(Circular) (Six-base) MAPSORT of: pFN21AE2041.seq Check: 3867 from: 1 to: 6664 pFN21AE2041 6664 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2670 4007 1937 Size: 733 1337 4594 Fragments arranged by size: 4594 1337 733 Acc65I G'GTAC_C Cuts at: 2082 3985 2082 Size: 1903 4761 AclI AA'CG_TT Cuts at: 1563 3615 4791 5164 1563 Size: 2052 1176 373 3063 Fragments arranged by size: 3063 2052 1176 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2126 2474 4727 6111 6379 1632 Size: 494 348 2253 1384 268 1917 Fragments arranged by size: 2253 1917 1384 494 348 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 3624 5563 6540 1203 Size: 2421 1939 977 1327 Fragments arranged by size: 2421 1939 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6664 AlwNI CAG_nnn'CTG Cuts at: 4407 5979 4407 Size: 1572 5092 ApaLI G'TGCA_C Cuts at: 4721 5877 4721 Size: 1156 5508 ApoI r'AATT_y Cuts at: 1493 2467 2626 2748 2801 2887 3773 3973 4199 Size: 974 159 122 53 86 886 200 226 Cuts at: 4199 4351 6460 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 974 886 226 200 159 152 122 86 53 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 3989 1870 Size: 73 2046 4545 Fragments arranged by size: 4545 2046 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2074 2107 2817 2850 2074 Size: 33 710 33 5888 Fragments arranged by size: 5888 710 33 33 BamHI G'GATC_C Cuts at: 3994 4361 3994 Size: 367 6297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2082 3985 5440 618 Size: 325 217 627 295 1903 1455 1842 Fragments arranged by size: 1903 1842 1455 627 325 295 217 BanII G_rGCy'C Cuts at: 1391 3983 1391 Size: 2592 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3936 928 Size: 971 2037 3656 Fragments arranged by size: 3656 2037 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4855 4889 1903 Size: 34 2918 34 3678 Fragments arranged by size: 3678 2918 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3397 4556 5766 3397 Size: 1159 1210 4295 Fragments arranged by size: 4295 1210 1159 BclI T'GATC_A Cuts at: 1548 2067 3745 1548 Size: 519 1678 4467 Fragments arranged by size: 4467 1678 519 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5275 136 Size: 107 122 71 631 605 3603 1525 Fragments arranged by size: 3603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2039 3707 6659 2039 Size: 1668 2952 2044 Fragments arranged by size: 2952 2044 1668 BlpI GC'TnA_GC Cuts at: 4081 4081 Size: 6664 Bme1580I G_kGCm'C Cuts at: 1790 4725 5881 1790 Size: 2935 1156 2573 Fragments arranged by size: 2935 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4395 5353 442 Size: 416 53 83 399 24 2978 958 1753 Fragments arranged by size: 2978 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5324 1239 Size: 4085 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2399 4789 5654 5952 2399 Size: 2390 865 298 3111 Fragments arranged by size: 3111 2390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2573 2867 2949 3213 5327 882 Size: 617 1074 294 82 264 2114 2219 Fragments arranged by size: 2219 2114 1074 617 294 264 82 BsaAI yAC'GTr Cuts at: 493 1204 3126 4455 493 Size: 711 1922 1329 2702 Fragments arranged by size: 2702 1922 1329 711 BsaBI GATnn'nnATC Cuts at: 3200 3706 6658 3200 Size: 506 2952 3206 Fragments arranged by size: 3206 2952 506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5097 5495 5769 5916 6327 5097 Size: 398 274 147 411 5434 Fragments arranged by size: 5434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6439 Fragments arranged by size: 6439 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3147 3321 3330 1186 Size: 1961 174 9 4520 Fragments arranged by size: 4520 1961 174 9 BseYI C'CCAG_C Cuts at: 5867 6363 5867 Size: 496 6168 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2669 1163 Size: 573 933 5158 Fragments arranged by size: 5158 933 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4725 4810 5881 4725 Size: 85 1071 5508 Fragments arranged by size: 5508 1071 85 BsmI GAATG_Cn' Cuts at: 4175 4268 4175 Size: 93 6571 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3983 4725 4810 5881 1391 Size: 399 2193 742 85 1071 2174 Fragments arranged by size: 2193 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 3211 3244 4553 6283 3211 Size: 33 1309 1730 3592 Fragments arranged by size: 3592 1730 1309 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2691 4019 844 Size: 1847 1328 3489 Fragments arranged by size: 3489 1847 1328 BsrBI CCG'CTC Cuts at: 1394 1823 4551 1394 Size: 429 2728 3507 Fragments arranged by size: 3507 2728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5159 5333 65 Size: 1183 3911 174 1396 Fragments arranged by size: 3911 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5308 5495 1700 Size: 110 3498 187 2869 Fragments arranged by size: 3498 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3661 3696 96 Size: 3565 35 3064 Fragments arranged by size: 3565 3064 35 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 3471 4724 5736 3471 Size: 1253 1012 4399 Fragments arranged by size: 4399 1253 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6664 BstBI TT'CG_AA Cuts at: 3584 5468 3584 Size: 1884 4780 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstXI CCAn_nnnn'nTGG Cuts at: 3856 3856 Size: 6664 BstYI r'GATC_y Cuts at: 1972 2039 2637 3029 3572 3707 3994 4361 4746 Size: 67 598 392 543 135 287 367 385 Cuts at: 4746 4763 6215 6301 6313 6659 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 598 543 392 385 367 346 287 135 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2857 3566 3765 513 Size: 552 939 853 709 199 3412 Fragments arranged by size: 3412 939 853 709 552 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5925 Fragments arranged by size: 5925 683 56 BtsI GCAGTG_nn' Cuts at: 4180 4974 4994 4180 Size: 794 20 5850 Fragments arranged by size: 5850 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6629 DraI TTT'AAA Cuts at: 3900 3968 3900 Size: 68 6596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4111 5000 6629 Size: 54 1425 50 275 18 2281 889 1629 Cuts at: 6629 8 Size: 43 Fragments arranged by size: 2281 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 3393 4594 1353 Size: 873 1167 1201 3423 Fragments arranged by size: 3423 1201 1167 873 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5251 5625 5771 387 Size: 1064 138 3662 374 146 1280 Fragments arranged by size: 3662 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2474 4727 5324 6111 6379 1239 Size: 393 494 348 2253 597 787 268 1524 Fragments arranged by size: 2253 1524 787 597 494 393 348 268 EcoNI CCTnn'n_nnAGG Cuts at: 2578 2578 Size: 6664 EcoRI G'AATT_C Cuts at: 2626 2801 3973 4351 2626 Size: 175 1172 378 4939 Fragments arranged by size: 4939 1172 378 175 EcoRV GAT'ATC Cuts at: 2868 2868 Size: 6664 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 3423 5811 1678 Size: 1745 2388 2531 Fragments arranged by size: 2531 2388 1745 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2954 2986 3300 3332 5305 Size: 32 135 32 1382 32 314 32 1973 Cuts at: 5305 5337 5379 5411 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1973 1382 314 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2836 4008 4254 669 Size: 988 281 898 1172 246 3079 Fragments arranged by size: 3079 1172 988 898 281 246 HindIII A'AGCT_T Cuts at: 748 3842 748 Size: 3094 3570 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KpnI G_GTAC'C Cuts at: 2086 3989 2086 Size: 1903 4761 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 3505 5778 5962 1305 Size: 879 1321 2273 184 2007 Fragments arranged by size: 2273 2007 1321 879 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4842 Fragments arranged by size: 4842 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3810 3822 3924 4622 4981 5140 518 Size: 648 2644 12 102 698 359 159 2042 Fragments arranged by size: 2644 2042 698 648 359 159 102 12 MspA1I CmG'CkG Cuts at: 1606 4080 4757 5905 6150 1606 Size: 2474 677 1148 245 2120 Fragments arranged by size: 2474 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2857 3566 513 Size: 552 939 853 709 3611 Fragments arranged by size: 3611 939 853 709 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 3628 4022 5567 1132 Size: 97 2399 394 1545 2229 Fragments arranged by size: 2399 2229 1545 394 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6664 PciI A'CATG_T Cuts at: 3624 5563 3624 Size: 1939 4725 PflMI CCAn_nnn'nTGG Cuts at: 1108 3400 1108 Size: 2292 4372 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4733 4765 6246 6278 4733 Size: 32 1481 32 5119 Fragments arranged by size: 5119 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2385 2417 2385 Size: 32 6632 PstI C_TGCA'G Cuts at: 830 4016 830 Size: 3186 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SapI GCTCTTCn'nnn_ Cuts at: 2226 2226 Size: 6664 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 4912 6624 4912 Size: 1712 4952 SfcI C'TryA_G Cuts at: 826 1045 4012 5147 5828 6019 826 Size: 219 2967 1135 681 191 1471 Fragments arranged by size: 2967 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 3991 1872 Size: 2119 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2378 4768 5669 5931 820 Size: 197 926 435 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 435 262 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 2384 3756 1517 Size: 867 1372 4425 Fragments arranged by size: 4425 1372 867 StyI C'CwwG_G Cuts at: 513 1065 2004 2857 3566 3958 4103 513 Size: 552 939 853 709 392 145 3074 Fragments arranged by size: 3074 939 853 709 552 392 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4691 4708 4861 5046 5491 325 Size: 1255 3111 17 153 185 445 1498 Fragments arranged by size: 3111 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3661 3696 4910 Size: 274 80 33 51 1443 1684 35 1214 Cuts at: 4910 6622 96 Size: 1712 138 Fragments arranged by size: 1712 1684 1443 1214 274 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6624 6656 6624 Size: 32 6632 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 3989 1870 Size: 2119 4545 XmnI GAAnn'nnTTC Cuts at: 2626 3511 3774 4793 2626 Size: 885 263 1019 4497 Fragments arranged by size: 4497 1019 885 263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI