Restriction map for
pFN21AE5821
(Circular) (Six-base) MAPSORT of: pFN21AE5821.seq Check: 7664 from: 1 to: 7828
pFN21AE5821 7828 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5638 278
Size: 53 83 186 936 4102 2468
Fragments arranged by size:
4102 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2161 2329 5171 1937
Size: 224 168 2842 4594
Fragments arranged by size:
4594 2842 224 168
Acc65I G'GTAC_C
Cuts at: 5149 5149
Size: 7828
AclI AA'CG_TT
Cuts at: 1563 4740 5955 6328 1563
Size: 3177 1215 373 3063
Fragments arranged by size:
3177 3063 1215 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2006 2963 3977 4224 4238 5891 7275 7543
Size: 374 957 1014 247 14 1653 1384 268
Cuts at: 7543 1632
Size: 1917
Fragments arranged by size:
1917 1653 1384 1014 957 374 268 247
14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7631
AflIII A'CryG_T
Cuts at: 1203 2013 2647 3929 6727 7704 1203
Size: 810 634 1282 2798 977 1327
Fragments arranged by size:
2798 1327 1282 977 810 634
AgeI A'CCGG_T
Cuts at: 6659 6659
Size: 7828
AhdI GACnn_n'nnGTC
Cuts at: 6557 6557
Size: 7828
AleI CACnn'nnGTG
Cuts at: 1166 4487 1166
Size: 3321 4507
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 3342 1184
Size: 882 1276 5670
Fragments arranged by size:
5670 1276 882
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 7796
AlwNI CAG_nnn'CTG
Cuts at: 3172 5571 7143 3172
Size: 2399 1572 3857
Fragments arranged by size:
3857 2399 1572
ApaLI G'TGCA_C
Cuts at: 5885 7041 5885
Size: 1156 6672
ApoI r'AATT_y
Cuts at: 1493 2089 2608 2743 2960 3250 3578 3737 3748
Size: 596 519 135 217 290 328 159 11
Cuts at: 3748 4386 5137 5363 5515 7624 1493
Size: 638 751 226 152 2109 1697
Fragments arranged by size:
2109 1697 751 638 596 519 328 290
226 217 159 152 135 11
AseI AT'TA_AT
Cuts at: 160 1032 2832 2910 3075 3213 6382 160
Size: 872 1800 78 165 138 3169 1606
Fragments arranged by size:
3169 1800 1606 872 165 138 78
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7828
AvaI C'yCGr_G
Cuts at: 1870 1943 4073 4774 5153 1870
Size: 73 2130 701 379 4545
Fragments arranged by size:
4545 2130 701 379 73
BamHI G'GATC_C
Cuts at: 5158 5525 5158
Size: 367 7461
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5149 6604 618
Size: 325 217 627 3362 1455 1842
Fragments arranged by size:
3362 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5003 5147 1391
Size: 3612 144 4072
Fragments arranged by size:
4072 3612 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 928
Size: 971 510 6347
Fragments arranged by size:
6347 971 510
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4049 4083 6019 6053 1903
Size: 34 2112 34 1936 34 3678
Fragments arranged by size:
3678 2112 1936 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5720 6930 5720
Size: 1210 6618
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7828
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2993 6439 136
Size: 107 122 71 631 605 1321 3446 1525
Fragments arranged by size:
3446 1525 1321 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3682 7823 3682
Size: 4141 3687
BlpI GC'TnA_GC
Cuts at: 5245 5245
Size: 7828
Bme1580I G_kGCm'C
Cuts at: 1790 5889 7045 1790
Size: 4099 1156 2573
Fragments arranged by size:
4099 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3932 1318
Size: 2614 5214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4573 4699 5559
Size: 416 53 83 399 24 3156 126 860
Cuts at: 5559 6517 442
Size: 958 1753
Fragments arranged by size:
3156 1753 958 860 416 399 126 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4040 4475 4964 4994 6488 1239
