################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FauI FseI FspI HapII HgaI Hin1I HpaI HpaII KasI KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 77 GGTACC G^GTACC AccI 664 GTCTAC GT^[AC][GT]AC AciI 1641 CCGC CCGC(-3/-1) AclI 1609 AACGTT AA^CGTT AflIII 1619 ACATGT A^C[GA][CT]GT Asp718I 77 GGTACC G^GTACC BanI 77 GGTACC G^G[CT][GA]CC BbsI 1924 GAAGAC GAAGAC(2/6) BciVI 1398 GGATAC GTATCC(6/5) BpuAI 1924 GAAGAC GAAGAC(2/6) BsaAI 1119 TACGTG [CT]AC^GT[GA] BsgI 679 CTGCAC GTGCAG(16/14) BsmFI 307 GTCCC GGGAC(10/14) BspLU11I 1619 ACATGT A^CATGT BspMI 677 ACCTGC ACCTGC(4/8) BssSI 1466 CTCGTG CACGAG(-5/-1) BstBI 1578 TTCGAA TT^CGAA BstXI 1844 CCAACATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 1839 GCTTGC GC[ACGT]^[ACGT]GC CfoI 1415 GCGC GCG^C DraI 1893 TTTAAA TTT^AAA EcoNI 569 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 861 GATATC GAT^ATC HaeII 1414 AGCGCC [GA]GCGC^[CT] HhaI 1415 GCGC GCG^C HinP1I 1415 GCGC G^CGC HincII 829 GTTGAC GT[CT]^[GA]AC HindII 829 GTTGAC GT[CT]^[GA]AC HindIII 1837 AAGCTT A^AGCTT KpnI 77 GGTACC GGTAC^C MwoI 1097 GCAAAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 1619 ACATGT [GA]CATG^[CT] NspV 1578 TTCGAA TT^CGAA PflMI 1389 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1609 AACGTT AA^CGTT SapI 226 GAAGAGC GCTCTTC(1/4) SfuI 1578 TTCGAA TT^CGAA SmlI 373 CTTGAG C^T[CT][GA]AG Van91I 1389 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 325 GGTCC G^G[AT]CC 1147 GGACC BclI 62 TGATCA T^GATCA 1740 TGATCA BfaI 232 CTAG C^TAG 1678 CTAG BglII 34 AGATCT A^GATCT 1702 AGATCT BsaBI 1191 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1697 GATACAGATC Bsp1407I 1656 TGTACA T^GTACA 1691 TGTACA BspHI 1206 TCATGA T^CATGA 1239 TCATGA BsrGI 1656 TGTACA T^GTACA 1691 TGTACA Cfr13I 325 GGTCC G^G[ACGT]CC 1147 GGACC EarI 1382 CTCTTC CTCTTC(1/4) 226 GAAGAG EcoRI 621 GAATTC G^AATTC 796 GAATTC FbaI 62 TGATCA T^GATCA 1740 TGATCA Ksp632I 1382 CTCTTC CTCTTC(1/4) 226 GAAGAG MaeI 232 CTAG C^TAG 1678 CTAG MamI 1191 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1697 GATACAGATC NcoI 852 CCATGG C^CATGG 1561 CCATGG RcaI 1206 TCATGA T^CATGA 1239 TCATGA Sau96I 325 GGTCC G^G[ACGT]CC 1147 GGACC SfaNI 5 GATGC GCATC(5/9) 1191 GATGC SspBI 1656 TGTACA T^GTACA 1691 TGTACA StuI 377 AGGCCT AGG^CCT 1749 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I BbvI BsaI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI