(Circular) (Six-base) MAPSORT of: pFN21AE5230.seq Check: 9974 from: 1 to: 5329 pFN21AE5230 5329 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3139 278 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2478 2672 1937 Size: 541 194 4594 Fragments arranged by size: 4594 541 194 Acc65I G'GTAC_C Cuts at: 2650 2650 Size: 5329 AclI AA'CG_TT Cuts at: 1563 3456 3829 1563 Size: 1893 373 3063 Fragments arranged by size: 3063 1893 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 2325 2470 2564 3392 4776 5044 1632 Size: 612 81 145 94 828 1384 268 1917 Fragments arranged by size: 1917 1384 828 612 268 145 94 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5132 AflIII A'CryG_T Cuts at: 1203 2535 4228 5205 1203 Size: 1332 1693 977 1327 Fragments arranged by size: 1693 1332 1327 977 AgeI A'CCGG_T Cuts at: 4160 4160 Size: 5329 AhdI GACnn_n'nnGTC Cuts at: 4058 4058 Size: 5329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5329 AlwNI CAG_nnn'CTG Cuts at: 3072 4644 3072 Size: 1572 3757 ApaLI G'TGCA_C Cuts at: 3386 4542 3386 Size: 1156 4173 ApoI r'AATT_y Cuts at: 1493 2638 2864 3016 5125 1493 Size: 1145 226 152 2109 1697 Fragments arranged by size: 2109 1697 1145 226 152 AseI AT'TA_AT Cuts at: 160 1032 3883 160 Size: 872 2851 1606 Fragments arranged by size: 2851 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2283 2654 1870 Size: 73 149 191 371 4545 Fragments arranged by size: 4545 371 191 149 73 BamHI G'GATC_C Cuts at: 2659 3026 2659 Size: 367 4962 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2650 4105 618 Size: 325 217 627 863 1455 1842 Fragments arranged by size: 1842 1455 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2268 2648 1391 Size: 877 380 4072 Fragments arranged by size: 4072 877 380 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4358 BbvCI CC'TCA_GC Cuts at: 2165 2165 Size: 5329 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2167 2201 3520 3554 1903 Size: 34 230 34 1319 34 3678 Fragments arranged by size: 3678 1319 230 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3221 4431 3221 Size: 1210 4119 BclI T'GATC_A Cuts at: 1548 1548 Size: 5329 BfrBI ATG'CAT Cuts at: 2231 2231 Size: 5329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3940 136 Size: 107 122 71 631 605 2268 1525 Fragments arranged by size: 2268 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5324 5324 Size: 5329 BlpI GC'TnA_GC Cuts at: 2142 2746 2142 Size: 604 4725 Bme1580I G_kGCm'C Cuts at: 1790 3390 4546 1790 Size: 1600 1156 2573 Fragments arranged by size: 2573 1600 1156 BmgBI CAC'GTC Cuts at: 1318 2536 1318 Size: 1218 4111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3060 4018 442 Size: 416 53 83 399 24 1643 958 1753 Fragments arranged by size: 1753 1643 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5329 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2050 2050 Size: 5329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 3989 1239 Size: 929 1821 2579 Fragments arranged by size: 2579 1821 929 Bpu10I CC'TnA_GC Cuts at: 2165 2165 Size: 5329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3454 4319 4617 3454 Size: 865 298 4166 Fragments arranged by size: 4166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3992 882 Size: 617 2493 2219 Fragments arranged by size: 2493 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3120 493 Size: 711 1916 2702 Fragments arranged by size: 2702 1916 711 BsaBI GATnn'nnATC Cuts at: 5323 5323 Size: 5329 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3136 3518 4153 275 Size: 53 83 186 936 1603 382 635 1451 Fragments arranged by size: 1603 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3762 4160 4434 4581 4992 3762 Size: 398 274 147 411 4099 Fragments arranged by size: 4099 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5104 Fragments arranged by size: 5104 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 1186 Size: 979 4350 BseYI C'CCAG_C Cuts at: 2563 4532 5028 2563 Size: 1969 496 2864 Fragments arranged by size: 2864 1969 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4756 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2779 3540 4568 1540 Size: 275 186 778 761 1028 2301 Fragments arranged by size: 2301 1028 778 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3390 3475 4546 3390 Size: 85 1071 4173 Fragments arranged by size: 4173 1071 85 BsmI GAATG_Cn' Cuts at: 2840 2933 2840 Size: 93 5236 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2648 3390 3475 4546 1391 Size: 399 478 380 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 380 85 BspEI T'CCGG_A Cuts at: 4992 4992 Size: 5329 BspHI T'CATG_A Cuts at: 3218 4948 3218 Size: 1730 3599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2684 844 Size: 1840 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3216 1394 Size: 429 1393 3507 Fragments arranged by size: 3507 1393 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3824 3998 65 Size: 1183 2576 174 1396 Fragments arranged by size: 2576 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3973 4160 1700 Size: 110 2163 187 2869 Fragments arranged by size: 2869 2163 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5329 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5329 BssSI C'ACGA_G Cuts at: 2527 3389 4401 2527 Size: 862 1012 3455 Fragments arranged by size: 3455 1012 862 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5329 BstBI TT'CG_AA Cuts at: 4133 4133 Size: 5329 BstEII G'GTnAC_C Cuts at: 1184 2352 1184 Size: 1168 4161 BstYI r'GATC_y Cuts at: 1972 2452 2659 3026 3411 3428 4880 4966 4978 Size: 480 207 367 385 17 1452 86 12 Cuts at: 4978 5324 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 480 385 367 346 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4777 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4590 Fragments arranged by size: 4590 683 56 BtsI GCAGTG_nn' Cuts at: 2433 2845 3639 3659 2433 Size: 412 794 20 4103 Fragments arranged by size: 4103 794 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5294 DraI TTT'AAA Cuts at: 2633 2633 Size: 5329 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4336 809 Size: 507 3020 1802 Fragments arranged by size: 3020 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2776 3665 5294 Size: 54 1425 50 275 18 946 889 1629 Cuts at: 5294 8 Size: 43 Fragments arranged by size: 1629 1425 946 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2776 1537 Size: 275 964 4090 Fragments arranged by size: 4090 964 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 3259 1353 Size: 1136 770 3423 Fragments arranged by size: 3423 1136 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3916 4290 4436 387 Size: 1064 138 2327 374 146 1280 Fragments arranged by size: 2327 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2244 2325 2470 2564 3392 3989 Size: 393 536 76 81 145 94 828 597 Cuts at: 3989 4776 5044 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 597 536 393 268 145 94 81 76 EcoO109I rG'GnC_Cy Cuts at: 2155 2155 Size: 5329 EcoRI G'AATT_C Cuts at: 2638 3016 2638 Size: 378 4951 FspI TGC'GCA Cuts at: 3835 3835 Size: 5329 HaeII r_GCGC'y Cuts at: 1678 4476 1678 Size: 2798 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3970 4002 4044 4076 1373 Size: 32 135 32 2398 32 42 32 2626 Fragments arranged by size: 2626 2398 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 2673 2919 669 Size: 988 281 541 194 246 3079 Fragments arranged by size: 3079 988 541 281 246 194 HindIII A'AGCT_T Cuts at: 748 748 Size: 5329 HpaI GTT'AAC Cuts at: 2919 2919 Size: 5329 KpnI G_GTAC'C Cuts at: 2654 2654 Size: 5329 MfeI C'AATT_G Cuts at: 2928 2928 Size: 5329 MluI A'CGCG_T Cuts at: 5205 5205 Size: 5329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4443 4627 1305 Size: 3138 184 2007 Fragments arranged by size: 3138 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3507 Fragments arranged by size: 3507 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3287 3646 3805 518 Size: 648 2121 359 159 2042 Fragments arranged by size: 2121 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2745 3422 4570 4815 1606 Size: 1139 677 1148 245 2120 Fragments arranged by size: 2120 1148 1139 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4777 NdeI CA'TA_TG Cuts at: 387 387 Size: 5329 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5329 NotI GC'GGCC_GC Cuts at: 2776 2776 Size: 5329 NsiI A_TGCA'T Cuts at: 2233 2233 Size: 5329 NspI r_CATG'y Cuts at: 1132 1229 2687 4232 1132 Size: 97 1458 1545 2229 Fragments arranged by size: 2229 1545 1458 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5329 PciI A'CATG_T Cuts at: 4228 4228 Size: 5329 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5329 PmeI GTTT'AAAC Cuts at: 2633 2633 Size: 5329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 3430 4911 4943 3398 Size: 32 1481 32 3784 Fragments arranged by size: 3784 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2155 Size: 5329 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5329 PsiI TTA'TAA Cuts at: 2899 2899 Size: 5329 PstI C_TGCA'G Cuts at: 830 2332 2681 830 Size: 1502 349 3478 Fragments arranged by size: 3478 1502 349 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5329 SalI G'TCGA_C Cuts at: 1936 2477 2671 1936 Size: 541 194 4594 Fragments arranged by size: 4594 541 194 SbfI CC_TGCA'GG Cuts at: 2681 2681 Size: 5329 ScaI AGT'ACT Cuts at: 3577 5289 3577 Size: 1712 3617 SexAI A'CCwGG_T Cuts at: 2411 2411 Size: 5329 SfcI C'TryA_G Cuts at: 826 1045 2328 2677 3812 4493 4684 826 Size: 219 1283 349 1135 681 191 1471 Fragments arranged by size: 1471 1283 1135 681 349 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5329 SmaI CCC'GGG Cuts at: 1872 2656 1872 Size: 784 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3433 4334 4596 820 Size: 197 926 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3120 493 Size: 2627 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5329 SphI G_CATG'C Cuts at: 1132 2687 1132 Size: 1555 3774 SspI AAT'ATT Cuts at: 5 52 3253 5 Size: 47 3201 2081 Fragments arranged by size: 3201 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5329 StyI C'CwwG_G Cuts at: 513 1065 2105 2272 2768 513 Size: 552 1040 167 496 3074 Fragments arranged by size: 3074 1040 552 496 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2613 3356 3373 3526 3711 4156 325 Size: 1255 1033 743 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1033 743 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3575 5287 96 Size: 274 80 33 51 1443 1598 1712 138 Fragments arranged by size: 1712 1598 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2164 2196 2559 2591 5289 5321 2164 Size: 32 363 32 2698 32 2172 Fragments arranged by size: 2698 2172 363 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5329 XbaI T'CTAG_A Cuts at: 2665 2665 Size: 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5329 XmaI C'CCGG_G Cuts at: 1870 2654 1870 Size: 784 4545 XmnI GAAnn'nnTTC Cuts at: 3458 3458 Size: 5329 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3137 276 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI