(Linear) (Six-base) MAPSORT of: KIEE4179.seq Check: 1946 from: 1 to: 1932 KIEE4179.orf With 182 enzymes: SgfI * March 22, 2012 17:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 221 1932 Size: 221 1711 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 1932 Size: 144 1788 AfeI AGC'GCT Cuts at: 0 454 1627 1932 Size: 454 1173 305 Fragments arranged by size: 1173 454 305 AlwNI CAG_nnn'CTG Cuts at: 0 63 246 480 1932 Size: 63 183 234 1452 Fragments arranged by size: 1452 234 183 63 ApaI G_GGCC'C Cuts at: 0 168 502 1248 1501 1932 Size: 168 334 746 253 431 Fragments arranged by size: 746 431 334 253 168 ApaLI G'TGCA_C Cuts at: 0 1083 1183 1932 Size: 1083 100 749 Fragments arranged by size: 1083 749 100 ApoI r'AATT_y Cuts at: 0 115 1932 Size: 115 1817 AvaI C'yCGr_G Cuts at: 0 194 574 711 737 940 1434 1932 Size: 194 380 137 26 203 494 498 Fragments arranged by size: 498 494 380 203 194 137 26 BanI G'GyrC_C Cuts at: 0 550 570 1527 1537 1553 1639 1758 1932 Size: 550 20 957 10 16 86 119 174 Fragments arranged by size: 957 550 174 119 86 20 16 10 BanII G_rGCy'C Cuts at: 0 168 350 502 521 623 718 832 941 Size: 168 182 152 19 102 95 114 109 Cuts at: 941 995 1126 1248 1386 1501 1610 1932 Size: 54 131 122 138 115 109 322 Fragments arranged by size: 322 182 168 152 138 131 122 115 114 109 109 102 95 54 19 BbeI G_GCGC'C Cuts at: 0 1643 1932 Size: 1643 289 BbvCI CC'TCA_GC Cuts at: 0 535 1932 Size: 535 1397 BglI GCCn_nnn'nGGC Cuts at: 0 409 1130 1932 Size: 409 721 802 Fragments arranged by size: 802 721 409 Bme1580I G_kGCm'C Cuts at: 0 168 502 555 575 870 1076 1087 1187 Size: 168 334 53 20 295 206 11 100 Cuts at: 1187 1226 1248 1374 1501 1540 1556 1932 Size: 39 22 126 127 39 16 376 Fragments arranged by size: 376 334 295 206 168 127 126 100 53 39 39 22 20 16 11 BmgBI CAC'GTC Cuts at: 0 90 561 1932 Size: 90 471 1371 Fragments arranged by size: 1371 471 90 BmrI ACTGGGnnnn_n' Cuts at: 0 556 598 1932 Size: 556 42 1334 Fragments arranged by size: 1334 556 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1629 1932 Size: 1629 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 132 328 365 535 679 1857 1932 Size: 118 14 196 37 170 144 1178 75 Fragments arranged by size: 1178 196 170 144 118 75 37 14 Bpu10I CC'TnA_GC Cuts at: 0 535 1932 Size: 535 1397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1720 1932 Size: 1720 212 BsaI GGTCTCn'nnnn_ Cuts at: 0 94 1932 Size: 94 1838 BsaAI yAC'GTr Cuts at: 0 219 1932 Size: 219 1713 BsaHI Gr'CG_yC Cuts at: 0 781 1640 1932 Size: 781 859 292 Fragments arranged by size: 859 781 292 BsaWI w'CCGG_w Cuts at: 0 824 1932 Size: 824 1108 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 970 1000 1702 1732 1932 Size: 970 30 702 30 200 Fragments arranged by size: 970 702 200 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 901 985 988 991 994 997 1519 1723 Size: 901 84 3 3 3 3 522 204 Cuts at: 1723 1932 Size: 209 Fragments arranged by size: 901 522 209 204 84 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1068 1366 1725 1926 1932 Size: 1068 298 359 201 6 Fragments arranged by size: 1068 359 298 201 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 1372 1932 