################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI Eam1105I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluI MluNI MscI MseI MunI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 824 TCCGGA T^CCGGA AcsI 115 GAATTC [GA]^AATT[CT] AlwI 207 GATCC GGATC(4/5) AocI 1501 CCTGAGG CC^T[ACGT]AGG ApoI 115 GAATTC [GA]^AATT[CT] AviII 1852 TGCGCA TGC^GCA BbeI 1639 GGCGCC GGCGC^C BbrPI 217 CACGTG CAC^GTG BbvCI 534 GCTGAGG CCTCAGC(-5/-2) BsaAI 217 CACGTG [CT]AC^GT[GA] BsaI 100 GAGACC GGTCTC(1/5) BsaWI 824 TCCGGA [AT]^CCGG[AT] BseAI 824 TCCGGA T^CCGGA BspEI 824 TCCGGA T^CCGGA BssSI 724 CTCGTG CACGAG(-5/-1) Bsu36I 1501 CCTGAGG CC^T[ACGT]AGG DraIII 1075 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1483 GACTTATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1501 CCTGAGG CC^T[ACGT]AGG EcoRI 115 GAATTC G^AATTC FspI 1852 TGCGCA TGC^GCA KasI 1639 GGCGCC G^GCGCC MroI 824 TCCGGA T^CCGGA MslI 1560 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 232 GCCGGC GCC^GGC NarI 1639 GGCGCC GG^CGCC NgoMIV 232 GCCGGC G^CCGGC NotI 1662 GCGGCCGC GC^GGCCGC PflMI 999 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 217 CACGTG CAC^GTG PmlI 217 CACGTG CAC^GTG PshAI 1339 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 324 CCTGCAGG CCTGCA^GG SfoI 1639 GGCGCC GGC^GCC SmlI 1735 CTCAAG C^T[CT][GA]AG Sse8387I 324 CCTGCAGG CCTGCA^GG TfiI 190 GAATC G^A[AT]TC Tsp509I 116 AATT ^AATT Van91I 999 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1058 CCACACCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 780 GACGCC G[GA]^CG[CT]C 1639 GGCGCC Alw44I 1083 GTGCAC G^TGCAC 1183 GTGCAC Aor51HI 452 AGCGCT AGC^GCT 1625 AGCGCT ApaLI 1083 GTGCAC G^TGCAC 1183 GTGCAC BfaI 914 CTAG C^TAG 1915 CTAG BglI 403 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1124 GCCCTTCCGGC BsaHI 780 GACGCC G[GA]^CG[CT]C 1639 GGCGCC BsgI 214 CTGCAC GTGCAG(16/14) 1387 CTGCAC BsmAI 100 GAGAC GTCTC(1/5) 1711 GAGAC BsrBI 796 CCGCTC CCGCTC(-3/-3) 1839 GAGCGG BsrFI 49 ACCGGC [GA]^CCGG[CT] 232 GCCGGC BstAPI 240 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 474 GCAGGAGCTGC Cfr10I 49 ACCGGC [GA]^CCGG[CT] 232 GCCGGC DpnII 207 GATC ^GATC 1620 GATC EagI 446 CGGCCG C^GGCCG 1663 CGGCCG EclXI 446 CGGCCG C^GGCCG 1663 CGGCCG Eco47III 452 AGCGCT AGC^GCT 1625 AGCGCT Eco52I 446 CGGCCG C^GGCCG 1663 CGGCCG EcoT14I 284 CCATGG C^C[AT][AT]GG 1532 CCATGG Hin1I 780 GACGCC G[GA]^CG[CT]C 1639 GGCGCC MaeI 914 CTAG C^TAG 1915 CTAG MboI 207 GATC ^GATC 1620 GATC NcoI 284 CCATGG C^CATGG 1532 CCATGG NdeII 207 GATC ^GATC 1620 GATC PvuII 373 CAGCTG CAG^CTG 1864 CAGCTG SacI 517 GAGCTC GAGCT^C 991 GAGCTC Sau3AI 207 GATC ^GATC 1620 GATC SmaI 194 CCCGGG CCC^GGG 737 CCCGGG StyI 284 CCATGG C^C[AT][AT]GG 1532 CCATGG XmaCI 194 CCCGGG C^CCGGG 737 CCCGGG XmaI 194 CCCGGG C^CCGGG 737 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI RsaI SacII Sau96I ScrFI SfaNI SfcI SspBI TaqI TseI Tsp45I TspRI TthHB8I