Restriction map for
pFN21AE4179
(Circular) (Six-base) MAPSORT of: pFN21AE4179.seq Check: 6234 from: 1 to: 6637
pFN21AE4179 6637 bp
With 182 enzymes: SgfI *
March 28, 2012 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2226 2226
Size: 6637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4447 278
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3980 1937
Size: 2043 4594
Acc65I G'GTAC_C
Cuts at: 3958 3958
Size: 6637
AclI AA'CG_TT
Cuts at: 1563 4764 5137 1563
Size: 3201 373 3063
Fragments arranged by size:
3201 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 4700 6084 6352 1632
Size: 517 2551 1384 268 1917
Fragments arranged by size:
2551 1917 1384 517 268
AfeI AGC'GCT
Cuts at: 2459 3632 2459
Size: 1173 5464
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6440
AflIII A'CryG_T
Cuts at: 1203 5536 6513 1203
Size: 4333 977 1327
Fragments arranged by size:
4333 1327 977
AgeI A'CCGG_T
Cuts at: 5468 5468
Size: 6637
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6637
AlwNI CAG_nnn'CTG
Cuts at: 2068 2251 2485 4380 5952 2068
Size: 183 234 1895 1572 2753
Fragments arranged by size:
2753 1895 1572 234 183
ApaI G_GGCC'C
Cuts at: 2173 2507 3253 3506 2173
Size: 334 746 253 5304
Fragments arranged by size:
5304 746 334 253
ApaLI G'TGCA_C
Cuts at: 3088 3188 4694 5850 3088
Size: 100 1506 1156 3875
Fragments arranged by size:
3875 1506 1156 100
ApoI r'AATT_y
Cuts at: 1493 2120 3946 4172 4324 6433 1493
Size: 627 1826 226 152 2109 1697
Fragments arranged by size:
2109 1826 1697 627 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5191 160
Size: 872 4159 1606
Fragments arranged by size:
4159 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2579 2716 2742 2945 3439 3962
Size: 73 256 380 137 26 203 494 523
Cuts at: 3962 1870
Size: 4545
Fragments arranged by size:
4545 523 494 380 256 203 137 73
26
BamHI G'GATC_C
Cuts at: 3967 4334 3967
Size: 367 6270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 2575 3532 3542 3558
Size: 325 217 627 768 20 957 10 16
Cuts at: 3558 3644 3763 3958 5413 618
Size: 86 119 195 1455 1842
Fragments arranged by size:
1842 1455 957 768 627 325 217 195
119 86 20 16 10
BanII G_rGCy'C
Cuts at: 1391 2173 2355 2507 2526 2628 2723 2837 2946
Size: 782 182 152 19 102 95 114 109
Cuts at: 2946 3000 3131 3253 3391 3506 3615 3956 1391
Size: 54 131 122 138 115 109 341 4072
Fragments arranged by size:
4072 782 341 182 152 138 131 122
115 114 109 109 102 95 54 19
BbeI G_GCGC'C
Cuts at: 3648 3648
Size: 6637
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5666
BbvCI CC'TCA_GC
Cuts at: 2540 2540
Size: 6637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4828 4862 1903
Size: 34 2891 34 3678
Fragments arranged by size:
3678 2891 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4529 5739 4529
Size: 1210 5427
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2414 3135 5248
Size: 107 122 71 631 605 742 721 2113
Cuts at: 5248 136
Size: 1525
Fragments arranged by size:
2113 1525 742 721 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6632 6632
Size: 6637
BlpI GC'TnA_GC
Cuts at: 4054 4054
Size: 6637
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2507 2560 2580 2875 3081 3092 3192
Size: 383 334 53 20 295 206 11 100
Cuts at: 3192 3231 3253 3379 3506 3545 3561 4698 5854
Size: 39 22 126 127 39 16 1137 1156
Cuts at: 5854 1790
Size: 2573
Fragments arranged by size:
2573 1156 1137 383 334 295 206 127
126 100 53 39 39 22 20 16
11
BmgBI CAC'GTC
Cuts at: 1318 2095 2566 1318
Size: 777 471 5389
Fragments arranged by size:
5389 777 471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2561 2603 4368
Size: 416 53 83 399 24 1144 42 1765
Cuts at: 4368 5326 442
Size: 958 1753
Fragments arranged by size:
1765 1753 1144 958 416 399 83 53
42 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2137 2333 2370 2540 2684 3862 5297
Size: 884 14 196 37 170 144 1178 1435
Cuts at: 5297 1239
Size: 2579
Fragments arranged by size:
2579 1435 1178 884 196 170 144 37
14
Bpu10I CC'TnA_GC
Cuts at: 2540 2540
Size: 6637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3725 4762 5627 5925 3725
Size: 1037 865 298 4437
Fragments arranged by size:
4437 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 5300 882
Size: 617 600 3201 2219
Fragments arranged by size:
3201 2219 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 2224 4428 493
Size: 711 1020 2204 2702
Fragments arranged by size:
2702 2204 1020 711
BsaBI GATnn'nnATC
Cuts at: 6631 6631
Size: 6637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2786 3645 4444 4826
Size: 53 83 186 936 1253 859 799 382
Cuts at: 4826 5461 275
Size: 635 1451
Fragments arranged by size:
1451 1253 936 859 799 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2829 5070 5468 5742 5889 6300 2829
Size: 2241 398 274 147 411 3166
Fragments arranged by size:
3166 2241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2975 3005 3707 3737 1178
Size: 30 165 30 1572 30 702 30 4078
Fragments arranged by size:
4078 1572 702 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 2990 2993 2996 2999 3002 3524 3728
Size: 1720 84 3 3 3 3 522 204
Cuts at: 3728 1186
Size: 4095
Fragments arranged by size:
4095 1720 522 204 84 3 3 3
3
BseYI C'CCAG_C
Cuts at: 3073 3371 3730 3931 5840 6336 3073
Size: 298 359 201 1909 496 3374
Fragments arranged by size:
3374 1909 496 359 298 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 3377 1163
Size: 573 468 1173 4423
Fragments arranged by size:
4423 1173 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2454 3671 3685 4087 4848 5876
Size: 275 186 453 1217 14 402 761 1028
Cuts at: 5876 1540
Size: 2301
Fragments arranged by size:
2301 1217 1028 761 453 402 275 186
14
BsiHKAI G_wGCw'C
Cuts at: 2153 2526 3000 3092 3192 4698 4783 5854 2153
Size: 373 474 92 100 1506 85 1071 2936
Fragments arranged by size:
2936 1506 1071 474 373 100 92 85
BsmI GAATG_Cn'
Cuts at: 4148 4241 4148
Size: 93 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2173 2355 2507 2526 2560 2580
Size: 399 363 20 182 152 19 34 20
Cuts at: 2580 2628 2723 2837 2875 2946 3000 3081 3092
Size: 48 95 114 38 71 54 81 11
Cuts at: 3092 3131 3192 3231 3253 3379 3391 3506 3545
Size: 39 61 39 22 126 12 115 39
Cuts at: 3545 3561 3615 3956 4698 4783 5854 1391
Size: 16 54 341 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 341 182 152
126 115 114 95 85 81 71 61
54 54 48 39 39 39 38 34
22 20 20 19 16 12 11
BspEI T'CCGG_A
Cuts at: 2829 6300 2829
Size: 3471 3166
BspHI T'CATG_A
Cuts at: 4526 6256 4526
Size: 1730 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 2323 2337 3354 3505 3992 844
Size: 1382 97 14 1017 151 487 3489
Fragments arranged by size:
3489 1382 1017 487 151 97 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2803 3846 4524 1394
Size: 429 980 1043 678 3507
Fragments arranged by size:
3507 1043 980 678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 2237 5281 5468 1700
Size: 110 244 183 3044 187 2869
Fragments arranged by size:
3044 2869 244 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 2651 3027 3432 96
Size: 2555 376 405 3301
Fragments arranged by size:
3301 2555 405 376
BssHII G'CGCG_C
Cuts at: 1925 2043 2071 2204 2420 1925
Size: 118 28 133 216 6142
Fragments arranged by size:
6142 216 133 118 28
BssSI C'ACGA_G
Cuts at: 2729 4697 5709 2729
Size: 1968 1012 3657
Fragments arranged by size:
3657 1968 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2251 2485 794
Size: 1457 234 4946
Fragments arranged by size:
4946 1457 234
BstBI TT'CG_AA
Cuts at: 5441 5441
Size: 6637
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 3967 4334 4719 4736 6188 6274 6286 6632
Size: 1995 367 385 17 1452 86 12 346
Cuts at: 6632 1972
Size: 1977
Fragments arranged by size:
1995 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3507 3507
Size: 6637
BtgI C'CryG_G
Cuts at: 513 1065 2289 2508 3093 3383 3421 3537 3705
Size: 552 1224 219 585 290 38 116 168
Cuts at: 3705 513
Size: 3445
Fragments arranged by size:
3445 1224 585 552 290 219 168 116
38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2520 487
Size: 683 56 1294 4604
Fragments arranged by size:
4604 1294 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 3068 3145 3576 3867 4153 4947 4967 2268
Size: 800 77 431 291 286 794 20 3938
Fragments arranged by size:
3938 800 794 431 291 286 77 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6602
DraI TTT'AAA
Cuts at: 3941 3941
Size: 6637
DraIII CAC_nnn'GTG
Cuts at: 3085 3085
Size: 6637
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3494 5644 809
Size: 507 2178 2150 1802
Fragments arranged by size:
2178 2150 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2451 3156 3288
Size: 54 1425 50 275 18 621 705 132
Cuts at: 3288 3668 3702 3873 4084 4973 6602 8
Size: 380 34 171 211 889 1629 43
Fragments arranged by size:
1629 1425 889 705 621 380 275 211
171 132 54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2451 3668 4084 1537
Size: 275 639 1217 416 4090
Fragments arranged by size:
4090 1217 639 416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2949 2955 2967 4567 1353
Size: 1596 6 12 1600 3423
Fragments arranged by size:
3423 1600 1596 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3483 5224 5598 5744 387
Size: 1064 138 1894 1741 374 146 1280
Fragments arranged by size:
1894 1741 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 2998 2524
Size: 474 6163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2137 2149 2333 2370 2540 2684
Size: 393 491 14 12 184 37 170 144
Cuts at: 2684 3862 4700 5297 6084 6352 1239
Size: 1178 838 597 787 268 1524
Fragments arranged by size:
1524 1178 838 787 597 491 393 268
184 170 144 37 14 12
EcoO109I rG'GnC_Cy
Cuts at: 2065 2169 2170 2344 2504 2752 2767 3298 3503
Size: 104 1 174 160 248 15 531 205
Cuts at: 3503 2065
Size: 5199
Fragments arranged by size:
5199 531 248 205 174 160 104 15
1
EcoRI G'AATT_C
Cuts at: 2120 3946 4324 2120
Size: 1826 378 4433
Fragments arranged by size:
4433 1826 378
FspI TGC'GCA
Cuts at: 3859 5143 3859
Size: 1284 5353
HaeII r_GCGC'y
Cuts at: 1678 2251 2365 2377 2461 2641 3585 3634 3648
Size: 573 114 12 84 180 944 49 14
Cuts at: 3648 3920 5784 1678
Size: 272 1864 2531
Fragments arranged by size:
2531 1864 944 573 272 180 114 84
49 14 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5278 5310 5352 5384 1373
Size: 32 135 32 3706 32 42 32 2626
Fragments arranged by size:
3706 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3981 4227 669
Size: 988 281 2043 246 3079
Fragments arranged by size:
3079 2043 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6637
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KasI G'GCGC_C
Cuts at: 3644 3644
Size: 6637
KpnI G_GTAC'C
Cuts at: 3962 3962
Size: 6637
MfeI C'AATT_G
Cuts at: 4236 4236
Size: 6637
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2946 3889 5751 5935 1305
Size: 1641 943 1862 184 2007
Fragments arranged by size:
2007 1862 1641 943 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4815
Fragments arranged by size:
4815 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3569 4595 4954 5113 518
Size: 648 2403 1026 359 159 2042
Fragments arranged by size:
2403 2042 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2380 2510 3039 3095 3360 3385 3423 3673
Size: 774 130 529 56 265 25 38 250
Cuts at: 3673 3871 4053 4730 5878 6123 1606
Size: 198 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 774 677 529 265 250 245
198 182 130 56 38 25
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 6637
NarI GG'CG_CC
Cuts at: 3645 3645
Size: 6637
NcoI C'CATG_G
Cuts at: 513 1065 2289 3537 513
Size: 552 1224 1248 3613
Fragments arranged by size:
3613 1248 1224 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6637
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 6637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 3668 4084 3668
Size: 416 6221
NspI r_CATG'y
Cuts at: 1132 1229 3995 5540 1132
Size: 97 2766 1545 2229
Fragments arranged by size:
2766 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2347 3834 1348
Size: 999 1487 4151
Fragments arranged by size:
4151 1487 999
PciI A'CATG_T
Cuts at: 5536 5536
Size: 6637
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3010 1108
Size: 1902 4735
PfoI T'CCnGG_A
Cuts at: 2517 3483 2517
Size: 966 5671
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PmlI CAC'GTG
Cuts at: 2224 2224
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 6219 6251 4706
Size: 32 1481 32 5092
Fragments arranged by size:
5092 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3348 1613
Size: 1735 4902
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PspOMI G'GGCC_C
Cuts at: 2169 2503 3249 3502 2169
Size: 334 746 253 5304
Fragments arranged by size:
5304 746 334 253
PstI C_TGCA'G
Cuts at: 830 2247 2334 3870 3989 830
Size: 1417 87 1536 119 3478
Fragments arranged by size:
3478 1536 1417 119 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 2380 3871 2380
Size: 1491 5146
SacI G_AGCT'C
Cuts at: 2526 3000 2526
Size: 474 6163
SacII CC_GC'GG
Cuts at: 2511 3096 3386 3424 2511
Size: 585 290 38 5724
Fragments arranged by size:
5724 585 290 38
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SbfI CC_TGCA'GG
Cuts at: 2334 3989 2334
Size: 1655 4982
ScaI AGT'ACT
Cuts at: 4885 6597 4885
Size: 1712 4925
SfcI C'TryA_G
Cuts at: 826 1045 2243 2298 2330 3866 3985 5120 5801
Size: 219 1198 55 32 1536 119 1135 681
Cuts at: 5801 5992 826
Size: 191 1471
Fragments arranged by size:
1536 1471 1198 1135 681 219 191 119
55 32
SfoI GGC'GCC
Cuts at: 3646 3646
Size: 6637
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 2201 2744 3964 1872
Size: 329 543 1220 4545
Fragments arranged by size:
4545 1220 543 329
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3740 4741 5642 5904 820
Size: 197 926 1797 1001 901 262 1553
Fragments arranged by size:
1797 1553 1001 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 3995 1132
Size: 2863 3774
SspI AAT'ATT
Cuts at: 5 52 4561 5
Size: 47 4509 2081
Fragments arranged by size:
4509 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6637
StyI C'CwwG_G
Cuts at: 513 1065 2289 3537 4076 513
Size: 552 1224 1248 539 3074
Fragments arranged by size:
3074 1248 1224 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2204 2543 3017 3107 3293 3550
Size: 1255 543 81 339 474 90 186 257
Cuts at: 3550 4664 4681 4834 5019 5464 325
Size: 1114 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1114 543 474 445 339 257
186 185 153 90 81 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2548 2651 2712
Size: 274 80 33 51 1443 571 103 61
Cuts at: 2712 3027 3432 4883 6595 96
Size: 315 405 1451 1712 138
Fragments arranged by size:
1712 1451 1443 571 405 315 274 138
103 80 61 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 6597 6629 2975
Size: 32 3590 32 2983
Fragments arranged by size:
3590 2983 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3070 3070
Size: 6637
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6637
XmaI C'CCGG_G
Cuts at: 1870 2199 2742 3962 1870
Size: 329 543 1220 4545
Fragments arranged by size:
4545 1220 543 329
XmnI GAAnn'nnTTC
Cuts at: 4766 4766
Size: 6637
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4445 276
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI
PacI PpuMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SrfI SwaI