##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HpaI        KasI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        448  GGTACC               G^GTACC
AocI          795  CCTGAGG              CC^T[ACGT]AGG
Asp718I       448  GGTACC               G^GTACC
BanI          448  GGTACC               G^G[CT][GA]CC
BsaAI         563  CACGTA               [CT]AC^GT[GA]
BsgI          639  GTGCAG               GTGCAG(16/14)
BsmI          835  GAATGC               GAATGC(1/-1)
BsrDI         803  GCAATG               GCAATG(2/0)
BstAPI        408  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         672  CCTGG                CC^[AT]GG
Bsu36I        795  CCTGAGG              CC^T[ACGT]AGG
CfoI            7  GCGC                 GCG^C
DraII        1378  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          476  CCGTGG               C^C[GA][CT]GG
Eco81I        795  CCTGAGG              CC^T[ACGT]AGG
EcoNI          63  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1378  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        672  CCTGG                ^CC[AT]GG
FauI         1131  GCGGG                CCCGC(4/6)
HgaI          664  GACGC                GACGC(5/10)
HhaI            7  GCGC                 GCG^C
HinP1I          7  GCGC                 G^CGC
HindIII       401  AAGCTT               A^AGCTT
HphI          792  TCACC                GGTGA(8/7)
KpnI          448  GGTACC               GGTAC^C
MvaI          672  CCTGG                CC^[AT]GG
NspI          611  GCATGT               [GA]CATG^[CT]
PpuMI        1378  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          889  CTGCAG               CTGCA^G
SfaNI         414  GATGC                GCATC(5/9)


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwI          420  GGATC                GGATC(4/5)
              501  GGATC               
BpmI         1046  CTCCAG               CTGGAG(16/14)
              673  CTGGAG              
MaeIII        910  GTGAC                ^GT[ACGT]AC
             1202  GTCAC               
MspA1I        774  CAGCTG               C[AC]G^C[GT]G
              886  CAGCTG              
PvuII         774  CAGCTG               CAG^CTG
              886  CAGCTG              
TaqI          105  TCGA                 T^CGA
              504  TCGA                
TfiI          759  GATTC                G^A[AT]TC
             1180  GAATC               
Tsp45I        910  GTGAC                ^GT[GC]AC
             1202  GTCAC               
TthHB8I       105  TCGA                 T^CGA
              504  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaI        AvaII       BbvI        BcnI        BfaI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       BsrI        BssKI       BstF5I      
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRI       Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        Ksp632I     MaeI        
MaeII       MboI        MboII       MnlI        MseI        
MspI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        Tru9I       TseI        Tsp509I     
TspRI