(Linear) (Six-base) MAPSORT of: KIEE4080.seq Check: 6316 from: 1 to: 1275 KIEE4080.orf With 182 enzymes: SgfI * July 3, 2012 18:30 .. AfeI AGC'GCT Cuts at: 0 441 1275 Size: 441 834 AleI CACnn'nnGTG Cuts at: 0 559 1275 Size: 559 716 AlwNI CAG_nnn'CTG Cuts at: 0 745 1275 Size: 745 530 ApoI r'AATT_y Cuts at: 0 95 1005 1060 1275 Size: 95 910 55 215 Fragments arranged by size: 910 215 95 55 BanI G'GyrC_C Cuts at: 0 990 1275 Size: 990 285 BanII G_rGCy'C Cuts at: 0 423 1129 1275 Size: 423 706 146 Fragments arranged by size: 706 423 146 BbsI GAAGACnn'nnnn_ Cuts at: 0 324 1266 1275 Size: 324 942 9 Fragments arranged by size: 942 324 9 BclI T'GATC_A Cuts at: 0 720 1275 Size: 720 555 BmrI ACTGGGnnnn_n' Cuts at: 0 1023 1275 Size: 1023 252 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1232 1275 Size: 1232 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1143 1275 Size: 1143 132 BsaAI yAC'GTr Cuts at: 0 1021 1275 Size: 1021 254 BsaBI GATnn'nnATC Cuts at: 0 130 1275 Size: 130 1145 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 620 887 1001 1275 Size: 620 267 114 274 Fragments arranged by size: 620 274 267 114 BsiHKAI G_wGCw'C Cuts at: 0 423 1026 1275 Size: 423 603 249 Fragments arranged by size: 603 423 249 Bsp1286I G_dGCh'C Cuts at: 0 423 1026 1129 1275 Size: 423 603 103 146 Fragments arranged by size: 603 423 146 103 BsrDI GCAATG_nn' Cuts at: 0 1157 1275 Size: 1157 118 BstAPI GCAn_nnn'nTGC Cuts at: 0 585 1275 Size: 585 690 BstEII G'GTnAC_C Cuts at: 0 103 1275 Size: 103 1172 Bsu36I CC'TnA_GG Cuts at: 0 899 1275 Size: 899 376 BtgI C'CryG_G Cuts at: 0 557 1275 Size: 557 718 BtsI GCAGTG_nn' Cuts at: 0 361 1275 Size: 361 914 DraIII CAC_nnn'GTG Cuts at: 0 1021 1275 Size: 1021 254 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 464 1275 Size: 464 811 EcoICRI GAG'CTC Cuts at: 0 421 1275 Size: 421 854 EcoRI G'AATT_C Cuts at: 0 1005 1275 Size: 1005 270 EcoRV GAT'ATC Cuts at: 0 621 1275 Size: 621 654 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 674 1275 Size: 674 601 HaeII r_GCGC'y Cuts at: 0 443 1275 Size: 443 832 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 314 346 945 952 977 984 1275 Size: 314 32 599 7 25 7 291 Fragments arranged by size: 599 314 291 32 25 7 7 HincII GTy'rAC Cuts at: 0 796 1275 Size: 796 479 HindIII A'AGCT_T Cuts at: 0 384 1275 Size: 384 891 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 243 1275 Size: 243 1032 MslI CAynn'nnrTG Cuts at: 0 167 559 1275 Size: 167 392 716 Fragments arranged by size: 716 392 167 MspA1I CmG'CkG Cuts at: 0 802 1275 Size: 802 473 NcoI C'CATG_G Cuts at: 0 557 1275 Size: 557 718 NdeI CA'TA_TG Cuts at: 0 584 1275 Size: 584 691 NspI r_CATG'y Cuts at: 0 226 1275 Size: 226 1049 PflMI CCAn_nnn'nTGG Cuts at: 0 671 970 1275 Size: 671 299 305 Fragments arranged by size: 671 305 299 PsiI TTA'TAA Cuts at: 0 1079 1275 Size: 1079 196 SacI G_AGCT'C Cuts at: 0 423 1275 Size: 423 852 ScaI AGT'ACT Cuts at: 0 55 1275 Size: 55 1220 SmlI C'TyrA_G Cuts at: 0 1122 1275 Size: 1122 153 SphI G_CATG'C Cuts at: 0 226 1275 Size: 226 1049 StyI C'CwwG_G Cuts at: 0 557 1275 Size: 557 718 TatI w'GTAC_w Cuts at: 0 53 1275 Size: 53 1222 Tth111I GACn'n_nGTC Cuts at: 0 259 1275 Size: 259 1016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1036 1275 Size: 1036 239 XmnI GAAnn'nnTTC Cuts at: 0 455 1275 Size: 455 820 Enzymes that do cut: AfeI AleI AlwNI ApoI BanI BanII BbsI BclI BmrI BplI BpuEI BsaAI BsaBI BseRI BsiHKAI Bsp1286I BsrDI BstAPI BstEII Bsu36I BtgI BtsI DraIII EciI EcoICRI EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI MslI MspA1I NcoI NdeI NspI PflMI PsiI SacI ScaI SmlI SphI StyI TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI FspI FspAI HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI XbaI XhoI XmaI ZraI