Restriction map for
pFN21AE4051
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI MamI
MfeI MluI MroI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 39 CGCG CG^CG
AlwI 855 GGATC GGATC(4/5)
ApaI 760 GGGCCC GGGCC^C
AspHI 275 GAGCTC G[AT]GC[AT]^C
BalI 617 TGGCCA TGG^CCA
BanI 714 GGTGCC G^G[CT][GA]CC
BbrPI 436 CACGTG CAC^GTG
BfaI 425 CTAG C^TAG
BsaAI 436 CACGTG [CT]AC^GT[GA]
BsaI 110 GGTCTC GGTCTC(1/5)
BsiHKAI 275 GAGCTC G[AT]GC[AT]^C
BsmFI 50 GGGAC GGGAC(10/14)
BsmI 684 GAATGC GAATGC(1/-1)
BstUI 39 CGCG CG^CG
BstYI 855 GGATCT [GA]^GATC[CT]
EaeI 617 TGGCCA [CT]^GGCC[GA]
EcoNI 846 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 794 GAATTC G^AATTC
EcoT22I 118 ATGCAT ATGCA^T
HincII 448 GTCAAC GT[CT]^[GA]AC
HindII 448 GTCAAC GT[CT]^[GA]AC
HindIII 553 AAGCTT A^AGCTT
HphI 282 GGTGA GGTGA(8/7)
KspI 38 CCGCGG CCGC^GG
MaeI 425 CTAG C^TAG
MaeIII 292 GTGAC ^GT[ACGT]AC
MflI 855 GGATCT [GA]^GATC[CT]
MluNI 617 TGGCCA TGG^CCA
MscI 617 TGGCCA TGG^CCA
MvnI 39 CGCG CG^CG
NsiI 118 ATGCAT ATGCA^T
PleI 562 GAGTC GAGTC(4/5)
PmaCI 436 CACGTG CAC^GTG
PmlI 436 CACGTG CAC^GTG
PstI 364 CTGCAG CTGCA^G
SacI 275 GAGCTC GAGCT^C
SacII 38 CCGCGG CCGC^GG
SfcI 364 CTGCAG C^T[GA][CT]AG
Tsp45I 292 GTGAC ^GT[GC]AC
XcmI 629 CCAGTGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 855 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 354 GAATTT [GA]^AATT[CT]
794 GAATTC
ApoI 354 GAATTT [GA]^AATT[CT]
794 GAATTC
BglI 36 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
494 GCCACCTGGGC
BpmI 719 CTCCAG CTGGAG(16/14)
559 CTGGAG
BsmAI 111 GTCTC GTCTC(1/5)
150 GTCTC
BsrBI 569 GAGCGG CCGCTC(-3/-3)
665 GAGCGG
BsrI 302 ACTGG ACTGG(1/-1)
629 CCAGT
DraII 759 AGGGCCC [GA]G^G[ACGT]CC[CT]
760 GGGCCCC
DsaI 38 CCGCGG C^C[GA][CT]GG
135 CCACGG
EarI 742 CTCTTC CTCTTC(1/4)
634 GAAGAG
EcoO109I 759 AGGGCCC [GA]G^G[ACGT]CC[CT]
760 GGGCCCC
FauI 37 CCCGC CCCGC(4/6)
40 GCGGG
Ksp632I 742 CTCTTC CTCTTC(1/4)
634 GAAGAG
MseI 265 TTAA T^TAA
320 TTAA
PvuII 145 CAGCTG CAG^CTG
339 CAGCTG
SapI 634 GAAGAGC GCTCTTC(1/4)
741 GCTCTTC
TfiI 598 GATTC G^A[AT]TC
812 GAATC
Tru9I 265 TTAA T^TAA
320 TTAA
Tsp509I 355 AATT ^AATT
795 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI MaeII MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TseI TspRI