(Linear) (Six-base) MAPSORT of: KIEE5230.seq  Check: 6914  from: 1  to: 624



KIEE5230.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:51  ..

AccI GT'mk_AC

Cuts at:      0     473     624 
   Size:        473     151
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     320     465     559     624 
   Size:        239      81     145      94      65

  Fragments arranged by size: 

                 239     145      94      81      65
 
AflIII A'CryG_T

Cuts at:      0     530     624 
   Size:        530      94
 
AvaI C'yCGr_G

Cuts at:      0      87     278     624 
   Size:         87     191     346

  Fragments arranged by size: 

                 346     191      87
 
BanII G_rGCy'C

Cuts at:      0     263     624 
   Size:        263     361
 
BbvCI CC'TCA_GC

Cuts at:      0     160     624 
   Size:        160     464
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     162     196     624 
   Size:        162      34     428

  Fragments arranged by size: 

                 428     162      34
 
BfrBI ATG'CAT

Cuts at:      0     226     624 
   Size:        226     398
 
BlpI GC'TnA_GC

Cuts at:      0     137     624 
   Size:        137     487
 
BmgBI CAC'GTC

Cuts at:      0     531     624 
   Size:        531      93
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      45     624 
   Size:         45     579
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     624 
   Size:        163     461
 
Bpu10I CC'TnA_GC

Cuts at:      0     160     624 
   Size:        160     464
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     624 
   Size:        160     464
 
BseYI C'CCAG_C

Cuts at:      0     558     624 
   Size:        558      66
 
Bsp1286I G_dGCh'C

Cuts at:      0     263     624 
   Size:        263     361
 
BssSI C'ACGA_G

Cuts at:      0     522     624 
   Size:        522     102
 
BstEII G'GTnAC_C

Cuts at:      0     347     624 
   Size:        347     277
 
BstYI r'GATC_y

Cuts at:      0     447     624 
   Size:        447     177
 
BtsI GCAGTG_nn'

Cuts at:      0     428     624 
   Size:        428     196
 
EarI CTCTTCn'nnn_

Cuts at:      0     484     624 
   Size:        484     140
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     239     320     465     559     624 
   Size:        163      76      81     145      94      65

  Fragments arranged by size: 

                 163     145      94      81      76      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     624 
   Size:        150     474
 
HincII GTy'rAC

Cuts at:      0     474     624 
   Size:        474     150
 
NsiI A_TGCA'T

Cuts at:      0     228     624 
   Size:        228     396
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     624 
   Size:        150     474
 
PstI C_TGCA'G

Cuts at:      0     327     624 
   Size:        327     297
 
SalI G'TCGA_C

Cuts at:      0     472     624 
   Size:        472     152
 
SexAI A'CCwGG_T

Cuts at:      0     406     624 
   Size:        406     218
 
SfcI C'TryA_G

Cuts at:      0     323     624 
   Size:        323     301
 
StyI C'CwwG_G

Cuts at:      0     100     267     624 
   Size:        100     167     357

  Fragments arranged by size: 

                 357     167     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     608     624 
   Size:        608      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     159     191     554     586     624 
   Size:        159      32     363      32      38

  Fragments arranged by size: 

                 363     159      38      32      32
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AvaI    BanII    BbvCI     BcgI    BfrBI
     BlpI    BmgBI     BplI     BpmI   Bpu10I    BseRI    BseYI Bsp1286I
    BssSI   BstEII    BstYI     BtsI     EarI  Eco57MI EcoO109I   HincII
     NsiI    PpuMI     PstI     SalI    SexAI     SfcI     StyI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BciVI     BclI     BglI    BglII Bme1580I     BmrI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI