Restriction map for
pFN21AE4051
(Circular) (Six-base) MAPSORT of: pFN21AE4051.seq Check: 9491 from: 1 to: 5587
pFN21AE4051 5587 bp
With 182 enzymes: SgfI *
March 28, 2012 13:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2715 3650 5034 5302 1632
Size: 1083 935 1384 268 1917
Fragments arranged by size:
1917 1384 1083 935 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 4486 5463 1203
Size: 3283 977 1327
Fragments arranged by size:
3283 1327 977
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2148 1184
Size: 964 4623
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2370 2402 2370
Size: 32 5555
AlwNI CAG_nnn'CTG
Cuts at: 3330 4902 3330
Size: 1572 4015
ApaI G_GGCC'C
Cuts at: 2769 2769
Size: 5587
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2359 2799 2896 3122 3274 5383 1493
Size: 866 440 97 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 440 226 152 97
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2912 1870
Size: 73 969 4545
Fragments arranged by size:
4545 969 73
BamHI G'GATC_C
Cuts at: 2917 3284 2917
Size: 367 5220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2719 2908 4363 618
Size: 325 217 627 932 189 1455 1842
Fragments arranged by size:
1842 1455 932 627 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2284 2735 2769 2906 1391
Size: 893 451 34 137 4072
Fragments arranged by size:
4072 893 451 137 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3479 4689 3479
Size: 1210 4377
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BfrBI ATG'CAT
Cuts at: 2125 2125
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2047 2505 4198
Size: 107 122 71 631 605 375 458 1693
Cuts at: 4198 136
Size: 1525
Fragments arranged by size:
1693 1525 631 605 458 375 122 107
71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 2510 2769 3648 4804 1790
Size: 720 259 879 1156 2573
Fragments arranged by size:
2573 1156 879 720 259
BmgBI CAC'GTC
Cuts at: 1318 2581 1318
Size: 1263 4324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3318 4276 442
Size: 416 53 83 399 24 1901 958 1753
Fragments arranged by size:
1901 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2264 2264
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 2709 4247 1239
Size: 1346 124 1538 2579
Fragments arranged by size:
2579 1538 1346 124
Bpu10I CC'TnA_GC
Cuts at: 2283 2283
Size: 5587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2121 4250 882
Size: 617 622 2129 2219
Fragments arranged by size:
2219 2129 622 617
BsaAI yAC'GTr
Cuts at: 493 1204 2443 3378 493
Size: 711 1239 935 2702
Fragments arranged by size:
2702 1239 935 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4020 4418 4692 4839 5250 4020
Size: 398 274 147 411 4357
Fragments arranged by size:
4357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2271 2301 2370 2400 2565
Size: 30 165 30 868 30 69 30 165
Cuts at: 2565 2595 2835 2859 2865 2889 1178
Size: 30 240 24 6 24 3876
Fragments arranged by size:
3876 868 240 165 165 69 30 30
30 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5587
BseYI C'CCAG_C
Cuts at: 2346 2742 4790 5286 2346
Size: 396 2048 496 2647
Fragments arranged by size:
2647 2048 496 396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5014
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2284 3648 3733 4804 2284
Size: 1364 85 1071 3067
Fragments arranged by size:
3067 1364 1071 85
BsmI GAATG_Cn'
Cuts at: 2695 3098 3191 2695
Size: 403 93 5091
Fragments arranged by size:
5091 403 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2510 2735 2769 2906 3648 3733
Size: 399 494 226 225 34 137 742 85
Cuts at: 3733 4804 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 226 225 137
85 34
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 3476 5206 3476
Size: 1730 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2942 844
Size: 2098 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2576 2672 3474 1394
Size: 429 753 96 802 3507
Fragments arranged by size:
3507 802 753 429 96
BsrDI GCAATG_nn'
Cuts at: 65 1248 4082 4256 65
Size: 1183 2834 174 1396
Fragments arranged by size:
2834 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5587
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5587
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5587
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2860 2917 3284 3669 3686 5138 5224 5236
Size: 888 57 367 385 17 1452 86 12
Cuts at: 5236 5582 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 888 385 367 346 86 57
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2140 513
Size: 552 939 39 97 3960
Fragments arranged by size:
3960 939 552 97 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2112 487
Size: 683 56 886 3962
Fragments arranged by size:
3962 886 683 56
BtsI GCAGTG_nn'
Cuts at: 2625 3103 3897 3917 2625
Size: 478 794 20 4295
Fragments arranged by size:
4295 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2502 2537 529
Size: 35 1938 35 3579
Fragments arranged by size:
3579 1938 35 35
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2622 3034 3923
Size: 54 1425 50 275 18 792 412 889
Cuts at: 3923 5552 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 792 412 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 2753 3517 1353
Size: 1281 119 764 3423
Fragments arranged by size:
3423 1281 764 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4174 4548 4694 387
Size: 1064 138 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2282 2282
Size: 5587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2585 2709 2715 3650 4247 5034 5302
Size: 393 953 124 6 935 597 787 268
Cuts at: 5302 1239
Size: 1524
Fragments arranged by size:
1524 953 935 787 597 393 268 124
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 2855
Size: 5587
EcoO109I rG'GnC_Cy
Cuts at: 2765 2766 2765
Size: 1 5586
EcoRI G'AATT_C
Cuts at: 2799 2896 3274 2799
Size: 97 378 5112
Fragments arranged by size:
5112 378 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2252 2252
Size: 5587
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 2393 2425 2535
Size: 32 135 32 518 32 271 32 110
Cuts at: 2535 2567 4228 4260 4302 4334 1373
Size: 32 1661 32 42 32 2626
Fragments arranged by size:
2626 1661 518 271 135 110 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 2931 3177 669
Size: 988 281 517 476 246 3079
Fragments arranged by size:
3079 988 517 476 281 246
HindIII A'AGCT_T
Cuts at: 748 2558 748
Size: 1810 3777
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 10
Size: 54 1425 343 792 2973
Fragments arranged by size:
2973 1425 792 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3545 3904 4063 518
Size: 648 2379 359 159 2042
Fragments arranged by size:
2379 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2152 2340 2346 3003 3680 4828 5073
Size: 439 107 188 6 657 677 1148 245
Cuts at: 5073 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 657 439 245 188 107
6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4096
Fragments arranged by size:
4096 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NsiI A_TGCA'T
Cuts at: 2127 2127
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2945 4490 1132
Size: 97 1716 1545 2229
Fragments arranged by size:
2229 1716 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 4486 4486
Size: 5587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5587
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PmlI CAC'GTG
Cuts at: 2443 2443
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2370 2402 3656 3688 5169 5201 2370
Size: 32 1254 32 1481 32 2756
Fragments arranged by size:
2756 1481 1254 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PspOMI G'GGCC_C
Cuts at: 2765 2765
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2373 2939 830
Size: 1543 566 3478
Fragments arranged by size:
3478 1543 566
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
PvuII CAG'CTG
Cuts at: 2152 2346 2152
Size: 194 5393
SacI G_AGCT'C
Cuts at: 2284 2284
Size: 5587
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SapI GCTCTTCn'nnn_
Cuts at: 2634 2753 2634
Size: 119 5468
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2369 2935 4070 4751 4942 826
Size: 219 1324 566 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 681 566 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3691 4592 4854 820
Size: 197 926 1748 901 262 1553
Fragments arranged by size:
1748 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5
Size: 47 3459 2081
Fragments arranged by size:
3459 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 2004 2051 2587 2736 3026 513
Size: 552 939 47 536 149 290 3074
Fragments arranged by size:
3074 939 552 536 290 149 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2693 3614 3631 3784 3969 4414 325
Size: 1255 1113 921 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1113 921 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3833 5545 96
Size: 274 80 33 51 1443 1856 1712 138
Fragments arranged by size:
1856 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2271 2303 2565 2597 2620 2652 5547 5579 2271
Size: 32 262 32 23 32 2895 32 2279
Fragments arranged by size:
2895 2279 262 32 32 32 32 23
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 3716 3716
Size: 5587
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI