################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoT22I FauI FseI FspI HapII HgaI Hin1I HpaI HpaII KasI KpnI Ksp632I KspI MaeI MfeI MflI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TseI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AfaI 54 GTAC GT^AC AlwNI 740 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 898 CCTCAGG CC^T[ACGT]AGG Aor51HI 439 AGCGCT AGC^GCT Asp700I 451 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 256 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 522 GGACC G^G[AT]CC BanI 990 GGTGCC G^G[CT][GA]CC BclI 720 TGATCA T^GATCA BsaAI 1019 TACGTG [CT]AC^GT[GA] BsaBI 126 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 557 CCATGG C^C[ACGT][ACGT]GG BsrDI 1150 GCAATG GCAATG(2/0) BstAPI 579 GCAACATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 103 GGTTACC G^GT[ACGT]ACC BstPI 103 GGTTACC G^GT[ACGT]ACC Bsu36I 898 CCTCAGG CC^T[ACGT]AGG Cfr13I 522 GGACC G^G[ACGT]CC DraIII 1016 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 557 CCATGG C^C[GA][CT]GG Eco47III 439 AGCGCT AGC^GCT Eco81I 898 CCTCAGG CC^T[ACGT]AGG EcoO65I 103 GGTTACC G^GT[ACGT]ACC EcoRI 1005 GAATTC G^AATTC EcoRV 619 GATATC GAT^ATC EcoT14I 557 CCATGG C^C[AT][AT]GG FbaI 720 TGATCA T^GATCA Fnu4HI 407 GCCGC GC^[ACGT]GC HaeII 439 AGCGCT [GA]GCGC^[CT] HaeIII 1169 GGCC GG^CC HincII 794 GTCAAC GT[CT]^[GA]AC HindII 794 GTCAAC GT[CT]^[GA]AC HindIII 384 AAGCTT A^AGCTT ItaI 407 GCCGC GC^[ACGT]GC MaeII 1020 ACGT A^CGT MamI 126 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 800 CAGCGG C[AC]G^C[GT]G NcoI 557 CCATGG C^CATGG NdeI 583 CATATG CA^TATG NspI 222 GCATGC [GA]CATG^[CT] PflFI 256 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1209 GAGTC GAGTC(4/5) RsaI 54 GTAC GT^AC SacI 419 GAGCTC GAGCT^C Sau96I 522 GGACC G^G[ACGT]CC ScaI 53 AGTACT AGT^ACT SmlI 1122 CTTGAG C^T[CT][GA]AG SphI 222 GCATGC GCATG^C StyI 557 CCATGG C^C[AT][AT]GG TfiI 742 GATTC G^A[AT]TC Tth111I 256 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1029 CCAGTCTCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 451 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 281 CGCG CG^CG 1205 CGCG AlwI 961 GATCC GGATC(4/5) 1155 GGATC AspHI 419 GAGCTC G[AT]GC[AT]^C 1022 GTGCTC BanII 419 GAGCTC G[GA]GC[CT]^C 1125 GAGCCC BbsI 1259 GAAGAC GAAGAC(2/6) 331 GTCTTC BpuAI 1259 GAAGAC GAAGAC(2/6) 331 GTCTTC BsiHKAI 419 GAGCTC G[AT]GC[AT]^C 1022 GTGCTC BsmAI 375 GTCTC GTCTC(1/5) 1032 GTCTC BssKI 100 CCTGG ^CC[ACGT]GG 624 CCAGG BstNI 100 CCTGG CC^[AT]GG 624 CCAGG BstUI 281 CGCG CG^CG 1205 CGCG EcoRII 100 CCTGG ^CC[AT]GG 624 CCAGG HinfI 742 GATTC G^A[ACGT]TC 1209 GAGTC MboII 1259 GAAGA GAAGA(8/7) 332 TCTTC MslI 163 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 555 CACCATGGTG MvaI 100 CCTGG CC^[AT]GG 624 CCAGG MvnI 281 CGCG CG^CG 1205 CGCG PflMI 665 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 964 CCATCATCTGG ScrFI 100 CCTGG CC^[ACGT]GG 624 CCAGG SfaNI 9 GATGC GCATC(5/9) 1147 GATGC TaqI 423 TCGA T^CGA 1236 TCGA TthHB8I 423 TCGA T^CGA 1236 TCGA Van91I 665 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 964 CCATCATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AcsI AluI ApoI BmyI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BstF5I Cac8I CfoI DdeI DpnII FokI HhaI HinP1I HphI MaeIII MboI MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI Tru9I Tsp45I Tsp509I TspRI