##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HinfI       HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TfiI        TseI        Tsp45I      TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI          113  GTAC                 GT^AC
AhdI          194  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          255  AGCT                 AG^CT
AlwNI         191  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         194  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         193  GGACC                G^G[AT]CC
BglI           37  GCCATCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI         146  CCTCGG               C^C[ACGT][ACGT]GG
BsmFI         247  GTCCC                GGGAC(10/14)
BsrI           19  CCAGT                ACTGG(1/-1)
Cfr13I        193  GGACC                G^G[ACGT]CC
DraII         192  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI           76  CGGCCA               [CT]^GGCC[GA]
Eam1105I      194  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      192  AGGACCT              [GA]G^G[ACGT]CC[CT]
HaeIII         77  GGCC                 GG^CC
HincII         89  GTCAAC               GT[CT]^[GA]AC
HindII         89  GTCAAC               GT[CT]^[GA]AC
MseI          227  TTAA                 T^TAA
MwoI           37  GCCATCCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV          67  GGAGCC               GG[ACGT]^[ACGT]CC
PpuMI         192  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          197  CTGCAG               CTGCA^G
RsaI          113  GTAC                 GT^AC
Sau96I        193  GGACC                G^G[ACGT]CC
SfcI          197  CTGCAG               C^T[GA][CT]AG
TaqI           10  TCGA                 T^CGA
Tru9I         227  TTAA                 T^TAA
Tsp509I       168  AATT                 ^AATT
TthHB8I        10  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BseRI         127  GAGGAG               GAGGAG(10/8)
              154  GAGGAG              
BsiYI          79  CCAGGAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              166  CCAATTCCAGG         
BslI           79  CCAGGAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              166  CCAATTCCAGG         
BspMI         195  ACCTGC               ACCTGC(4/8)
               85  GCAGGT              
BstF5I         39  CATCC                GGATG(2/0)
              150  GGATG               
FokI           39  CATCC                GGATG(9/13)
              150  GGATG               
SspI           55  AATATT               AAT^ATT
              223  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BssKI       BstNI       EcoRII      MboII       MnlI        
MvaI        ScrFI