Size: 2801 435 489 30 1494 2579
Fragments arranged by size:
2801 2579 1494 489 435 30
Bpu10I CC'TnA_GC
Cuts at: 4599 4599
Size: 7828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3543 3796 3902 5953 6818 7116 3543
Size: 253 106 2051 865 298 4255
Fragments arranged by size:
4255 2051 865 298 253 106
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3920 4623 6491 882
Size: 617 2421 703 1868 2219
Fragments arranged by size:
2421 2219 1868 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 2650 4091 5619 493
Size: 711 1446 1441 1528 2702
Fragments arranged by size:
2702 1528 1446 1441 711
BsaBI GATnn'nnATC
Cuts at: 4371 7822 4371
Size: 3451 4377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5635 6017 6652 275
Size: 53 83 186 936 4102 382 635 1451
Fragments arranged by size:
4102 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3357 6261 6659 6933 7080 7491 3357
Size: 2904 398 274 147 411 3694
Fragments arranged by size:
3694 2904 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4937 4967 1178
Size: 30 165 30 3534 30 4039
Fragments arranged by size:
4039 3534 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4772 1186
Size: 3586 4242
BseYI C'CCAG_C
Cuts at: 7031 7527 7031
Size: 496 7332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2170 1163
Size: 573 434 6821
Fragments arranged by size:
6821 573 434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5278 6039 7067 1540
Size: 275 186 3277 761 1028 2301
Fragments arranged by size:
3277 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3652 5003 5889 5974 7045 3652
Size: 1351 886 85 1071 4435
Fragments arranged by size:
4435 1351 1071 886 85
BsmI GAATG_Cn'
Cuts at: 2363 3977 4334 4960 5339 5432 2363
Size: 1614 357 626 379 93 4759
Fragments arranged by size:
4759 1614 626 379 357 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4076 4076
Size: 7828
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3652 5003 5147 5889 5974 7045 1391
Size: 399 1862 1351 144 742 85 1071 2174
Fragments arranged by size:
2174 1862 1351 1071 742 399 144 85
BspEI T'CCGG_A
Cuts at: 3357 7491 3357
Size: 4134 3694
BspHI T'CATG_A
Cuts at: 5717 7447 5717
Size: 1730 6098
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2048 5183 844
Size: 1204 3135 3489
Fragments arranged by size:
3489 3135 1204
BsrBI CCG'CTC
Cuts at: 1394 1823 5715 1394
Size: 429 3892 3507
Fragments arranged by size:
3892 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3116 3522 3559 6323 6497 65
Size: 1183 1868 406 37 2764 174 1396
Fragments arranged by size:
2764 1868 1396 1183 406 174 37
BsrFI r'CCGG_y
Cuts at: 1700 1810 6472 6659 1700
Size: 110 4662 187 2869
Fragments arranged by size:
4662 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2302 96
Size: 2206 5622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7828
BssSI C'ACGA_G
Cuts at: 5888 6900 5888
Size: 1012 6816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7828
BstBI TT'CG_AA
Cuts at: 3398 6632 3398
Size: 3234 4594
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7828
BstYI r'GATC_y
Cuts at: 1972 2953 3682 4989 5158 5525 5910 5927 7379
Size: 981 729 1307 169 367 385 17 1452
Cuts at: 7379 7465 7477 7823 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1307 981 729 385 367 346
169 86 17 12
BstZ17I GTA'TAC
Cuts at: 2330 2330
Size: 7828
Bsu36I CC'TnA_GG
Cuts at: 2384 2900 2384
Size: 516 7312
BtgI C'CryG_G
Cuts at: 513 1065 2004 4524 513
Size: 552 939 2520 3817
Fragments arranged by size:
3817 2520 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7089
Fragments arranged by size:
7089 683 56
BtsI GCAGTG_nn'
Cuts at: 4151 4482 4494 5344 6138 6158 4151
Size: 331 12 850 794 20 5821
Fragments arranged by size:
5821 850 794 331 20 12
ClaI AT'CG_AT
Cuts at: 1554 3231 1554
Size: 1677 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7793
DraI TTT'AAA
Cuts at: 2926 3534 3771 4873 5132 2926
Size: 608 237 1102 259 5622
Fragments arranged by size:
5622 1102 608 259 237
DraIII CAC_nnn'GTG
Cuts at: 3440 3440
Size: 7828
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6835 809
Size: 507 5519 1802
Fragments arranged by size:
5519 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4782 5275 6164
Size: 54 1425 50 275 18 2952 493 889
Cuts at: 6164 7793 8
Size: 1629 43
Fragments arranged by size:
2952 1629 1425 889 493 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5275 1537
Size: 275 3463 4090
Fragments arranged by size:
4090 3463 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4433 4716 5758 1353
Size: 3080 283 1042 3423
Fragments arranged by size:
3423 3080 1042 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6415 6789 6935 387
Size: 1064 138 4826 374 146 1280
Fragments arranged by size:
4826 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5001 5001
Size: 7828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2963 3977 4040 4224 4238 4475
Size: 393 374 957 1014 63 184 14 237
Cuts at: 4475 4964 4994 5891 6488 7275 7543 1239
Size: 489 30 897 597 787 268 1524
Fragments arranged by size:
1524 1014 957 897 787 597 489 393
374 268 237 184 63 30 14
EcoO109I rG'GnC_Cy
Cuts at: 4771 4771
Size: 7828
EcoRI G'AATT_C
Cuts at: 2960 5137 5515 2960
Size: 2177 378 5273
Fragments arranged by size:
5273 2177 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2124 3998 2124
Size: 1874 5954
FspI TGC'GCA
Cuts at: 6334 6334
Size: 7828
HaeII r_GCGC'y
Cuts at: 1678 6975 1678
Size: 5297 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2606 2638 4109 4121 4141
Size: 32 135 32 1034 32 1471 12 20
Cuts at: 4141 4153 4770 4802 6469 6501 6543 6575 1373
Size: 12 617 32 1667 32 42 32 2626
Fragments arranged by size:
2626 1667 1471 1034 617 135 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2162 5172 5418 669
Size: 988 281 224 3010 246 3079
Fragments arranged by size:
3079 3010 988 281 246 224
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7828
HpaI GTT'AAC
Cuts at: 5418 5418
Size: 7828
KpnI G_GTAC'C
Cuts at: 5153 5153
Size: 7828
MfeI C'AATT_G
Cuts at: 5427 5427
Size: 7828
MluI A'CGCG_T
Cuts at: 7704 7704
Size: 7828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3690 4284 6942 7126 1305
Size: 2385 594 2658 184 2007
Fragments arranged by size:
2658 2385 2007 594 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4784 10
Size: 54 1425 343 2952 3054
Fragments arranged by size:
3054 2952 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2800 4017 4377 4487 4523 4529 4922
Size: 648 1634 1217 360 110 36 6 393
Cuts at: 4922 5786 6145 6304 518
Size: 864 359 159 2042
Fragments arranged by size:
2042 1634 1217 864 648 393 360 359
159 110 36 6
MspA1I CmG'CkG
Cuts at: 1606 4987 5244 5921 7069 7314 1606
Size: 3381 257 677 1148 245 2120
Fragments arranged by size:
3381 2120 1148 677 257 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4524 513
Size: 552 939 2520 3817
Fragments arranged by size:
3817 2520 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7828
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7828
NotI GC'GGCC_GC
Cuts at: 5275 5275
Size: 7828
NspI r_CATG'y
Cuts at: 1132 1229 2017 5186 6731 1132
Size: 97 788 3169 1545 2229
Fragments arranged by size:
3169 2229 1545 788 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7828
PciI A'CATG_T
Cuts at: 2013 6727 2013
Size: 4714 3114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 1108
Size: 1077 6751
PfoI T'CCnGG_A
Cuts at: 3141 3141
Size: 7828
PmeI GTTT'AAAC
Cuts at: 5132 5132
Size: 7828
PmlI CAC'GTG
Cuts at: 2650 2650
Size: 7828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5897 5929 7410 7442 5897
Size: 32 1481 32 6283
Fragments arranged by size:
6283 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4771 4771
Size: 7828
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7828
PsiI TTA'TAA
Cuts at: 2513 5398 2513
Size: 2885 4943
PstI C_TGCA'G
Cuts at: 830 4489 5180 830
Size: 3659 691 3478
Fragments arranged by size:
3659 3478 691
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7828
SacI G_AGCT'C
Cuts at: 5003 5003
Size: 7828
SalI G'TCGA_C
Cuts at: 1936 2160 5170 1936
Size: 224 3010 4594
Fragments arranged by size:
4594 3010 224
SanDI GG'GwC_CC
Cuts at: 4771 4771
Size: 7828
SbfI CC_TGCA'GG
Cuts at: 5180 5180
Size: 7828
ScaI AGT'ACT
Cuts at: 6076 7788 6076
Size: 1712 6116
SfcI C'TryA_G
Cuts at: 826 1045 2165 4485 4612 5176 6311 6992 7183
Size: 219 1120 2320 127 564 1135 681 191
Cuts at: 7183 826
Size: 1471
Fragments arranged by size:
2320 1471 1135 1120 681 564 219 191
127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7828
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7828
SmaI CCC'GGG
Cuts at: 1872 4776 5155 1872
Size: 2904 379 4545
Fragments arranged by size:
4545 2904 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3558 3775 3917 4073 5932 6833
Size: 197 926 1615 217 142 156 1859 901
Cuts at: 6833 7095 820
Size: 262 1553
Fragments arranged by size:
1859 1615 1553 926 901 262 217 197
156 142
SnaBI TAC'GTA
Cuts at: 493 5619 493
Size: 5126 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7828
SphI G_CATG'C
Cuts at: 1132 5186 1132
Size: 4054 3774
SspI AAT'ATT
Cuts at: 5 52 3079 3605 5752 5
Size: 47 3027 526 2147 2081
Fragments arranged by size:
3027 2147 2081 526 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7828
StyI C'CwwG_G
Cuts at: 513 1065 2004 4524 5267 513
Size: 552 939 2520 743 3074
Fragments arranged by size:
3074 2520 939 743 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2671 2714 4681 5855 5872 6025 6210
Size: 1255 1091 43 1967 1174 17 153 185
Cuts at: 6210 6655 325
Size: 445 1498
Fragments arranged by size:
1967 1498 1255 1174 1091 445 185 153
43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2302 6074 7786
Size: 274 80 33 51 1443 325 3772 1712
Cuts at: 7786 96
Size: 138
Fragments arranged by size:
3772 1712 1443 325 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7788 7820 7788
Size: 32 7796
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7828
XbaI T'CTAG_A
Cuts at: 2096 3679 3733 4753 5164 2096
Size: 1583 54 1020 411 4760
Fragments arranged by size:
4760 1583 1020 411 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 4187 4531 2426
Size: 1761 344 5723
Fragments arranged by size:
5723 1761 344
XhoI C'TCGA_G
Cuts at: 1943 4073 1943
Size: 2130 5698
XmaI C'CCGG_G
Cuts at: 1870 4774 5153 1870
Size: 2904 379 4545
Fragments arranged by size:
4545 2904 379
XmnI GAAnn'nnTTC
Cuts at: 2250 3513 3741 5957 2250
Size: 1263 228 2216 4121
Fragments arranged by size:
4121 2216 1263 228
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5636 276
Size: 53 83 186 936 4102 2468
Fragments arranged by size:
4102 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI