(Circular) (Six-base) MAPSORT of: pFN21AE6538.seq Check: 5532 from: 1 to: 4969 pFN21AE6538 4969 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2779 278 Size: 53 83 186 936 1243 2468 Fragments arranged by size: 2468 1243 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2312 1937 Size: 375 4594 Acc65I G'GTAC_C Cuts at: 2290 2290 Size: 4969 AclI AA'CG_TT Cuts at: 1563 3096 3469 1563 Size: 1533 373 3063 Fragments arranged by size: 3063 1533 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 3032 4416 4684 1632 Size: 513 887 1384 268 1917 Fragments arranged by size: 1917 1384 887 513 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4772 AflIII A'CryG_T Cuts at: 1203 3868 4845 1203 Size: 2665 977 1327 Fragments arranged by size: 2665 1327 977 AgeI A'CCGG_T Cuts at: 3800 3800 Size: 4969 AhdI GACnn_n'nnGTC Cuts at: 2204 3698 2204 Size: 1494 3475 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4969 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4969 AlwNI CAG_nnn'CTG Cuts at: 2201 2712 4284 2201 Size: 511 1572 2886 Fragments arranged by size: 2886 1572 511 ApaLI G'TGCA_C Cuts at: 3026 4182 3026 Size: 1156 3813 ApoI r'AATT_y Cuts at: 1493 2278 2504 2656 4765 1493 Size: 785 226 152 2109 1697 Fragments arranged by size: 2109 1697 785 226 152 AseI AT'TA_AT Cuts at: 160 1032 3523 160 Size: 872 2491 1606 Fragments arranged by size: 2491 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4969 AvaI C'yCGr_G Cuts at: 1870 1943 2294 1870 Size: 73 351 4545 Fragments arranged by size: 4545 351 73 BamHI G'GATC_C Cuts at: 2299 2666 2299 Size: 367 4602 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2290 3745 618 Size: 325 217 627 503 1455 1842 Fragments arranged by size: 1842 1455 627 503 325 217 BanII G_rGCy'C Cuts at: 1391 2288 1391 Size: 897 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3998 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3160 3194 1903 Size: 34 1223 34 3678 Fragments arranged by size: 3678 1223 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2861 4071 2861 Size: 1210 3759 BclI T'GATC_A Cuts at: 1548 1548 Size: 4969 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 3580 136 Size: 107 122 71 631 605 376 1532 1525 Fragments arranged by size: 1532 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 4964 4964 Size: 4969 BlpI GC'TnA_GC Cuts at: 2386 2386 Size: 4969 Bme1580I G_kGCm'C Cuts at: 1790 3030 4186 1790 Size: 1240 1156 2573 Fragments arranged by size: 2573 1240 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4969 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 3658 442 Size: 416 53 83 399 24 1283 958 1753 Fragments arranged by size: 1753 1283 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4969 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3629 1239 Size: 2390 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3094 3959 4257 3094 Size: 865 298 3806 Fragments arranged by size: 3806 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3632 882 Size: 617 2133 2219 Fragments arranged by size: 2219 2133 617 BsaAI yAC'GTr Cuts at: 493 1204 2760 493 Size: 711 1556 2702 Fragments arranged by size: 2702 1556 711 BsaBI GATnn'nnATC Cuts at: 4963 4963 Size: 4969 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2776 3158 3793 275 Size: 53 83 186 936 1243 382 635 1451 Fragments arranged by size: 1451 1243 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3402 3800 4074 4221 4632 3402 Size: 398 274 147 411 3739 Fragments arranged by size: 3739 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4744 Fragments arranged by size: 4744 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2174 1186 Size: 961 27 3981 Fragments arranged by size: 3981 961 27 BseYI C'CCAG_C Cuts at: 4172 4668 4172 Size: 496 4473 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 3180 4208 1540 Size: 275 186 418 761 1028 2301 Fragments arranged by size: 2301 1028 761 418 275 186 BsiHKAI G_wGCw'C Cuts at: 3030 3115 4186 3030 Size: 85 1071 3813 Fragments arranged by size: 3813 1071 85 BsmI GAATG_Cn' Cuts at: 2480 2573 2480 Size: 93 4876 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2288 3030 3115 4186 1391 Size: 399 498 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 85 BspEI T'CCGG_A Cuts at: 4632 4632 Size: 4969 BspHI T'CATG_A Cuts at: 2858 4588 2858 Size: 1730 3239 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2081 2209 2324 844 Size: 1237 128 115 3489 Fragments arranged by size: 3489 1237 128 115 BsrBI CCG'CTC Cuts at: 1394 1823 2856 1394 Size: 429 1033 3507 Fragments arranged by size: 3507 1033 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3464 3638 65 Size: 1183 2216 174 1396 Fragments arranged by size: 2216 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3613 3800 1700 Size: 110 1803 187 2869 Fragments arranged by size: 2869 1803 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4969 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4969 BssSI C'ACGA_G Cuts at: 3029 4041 3029 Size: 1012 3957 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4969 BstBI TT'CG_AA Cuts at: 3773 3773 Size: 4969 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4969 BstYI r'GATC_y Cuts at: 1972 2299 2666 3051 3068 4520 4606 4618 4964 Size: 327 367 385 17 1452 86 12 346 Cuts at: 4964 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 327 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4417 