Size: 199 1173 560 Fragments arranged by size: 1173 560 199 BsiEI CG_ry'CG Cuts at: 0 449 1666 1680 1932 Size: 449 1217 14 252 Fragments arranged by size: 1217 449 252 14 BsiHKAI G_wGCw'C Cuts at: 0 148 521 995 1087 1187 1932 Size: 148 373 474 92 100 745 Fragments arranged by size: 745 474 373 148 100 92 Bsp1286I G_dGCh'C Cuts at: 0 148 168 350 502 521 555 575 623 Size: 148 20 182 152 19 34 20 48 Cuts at: 623 718 832 870 941 995 1076 1087 1126 Size: 95 114 38 71 54 81 11 39 Cuts at: 1126 1187 1226 1248 1374 1386 1501 1540 1556 Size: 61 39 22 126 12 115 39 16 Cuts at: 1556 1610 1932 Size: 54 322 Fragments arranged by size: 322 182 152 148 126 115 114 95 81 71 61 54 54 48 39 39 39 38 34 22 20 20 19 16 12 11 BspEI T'CCGG_A Cuts at: 0 824 1932 Size: 824 1108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 221 318 332 1349 1500 1932 Size: 221 97 14 1017 151 432 Fragments arranged by size: 1017 432 221 151 97 14 BsrBI CCG'CTC Cuts at: 0 798 1841 1932 Size: 798 1043 91 Fragments arranged by size: 1043 798 91 BsrFI r'CCGG_y Cuts at: 0 49 232 1932 Size: 49 183 1700 Fragments arranged by size: 1700 183 49 BsrGI T'GTAC_A Cuts at: 0 646 1022 1427 1932 Size: 646 376 405 505 Fragments arranged by size: 646 505 405 376 BssHII G'CGCG_C Cuts at: 0 38 66 199 415 1932 Size: 38 28 133 216 1517 Fragments arranged by size: 1517 216 133 38 28 BssSI C'ACGA_G Cuts at: 0 724 1932 Size: 724 1208 BstAPI GCAn_nnn'nTGC Cuts at: 0 246 480 1932 Size: 246 234 1452 Fragments arranged by size: 1452 246 234 Bsu36I CC'TnA_GG Cuts at: 0 1502 1932 Size: 1502 430 BtgI C'CryG_G Cuts at: 0 284 503 1088 1378 1416 1532 1700 1932 Size: 284 219 585 290 38 116 168 232 Fragments arranged by size: 585 290 284 232 219 168 116 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 515 1932 Size: 515 1417 BtsI GCAGTG_nn' Cuts at: 0 263 1063 1140 1571 1862 1932 Size: 263 800 77 431 291 70 Fragments arranged by size: 800 431 291 263 77 70 DraIII CAC_nnn'GTG Cuts at: 0 1080 1932 Size: 1080 852 DrdI GACnn_nn'nnGTC Cuts at: 0 1489 1932 Size: 1489 443 EaeI y'GGCC_r Cuts at: 0 446 1151 1283 1663 1697 1868 1932 Size: 446 705 132 380 34 171 64 Fragments arranged by size: 705 446 380 171 132 64 34 EagI C'GGCC_G Cuts at: 0 446 1663 1932 Size: 446 1217 269 Fragments arranged by size: 1217 446 269 EarI CTCTTCn'nnn_ Cuts at: 0 944 950 962 1932 Size: 944 6 12 970 Fragments arranged by size: 970 944 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1478 1932 Size: 1478 454 EcoICRI GAG'CTC Cuts at: 0 519 993 1932 Size: 519 474 939 Fragments arranged by size: 939 519 474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 132 144 328 365 535 679 1857 Size: 118 14 12 184 37 170 144 1178 Cuts at: 1857 1932 Size: 75 Fragments arranged by size: 1178 184 170 144 118 75 37 14 12 EcoO109I rG'GnC_Cy Cuts at: 0 60 164 165 339 499 747 762 1293 Size: 60 104 1 174 160 248 15 531 Cuts at: 1293 1498 1932 Size: 205 434 Fragments arranged by size: 531 434 248 205 174 160 104 60 15 1 EcoRI G'AATT_C Cuts at: 0 115 1932 Size: 115 1817 FspI TGC'GCA Cuts at: 0 1854 1932 Size: 1854 78 HaeII r_GCGC'y Cuts at: 0 246 360 372 456 636 1580 1629 1643 Size: 246 114 12 84 180 944 49 14 Cuts at: 1643 1915 1932 Size: 272 17 Fragments arranged by size: 944 272 246 180 114 84 49 17 14 12 KasI G'GCGC_C Cuts at: 0 1639 1932 Size: 1639 293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 941 1884 1932 Size: 941 943 48 Fragments arranged by size: 943 941 48 MslI CAynn'nnrTG Cuts at: 0 1564 1932 Size: 1564 368 MspA1I CmG'CkG Cuts at: 0 375 505 1034 1090 1355 1380 1418 1668 Size: 375 130 529 56 265 25 38 250 Cuts at: 1668 1866 1932 Size: 198 66 Fragments arranged by size: 529 375 265 250 198 130 66 56 38 25 NaeI GCC'GGC Cuts at: 0 234 1932 Size: 234 1698 NarI GG'CG_CC Cuts at: 0 1640 1932 Size: 1640 292 NcoI C'CATG_G Cuts at: 0 284 1532 1932 Size: 284 1248 400 Fragments arranged by size: 1248 400 284 NgoMIV G'CCGG_C Cuts at: 0 232 1932 Size: 232 1700 NotI GC'GGCC_GC Cuts at: 0 1663 1932 Size: 1663 269 PasI CC'CwG_GG Cuts at: 0 342 1829 1932 Size: 342 1487 103 Fragments arranged by size: 1487 342 103 PflMI CCAn_nnn'nTGG Cuts at: 0 1005 1932 Size: 1005 927 PfoI T'CCnGG_A Cuts at: 0 512 1478 1932 Size: 512 966 454 Fragments arranged by size: 966 512 454 PmlI CAC'GTG Cuts at: 0 219 1932 Size: 219 1713 PshAI GACnn'nnGTC Cuts at: 0 1343 1932 Size: 1343 589 PspOMI G'GGCC_C Cuts at: 0 164 498 1244 1497 1932 Size: 164 334 746 253 435 Fragments arranged by size: 746 435 334 253 164 PstI C_TGCA'G Cuts at: 0 242 329 1865 1932 Size: 242 87 1536 67 Fragments arranged by size: 1536 242 87 67 PvuII CAG'CTG Cuts at: 0 375 1866 1932 Size: 375 1491 66 Fragments arranged by size: 1491 375 66 SacI G_AGCT'C Cuts at: 0 521 995 1932 Size: 521 474 937 Fragments arranged by size: 937 521 474 SacII CC_GC'GG Cuts at: 0 506 1091 1381 1419 1932 Size: 506 585 290 38 513 Fragments arranged by size: 585 513 506 290 38 SbfI CC_TGCA'GG Cuts at: 0 329 1932 Size: 329 1603 SfcI C'TryA_G Cuts at: 0 238 293 325 1861 1932 Size: 238 55 32 1536 71 Fragments arranged by size: 1536 238 71 55 32 SfoI GGC'GCC Cuts at: 0 1641 1932 Size: 1641 291 SmaI CCC'GGG Cuts at: 0 196 739 1932 Size: 196 543 1193 Fragments arranged by size: 1193 543 196 SmlI C'TyrA_G Cuts at: 0 1735 1932 Size: 1735 197 StyI C'CwwG_G Cuts at: 0 284 1532 1932 Size: 284 1248 400 Fragments arranged by size: 1248 400 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 118 199 538 1012 1102 1288 1545 1932 Size: 118 81 339 474 90 186 257 387 Fragments arranged by size: 474 387 339 257 186 118 90 81 TatI w'GTAC_w Cuts at: 0 543 646 707 1022 1427 1932 Size: 543 103 61 315 405 505 Fragments arranged by size: 543 505 405 315 103 61 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 970 1002 1932 Size: 970 32 930 Fragments arranged by size: 970 930 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1065 1932 Size: 1065 867 XmaI C'CCGG_G Cuts at: 0 194 737 1932 Size: 194 543 1195 Fragments arranged by size: 1195 543 194 Enzymes that do cut: AarI AcuI AfeI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PasI PflMI PfoI PmlI PshAI PspOMI PstI PvuII SacI SacII SbfI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI EcoNI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NruI NsiI NspI PacI PciI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI