuts at: 2936 3179 5153 5649 2936 Size: 243 1974 496 3237 Fragments arranged by size: 3237 1974 496 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5377 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4011 4096 5167 4011 Size: 85 1071 4794 Fragments arranged by size: 4794 1071 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2300 2718 3269 4011 4096 5167 Size: 399 253 257 418 551 742 85 1071 Cuts at: 5167 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 551 418 399 257 253 85 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2083 3837 1394 Size: 429 260 1754 3507 Fragments arranged by size: 3507 1754 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 4594 4781 1700 Size: 110 225 2559 187 2869 Fragments arranged by size: 2869 2559 225 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 2038 794 Size: 1244 4706 BstBI TT'CG_AA Cuts at: 2887 4754 2887 Size: 1867 4083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstYI r'GATC_y Cuts at: 1972 2305 3025 3280 3647 4032 4049 5501 5587 Size: 333 720 255 367 385 17 1452 86 Cuts at: 5587 5599 5945 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 720 385 367 346 333 255 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2301 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 3042 513 Size: 552 1977 3421 Fragments arranged by size: 3421 1977 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3466 4260 4280 3190 Size: 276 794 20 4860 Fragments arranged by size: 4860 794 276 20 ClaI AT'CG_AT Cuts at: 1554 2565 1554 Size: 1011 4939 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2298 2333 529 Size: 35 1734 35 4146 Fragments arranged by size: 4146 1734 35 35 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3397 4286 5915 Size: 54 1425 50 275 18 1567 889 1629 Cuts at: 5915 8 Size: 43 Fragments arranged by size: 1629 1567 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3880 1353 Size: 2527 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2600 4537 4911 5057 387 Size: 1064 138 1011 1937 374 146 1280 Fragments arranged by size: 1937 1280 1064 1011 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2214 2634 2963 3177 4013 4610 Size: 393 416 166 420 329 214 836 597 Cuts at: 4610 5397 5665 1239 Size: 787 268 1524 Fragments arranged by size: 1524 836 787 597 420 416 393 329 268 214 166 EcoRI G'AATT_C Cuts at: 2669 3259 3637 2669 Size: 590 378 4982 Fragments arranged by size: 4982 590 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2216 2216 Size: 5950 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2687 3139 5097 1678 Size: 1009 452 1958 2531 Fragments arranged by size: 2531 1958 1009 452 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373 Size: 32 135 32 3019 32 42 32 2626 Fragments arranged by size: 3019 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KasI G'GCGC_C Cuts at: 3135 3135 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4128 Fragments arranged by size: 4128 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 3908 4267 4426 518 Size: 648 1198 1544 359 159 2042 Fragments arranged by size: 2042 1544 1198 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3366 4043 5191 5436 1606 Size: 1760 677 1148 245 2120 Fragments arranged by size: 2120 1760 1148 677 245 NarI GG'CG_CC Cuts at: 3136 3136 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 3042 513 Size: 552 1977 3421 Fragments arranged by size: 3421 1977 552 NdeI CA'TA_TG Cuts at: 387 2605 2733 387 Size: 2218 128 3604 Fragments arranged by size: 3604 2218 128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NsiI A_TGCA'T Cuts at: 2125 2605 2125 Size: 480 5470 NspI r_CATG'y Cuts at: 1132 1229 2127 2494 2917 3308 4853 1132 Size: 97 898 367 423 391 1545 2229 Fragments arranged by size: 2229 1545 898 423 391 367 97 PasI CC'CwG_GG Cuts at: 1348 3192 1348 Size: 1844 4106 PciI A'CATG_T Cuts at: 2913 4849 2913 Size: 1936 4014 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PstI C_TGCA'G Cuts at: 830 3302 830 Size: 2472 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SexAI A'CCwGG_T Cuts at: 2640 2640 Size: 5950 SfcI C'TryA_G Cuts at: 826 1045 3298 4433 5114 5305 826 Size: 219 2253 1135 681 191 1471 Fragments arranged by size: 2253 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3137 3137 Size: 5950 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 2494 3308 1132 Size: 1362 814 3774 Fragments arranged by size: 3774 1362 814 SspI AAT'ATT Cuts at: 5 52 2450 3874 5 Size: 47 2398 1424 2081 Fragments arranged by size: 2398 2081 1424 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2866 3042 3389 513 Size: 552 1801 176 347 3074 Fragments arranged by size: 3074 1801 552 347 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3977 3994 4147 4332 4777 325 Size: 1255 2397 17 153 185 445 1498 Fragments arranged by size: 2397 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2578 3049 2578 Size: 471 5479 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI