Restriction map for
pFN21AE4080
(Circular) (Six-base) MAPSORT of: pFN21AE4080.seq Check: 1849 from: 1 to: 5980
pFN21AE4080 5980 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4043 5427 5695 1632
Size: 2411 1384 268 1917
Fragments arranged by size:
2411 1917 1384 268
AfeI AGC'GCT
Cuts at: 2446 2446
Size: 5980
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 2564 1166
Size: 1398 4582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2750 3723 5295 2750
Size: 973 1572 3435
Fragments arranged by size:
3435 1572 973
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2100 3010 3065 3289 3515 3667 5776 1493
Size: 607 910 55 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 910 607 226 224 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3305 1870
Size: 73 1362 4545
Fragments arranged by size:
4545 1362 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2995 3301 4756 618
Size: 325 217 627 1208 306 1455 1842
Fragments arranged by size:
1842 1455 1208 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2428 3134 3299 1391
Size: 1037 706 165 4072
Fragments arranged by size:
4072 1037 706 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2329 3271 928
Size: 971 430 942 3637
Fragments arranged by size:
3637 971 942 430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 2725 1548
Size: 1177 4803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3028 3711 4669
Size: 416 53 83 399 24 1611 683 958
Cuts at: 4669 442
Size: 1753
Fragments arranged by size:
1753 1611 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3237 3237
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4640 1239
Size: 3401 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3148 4105 4970 5268 3148
Size: 957 865 298 3860
Fragments arranged by size:
3860 957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3026 3771 493
Size: 711 1822 745 2702
Fragments arranged by size:
2702 1822 745 711
BsaBI GATnn'nnATC
Cuts at: 2135 5974 2135
Size: 3839 2141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2625 2892 3006 1186
Size: 1439 267 114 4160
Fragments arranged by size:
4160 1439 267 114
BseYI C'CCAG_C
Cuts at: 5183 5679 5183
Size: 496 5484
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2428 3031 4041 4126 5197 2428
Size: 603 1010 85 1071 3211
Fragments arranged by size:
3211 1071 1010 603 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2428 3031 3134 3299 4041 4126 5197
Size: 399 638 603 103 165 742 85 1071
Cuts at: 5197 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 638 603 399 165 103
85
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3162 4475 4649 65
Size: 1183 1914 1313 174 1396
Fragments arranged by size:
1914 1396 1313 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 794
Size: 1796 4184
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 2108 1184
Size: 924 5056
BstYI r'GATC_y
Cuts at: 1972 3310 3677 4062 4079 5531 5617 5629 5975
Size: 1338 367 385 17 1452 86 12 346
Cuts at: 5975 1972
Size: 1977
Fragments arranged by size:
1977 1452 1338 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2904 2904
Size: 5980
BtgI C'CryG_G
Cuts at: 513 1065 2562 513
Size: 552 1497 3931
Fragments arranged by size:
3931 1497 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2366 3496 4290 4310 2366
Size: 1130 794 20 4036
Fragments arranged by size:
4036 1130 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DraIII CAC_nnn'GTG
Cuts at: 3026 3026
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945
Size: 54 1425 50 275 18 1597 889 1629
Cuts at: 5945 8
Size: 43
Fragments arranged by size:
1629 1597 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3910 1353
Size: 2557 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2469 4567 4941 5087 387
Size: 1064 138 880 2098 374 146 1280
Fragments arranged by size:
2098 1280 1064 880 374 146 138
EcoICRI GAG'CTC
Cuts at: 2426 2426
Size: 5980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4043 4640 5427 5695 1239
Size: 393 2411 597 787 268 1524
Fragments arranged by size:
2411 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3010 3289 3667 3010
Size: 279 378 5323
Fragments arranged by size:
5323 378 279
EcoRV GAT'ATC
Cuts at: 2626 2626
Size: 5980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2679 2679
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2448 5127 1678
Size: 770 2679 2531
Fragments arranged by size:
2679 2531 770
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 2950 2957 2982
Size: 32 135 32 747 32 599 7 25
Cuts at: 2982 2989 4621 4653 4695 4727 1373
Size: 7 1632 32 42 32 2626
Fragments arranged by size:
2626 1632 747 599 135 42 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2801 3324 3570 669
Size: 988 281 863 523 246 3079
Fragments arranged by size:
3079 988 863 523 281 246
HindIII A'AGCT_T
Cuts at: 748 2389 748
Size: 1641 4339
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2248 5094 5278 1305
Size: 943 2846 184 2007
Fragments arranged by size:
2846 2007 943 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2564 3938 4297 4456 518
Size: 648 1006 392 1374 359 159 2042
Fragments arranged by size:
2042 1374 1006 648 392 359 159
MspA1I CmG'CkG
Cuts at: 1606 2807 3396 4073 5221 5466 1606
Size: 1201 589 677 1148 245 2120
Fragments arranged by size:
2120 1201 1148 677 589 245
NcoI C'CATG_G
Cuts at: 513 1065 2562 513
Size: 552 1497 3931
Fragments arranged by size:
3931 1497 552
NdeI CA'TA_TG
Cuts at: 387 2589 387
Size: 2202 3778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2231 3338 4883 1132
Size: 97 1002 1107 1545 2229
Fragments arranged by size:
2229 1545 1107 1002 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2676 2975 1108
Size: 1568 299 4113
Fragments arranged by size:
4113 1568 299
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3084 3550 3084
Size: 466 5514
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SacI G_AGCT'C
Cuts at: 2428 2428
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 2060 4228 5940 2060
Size: 2168 1712 2100
Fragments arranged by size:
2168 2100 1712
SfcI C'TryA_G
Cuts at: 826 1045 3328 4463 5144 5335 826
Size: 219 2283 1135 681 191 1471
Fragments arranged by size:
2283 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3127 4084 4985 5247 820
Size: 197 926 1184 957 901 262 1553
Fragments arranged by size:
1553 1184 957 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 2231 3338 1132
Size: 1099 1107 3774
Fragments arranged by size:
3774 1107 1099
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2562 3419 513
Size: 552 1497 857 3074
Fragments arranged by size:
3074 1497 857 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 4226 5938
Size: 274 80 33 51 1443 81 2168 1712
Cuts at: 5938 96
Size: 138
Fragments arranged by size:
2168 1712 1443 274 138 81 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 2264 1316
Size: 948 5032
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3041 3041
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 2460 4109 2460
Size: 1649 4331
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI