################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccII AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI CelII Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshAI PvuI PvuII RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccIII 1352 TCCGGA T^CCGGA AclI 2734 AACGTT AA^CGTT AfaI 298 GTAC GT^AC AlwNI 1162 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 2766 GGACC G^G[AT]CC BalI 2777 TGGCCA TGG^CCA BanII 2994 GAGCTC G[GA]GC[CT]^C BbrPI 643 CACGTG CAC^GTG BbsI 397 GAAGAC GAAGAC(2/6) BcgI 2055 GCAGTTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 982 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1677 AGATCT A^GATCT BpuAI 397 GAAGAC GAAGAC(2/6) BsaBI 2362 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1352 TCCGGA [AT]^CCGG[AT] BseAI 1352 TCCGGA T^CCGGA BseRI 2752 GAGGAG GAGGAG(10/8) BsgI 144 GTGCAG GTGCAG(16/14) BsmBI 2065 CGTCTC CGTCTC(1/5) Bsp1407I 297 TGTACA T^GTACA BspDI 1225 ATCGAT AT^CGAT BspEI 1352 TCCGGA T^CCGGA BspLU11I 8 ACATGT A^CATGT BspMI 34 ACCTGC ACCTGC(4/8) BsrGI 297 TGTACA T^GTACA Bst1107I 323 GTATAC GTA^TAC BstBI 1392 TTCGAA TT^CGAA BstZ17I 323 GTATAC GTA^TAC ClaI 1225 ATCGAT AT^CGAT DraII 2765 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1430 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2519 CCATGG C^C[GA][CT]GG EaeI 2777 TGGCCA [CT]^GGCC[GA] EcoO109I 2765 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 955 GAATTC G^AATTC EcoT14I 2519 CCATGG C^C[AT][AT]GG HgaI 1361 GACGC GACGC(5/10) HincII 155 GTCGAC GT[CT]^[GA]AC HindII 155 GTCGAC GT[CT]^[GA]AC MamI 2362 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2777 TGGCCA TGG^CCA MroI 1352 TCCGGA T^CCGGA MscI 2777 TGGCCA TGG^CCA MspA1I 2980 CAGCGG C[AC]G^C[GT]G NcoI 2519 CCATGG C^CATGG NspI 8 ACATGT [GA]CATG^[CT] NspV 1392 TTCGAA TT^CGAA PaeR7I 2068 CTCGAG C^TCGAG PflMI 174 CCAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 643 CACGTG CAC^GTG PmlI 643 CACGTG CAC^GTG PpuMI 2765 GGGACCC [GA]G^G[AT]CC[CT] Psp1406I 2734 AACGTT AA^CGTT PstI 2480 CTGCAG CTGCA^G RsaI 298 GTAC GT^AC SacI 2994 GAGCTC GAGCT^C SalI 155 GTCGAC G^TCGAC SfuI 1392 TTCGAA TT^CGAA SmaI 2769 CCCGGG CCC^GGG SspBI 297 TGTACA T^GTACA StyI 2519 CCATGG C^C[AT][AT]GG Van91I 174 CCAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2068 CTCGAG C^TCGAG XmaCI 2769 CCCGGG C^CCGGG XmaI 2769 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 155 GTCGAC GT^[AC][GT]AC 323 GTATAC AocI 378 CCTAAGG CC^T[ACGT]AGG 894 CCTCAGG AspHI 1643 GTGCTC G[AT]GC[AT]^C 2994 GAGCTC AvaI 2068 CTCGAG C^[CT]CG[GA]G 2769 CCCGGG BmyI 1643 GTGCTC G[AGT]GC[ACT]^C 2994 GAGCTC BsaAI 643 CACGTG [CT]AC^GT[GA] 2084 TACGTG BsaI 1909 GGTCTC GGTCTC(1/5) 2612 GGTCTC BsiHKAI 1643 GTGCTC G[AT]GC[AT]^C 2994 GAGCTC BsmFI 2765 GGGAC GGGAC(10/14) 1824 GTCCC BsoBI 2068 CTCGAG C^[CT]CG[GA]G 2769 CCCGGG Bsp1286I 1643 GTGCTC G[AGT]GC[ACT]^C 2994 GAGCTC Bsu36I 378 CCTAAGG CC^T[ACGT]AGG 894 CCTCAGG CfoI 74 GCGC GCG^C 2703 GCGC Cfr13I 1489 GGGCC G^G[ACGT]CC 2766 GGACC EarI 2433 GAAGAG CTCTTC(1/4) 2716 GAAGAG Eco81I 378 CCTAAGG CC^T[ACGT]AGG 894 CCTCAGG FauI 1010 CCCGC CCCGC(4/6) 761 GCGGG HhaI 74 GCGC GCG^C 2703 GCGC HinP1I 74 GCGC G^CGC 2703 GCGC Ksp632I 2433 GAAGAG CTCTTC(1/4) 2716 GAAGAG Sau96I 1489 GGGCC G^G[ACGT]CC 2766 GGACC SspI 1072 AATATT AAT^ATT 1598 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AflIII AluI AlwI ApoI AseI AsnI Asp700I BbvI BcnI BfaI BpmI BsaJI BsiYI BslI BsmAI BsmI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PshBI Sau3AI ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII XmnI