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4230 Fragments arranged by size: 4230 683 56 BtsI GCAGTG_nn' Cuts at: 2485 3279 3299 2485 Size: 794 20 4155 Fragments arranged by size: 4155 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4969 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4934 DraI TTT'AAA Cuts at: 2273 2273 Size: 4969 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3976 809 Size: 507 2660 1802 Fragments arranged by size: 2660 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2081 2416 3305 Size: 54 1425 50 275 18 251 335 889 Cuts at: 3305 4934 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 335 275 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2416 1537 Size: 275 604 4090 Fragments arranged by size: 4090 604 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2899 1353 Size: 1546 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3556 3930 4076 387 Size: 1064 138 1967 374 146 1280 Fragments arranged by size: 1967 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 3032 3629 4416 4684 1239 Size: 393 513 887 597 787 268 1524 Fragments arranged by size: 1524 887 787 597 513 393 268 EcoO109I rG'GnC_Cy Cuts at: 2198 2198 Size: 4969 EcoRI G'AATT_C Cuts at: 2278 2656 2278 Size: 378 4591 FspI TGC'GCA Cuts at: 3475 3475 Size: 4969 HaeII r_GCGC'y Cuts at: 1678 4116 1678 Size: 2438 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 3610 3642 3684 Size: 32 135 32 618 32 1388 32 42 Cuts at: 3684 3716 1373 Size: 32 2626 Fragments arranged by size: 2626 1388 618 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2096 2313 2559 669 Size: 988 281 158 217 246 3079 Fragments arranged by size: 3079 988 281 246 217 158 HindIII A'AGCT_T Cuts at: 748 748 Size: 4969 HpaI GTT'AAC Cuts at: 2559 2559 Size: 4969 KpnI G_GTAC'C Cuts at: 2294 2294 Size: 4969 MfeI C'AATT_G Cuts at: 2568 2568 Size: 4969 MluI A'CGCG_T Cuts at: 4845 4845 Size: 4969 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4083 4267 1305 Size: 2778 184 2007 Fragments arranged by size: 2778 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3147 Fragments arranged by size: 3147 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2927 3286 3445 518 Size: 648 843 918 359 159 2042 Fragments arranged by size: 2042 918 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2385 3062 4210 4455 1606 Size: 779 677 1148 245 2120 Fragments arranged by size: 2120 1148 779 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4417 NdeI CA'TA_TG Cuts at: 387 387 Size: 4969 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4969 NotI GC'GGCC_GC Cuts at: 2416 2416 Size: 4969 NspI r_CATG'y Cuts at: 1132 1229 2327 3872 1132 Size: 97 1098 1545 2229 Fragments arranged by size: 2229 1545 1098 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4969 PciI A'CATG_T Cuts at: 3868 3868 Size: 4969 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4969 PfoI T'CCnGG_A Cuts at: 2176 2194 2176 Size: 18 4951 PmeI GTTT'AAAC Cuts at: 2273 2273 Size: 4969 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3038 3070 4551 4583 3038 Size: 32 1481 32 3424 Fragments arranged by size: 3424 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2198 2198 Size: 4969 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4969 PsiI TTA'TAA Cuts at: 2539 2539 Size: 4969 PstI C_TGCA'G Cuts at: 830 2206 2321 830 Size: 1376 115 3478 Fragments arranged by size: 3478 1376 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4969 SalI G'TCGA_C Cuts at: 1936 2311 1936 Size: 375 4594 SbfI CC_TGCA'GG Cuts at: 2321 2321 Size: 4969 ScaI AGT'ACT Cuts at: 3217 4929 3217 Size: 1712 3257 SfcI C'TryA_G Cuts at: 826 1045 2202 2317 3452 4133 4324 826 Size: 219 1157 115 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 681 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4969 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4969 SmaI CCC'GGG Cuts at: 1872 2296 1872 Size: 424 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3073 3974 4236 820 Size: 197 926 1130 901 262 1553 Fragments arranged by size: 1553 1130 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2760 493 Size: 2267 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4969 SphI G_CATG'C Cuts at: 1132 2327 1132 Size: 1195 3774 SspI AAT'ATT Cuts at: 5 52 2062 2230 2893 5 Size: 47 2010 168 663 2081 Fragments arranged by size: 2081 2010 663 168 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4969 StyI C'CwwG_G Cuts at: 513 1065 2408 513 Size: 552 1343 3074 Fragments arranged by size: 3074 1343 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2996 3013 3166 3351 3796 325 Size: 1255 1416 17 153 185 445 1498 Fragments arranged by size: 1498 1416 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2117 3215 4927 Size: 274 80 33 51 1443 140 1098 1712 Cuts at: 4927 96 Size: 138 Fragments arranged by size: 1712 1443 1098 274 140 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4929 4961 4929 Size: 32 4937 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4969 XbaI T'CTAG_A Cuts at: 2305 2305 Size: 4969 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4969 XmaI C'CCGG_G Cuts at: 1870 2294 1870 Size: 424 4545 XmnI GAAnn'nnTTC Cuts at: 3098 3098 Size: 4969 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2777 276 Size: 53 83 186 936 1243 2468 Fragments arranged by size: 2468 1243 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI