Restriction map for
pFN21AE5230
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 472 GTCGAC GT^[AC][GT]AC
AciI 165 CCGC CCGC(-3/-1)
AflIII 530 ACGTGT A^C[GA][CT]GT
BanII 259 GGGCTC G[GA]GC[CT]^C
BbvCI 159 CCTCAGC CCTCAGC(-5/-2)
BbvI 325 GCAGC GCAGC(8/12)
BcgI 173 CGAGTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 136 GCTGAGC GC^T[ACGT]AGC
BmyI 259 GGGCTC G[AGT]GC[ACT]^C
BpmI 142 CTGGAG CTGGAG(16/14)
Bpu1102I 136 GCTGAGC GC^T[ACGT]AGC
BseRI 145 GAGGAG GAGGAG(10/8)
BsmAI 22 GAGAC GTCTC(1/5)
Bsp1286I 259 GGGCTC G[AGT]GC[ACT]^C
BssKI 407 CCTGG ^CC[ACGT]GG
BssSI 522 CACGAG CACGAG(-5/-1)
BstEII 347 GGTCACC G^GT[ACGT]ACC
BstNI 407 CCTGG CC^[AT]GG
BstPI 347 GGTCACC G^GT[ACGT]ACC
BstYI 447 AGATCC [GA]^GATC[CT]
Cac8I 132 GCGAGC GC[ACGT]^[ACGT]GC
CelII 136 GCTGAGC GC^T[ACGT]AGC
DraII 149 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 478 CTCTTC CTCTTC(1/4)
EcoO109I 149 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 347 GGTCACC G^GT[ACGT]ACC
EcoRII 407 CCTGG ^CC[AT]GG
EcoT22I 224 ATGCAT ATGCA^T
HaeIII 104 GGCC GG^CC
HincII 472 GTCGAC GT[CT]^[GA]AC
HindII 472 GTCGAC GT[CT]^[GA]AC
HinfI 174 GAGTC G^A[ACGT]TC
Ksp632I 478 CTCTTC CTCTTC(1/4)
MflI 447 AGATCC [GA]^GATC[CT]
MseI 599 TTAA T^TAA
MvaI 407 CCTGG CC^[AT]GG
NlaIV 150 GGACCC GG[ACGT]^[ACGT]CC
NsiI 224 ATGCAT ATGCA^T
PleI 174 GAGTC GAGTC(4/5)
PpuMI 149 AGGACCC [GA]G^G[AT]CC[CT]
PstI 323 CTGCAG CTGCA^G
SalI 472 GTCGAC G^TCGAC
ScrFI 407 CCTGG CC^[ACGT]GG
SexAI 406 ACCTGGT A^CC[AT]GGT
SfcI 323 CTGCAG C^T[GA][CT]AG
TaqI 473 TCGA T^CGA
Tru9I 599 TTAA T^TAA
TseI 325 GCAGC G^C[AT]GC
TthHB8I 473 TCGA T^CGA
XhoII 447 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 156 GATCC GGATC(4/5)
448 GATCC
AvaI 87 CCCGAG C^[CT]CG[GA]G
278 CTCGGG
AvaII 150 GGACC G^G[AT]CC
426 GGTCC
BsoBI 87 CCCGAG C^[CT]CG[GA]G
278 CTCGGG
Cfr13I 150 GGACC G^G[ACGT]CC
426 GGTCC
DpnII 156 GATC ^GATC
448 GATC
EcoT14I 100 CCAAGG C^C[AT][AT]GG
267 CCTTGG
Fnu4HI 164 GCCGC GC^[ACGT]GC
325 GCAGC
HphI 552 GGTGA GGTGA(8/7)
349 TCACC
ItaI 164 GCCGC GC^[ACGT]GC
325 GCAGC
MaeII 241 ACGT A^CGT
530 ACGT
MboI 156 GATC ^GATC
448 GATC
MwoI 132 GCGAGCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
319 GCTTCTGCAGC
NdeII 156 GATC ^GATC
448 GATC
Sau3AI 156 GATC ^GATC
448 GATC
Sau96I 150 GGACC G^G[ACGT]CC
426 GGTCC
StyI 100 CCAAGG C^C[AT][AT]GG
267 CCTTGG
Tsp45I 348 GTCAC ^GT[GC]AC
527 GTGAC
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AluI BsaJI BsrI BstF5I DdeI
FokI MaeIII MboII MnlI NlaIII
SfaNI Tsp509I TspRI