Restriction map for
pFN21AE5821
(Linear) (Six-base) MAPSORT of: KIEE5821.seq Check: 9676 from: 1 to: 3123
KIEE5821.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AccI GT'mk_AC
Cuts at: 0 156 324 3123
Size: 156 168 2799
Fragments arranged by size:
2799 168 156
AclI AA'CG_TT
Cuts at: 0 2735 3123
Size: 2735 388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 958 1972 2219 2233 3123
Size: 1 957 1014 247 14 890
Fragments arranged by size:
1014 957 890 247 14 1
AflIII A'CryG_T
Cuts at: 0 8 642 1924 3123
Size: 8 634 1282 1199
Fragments arranged by size:
1282 1199 634 8
AleI CACnn'nnGTG
Cuts at: 0 2482 3123
Size: 2482 641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 1337 3123
Size: 61 1276 1786
Fragments arranged by size:
1786 1276 61
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 3123
Size: 27 3096
AlwNI CAG_nnn'CTG
Cuts at: 0 1167 3123
Size: 1167 1956
ApoI r'AATT_y
Cuts at: 0 84 603 738 955 1245 1573 1732 1743
Size: 84 519 135 217 290 328 159 11
Cuts at: 1743 2381 3123
Size: 638 742
Fragments arranged by size:
742 638 519 328 290 217 159 135
84 11
AseI AT'TA_AT
Cuts at: 0 827 905 1070 1208 3123
Size: 827 78 165 138 1915
Fragments arranged by size:
1915 827 165 138 78
AvaI C'yCGr_G
Cuts at: 0 2068 2769 3123
Size: 2068 701 354
Fragments arranged by size:
2068 701 354
BanII G_rGCy'C
Cuts at: 0 2998 3123
Size: 2998 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 404 3123
Size: 404 2719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2044 2078 3123
Size: 2044 34 1045
Fragments arranged by size:
2044 1045 34
BglI GCCn_nnn'nGGC
Cuts at: 0 988 3123
Size: 988 2135
BglII A'GATC_T
Cuts at: 0 1677 3123
Size: 1677 1446
BmgBI CAC'GTC
Cuts at: 0 1927 3123
Size: 1927 1196
BmrI ACTGGGnnnn_n'
Cuts at: 0 2568 2694 3123
Size: 2568 126 429
Fragments arranged by size:
2568 429 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2035 2470 2959 2989 3123
Size: 2035 435 489 30 134
Fragments arranged by size:
2035 489 435 134 30
Bpu10I CC'TnA_GC
Cuts at: 0 2594 3123
Size: 2594 529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 1791 1897 3123
Size: 1538 253 106 1226
Fragments arranged by size:
1538 1226 253 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 1915 2618 3123
Size: 1915 703 505
Fragments arranged by size:
1915 703 505
BsaAI yAC'GTr
Cuts at: 0 645 2086 3123
Size: 645 1441 1037
Fragments arranged by size:
1441 1037 645
BsaBI GATnn'nnATC
Cuts at: 0 2366 3123
Size: 2366 757
BsaWI w'CCGG_w
Cuts at: 0 1352 3123
Size: 1352 1771
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2932 2962 3123
Size: 2932 30 161
Fragments arranged by size:
2932 161 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2767 3123
Size: 2767 356
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 3123
Size: 165 2958
BsiHKAI G_wGCw'C
Cuts at: 0 1647 2998 3123
Size: 1647 1351 125
Fragments arranged by size:
1647 1351 125
BsmI GAATG_Cn'
Cuts at: 0 358 1972 2329 2955 3123
Size: 358 1614 357 626 168
Fragments arranged by size:
1614 626 358 357 168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2071 3123
Size: 2071 1052
Bsp1286I G_dGCh'C
Cuts at: 0 1647 2998 3123
Size: 1647 1351 125
Fragments arranged by size:
1647 1351 125
BspEI T'CCGG_A
Cuts at: 0 1352 3123
Size: 1352 1771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 43 3123
Size: 43 3080
BsrDI GCAATG_nn'
Cuts at: 0 1111 1517 1554 3123
Size: 1111 406 37 1569
Fragments arranged by size:
1569 1111 406 37
BsrGI T'GTAC_A
Cuts at: 0 297 3123
Size: 297 2826
BstBI TT'CG_AA
Cuts at: 0 1393 3123
Size: 1393 1730
BstYI r'GATC_y
Cuts at: 0 948 1677 2984 3123
Size: 948 729 1307 139
Fragments arranged by size:
1307 948 729 139
BstZ17I GTA'TAC
Cuts at: 0 325 3123
Size: 325 2798
Bsu36I CC'TnA_GG
Cuts at: 0 379 895 3123
Size: 379 516 2228
Fragments arranged by size:
2228 516 379
BtgI C'CryG_G
Cuts at: 0 2519 3123
Size: 2519 604
BtsI GCAGTG_nn'
Cuts at: 0 2146 2477 2489 3123
Size: 2146 331 12 634
Fragments arranged by size:
2146 634 331 12
ClaI AT'CG_AT
Cuts at: 0 1226 3123
Size: 1226 1897
DraI TTT'AAA
Cuts at: 0 921 1529 1766 2868 3123
Size: 921 608 237 1102 255
Fragments arranged by size:
1102 921 608 255 237
DraIII CAC_nnn'GTG
Cuts at: 0 1435 3123
Size: 1435 1688
EaeI y'GGCC_r
Cuts at: 0 2777 3123
Size: 2777 346
EarI CTCTTCn'nnn_
Cuts at: 0 2428 2711 3123
Size: 2428 283 412
Fragments arranged by size:
2428 412 283
EcoICRI GAG'CTC
Cuts at: 0 2996 3123
Size: 2996 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 958 1972 2035 2219 2233 2470 2959
Size: 1 957 1014 63 184 14 237 489
Cuts at: 2959 2989 3123
Size: 30 134
Fragments arranged by size:
1014 957 489 237 184 134 63 30
14 1
EcoO109I rG'GnC_Cy
Cuts at: 0 2766 3123
Size: 2766 357
EcoRI G'AATT_C
Cuts at: 0 955 3123
Size: 955 2168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 119 1993 3123
Size: 119 1874 1130
Fragments arranged by size:
1874 1130 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 2104 2116 2136 2148 2765 2797
Size: 601 32 1471 12 20 12 617 32
Cuts at: 2797 3123
Size: 326
Fragments arranged by size:
1471 617 601 326 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 157 3123
Size: 157 2966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1685 2279 3123
Size: 1685 594 844
Fragments arranged by size:
1685 844 594
MscI TGG'CCA
Cuts at: 0 2779 3123
Size: 2779 344
MslI CAynn'nnrTG
Cuts at: 0 795 2012 2372 2482 2518 2524 2917 3123
Size: 795 1217 360 110 36 6 393 206
Fragments arranged by size:
1217 795 393 360 206 110 36 6
MspA1I CmG'CkG
Cuts at: 0 2982 3123
Size: 2982 141
NcoI C'CATG_G
Cuts at: 0 2519 3123
Size: 2519 604
NspI r_CATG'y
Cuts at: 0 12 3123
Size: 12 3111
PciI A'CATG_T
Cuts at: 0 8 3123
Size: 8 3115
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 3123
Size: 180 2943
PfoI T'CCnGG_A
Cuts at: 0 1136 3123
Size: 1136 1987
PmlI CAC'GTG
Cuts at: 0 645 3123
Size: 645 2478
PpuMI rG'GwC_Cy
Cuts at: 0 2766 3123
Size: 2766 357
PsiI TTA'TAA
Cuts at: 0 508 3123
Size: 508 2615
PstI C_TGCA'G
Cuts at: 0 2484 3123
Size: 2484 639
SacI G_AGCT'C
Cuts at: 0 2998 3123
Size: 2998 125
SalI G'TCGA_C
Cuts at: 0 155 3123
Size: 155 2968
SanDI GG'GwC_CC
Cuts at: 0 2766 3123
Size: 2766 357
SfcI C'TryA_G
Cuts at: 0 160 2480 2607 3123
Size: 160 2320 127 516
Fragments arranged by size:
2320 516 160 127
SmaI CCC'GGG
Cuts at: 0 2771 3123
Size: 2771 352
SmlI C'TyrA_G
Cuts at: 0 1553 1770 1912 2068 3123
Size: 1553 217 142 156 1055
Fragments arranged by size:
1553 1055 217 156 142
SspI AAT'ATT
Cuts at: 0 1074 1600 3123
Size: 1074 526 1523
Fragments arranged by size:
1523 1074 526
StyI C'CwwG_G
Cuts at: 0 2519 3123
Size: 2519 604
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 666 709 2676 3123
Size: 666 43 1967 447
Fragments arranged by size:
1967 666 447 43
TatI w'GTAC_w
Cuts at: 0 297 3123
Size: 297 2826
XbaI T'CTAG_A
Cuts at: 0 91 1674 1728 2748 3123
Size: 91 1583 54 1020 375
Fragments arranged by size:
1583 1020 375 91 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 2182 2526 3123
Size: 421 1761 344 597
Fragments arranged by size:
1761 597 421 344
XhoI C'TCGA_G
Cuts at: 0 2068 3123
Size: 2068 1055
XmaI C'CCGG_G
Cuts at: 0 2769 3123
Size: 2769 354
XmnI GAAnn'nnTTC
Cuts at: 0 245 1508 1736 3123
Size: 245 1263 228 1387
Fragments arranged by size:
1387 1263 245 228
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlfI AloI AlwNI
ApoI AseI AvaI BanII BbsI BcgI BglI BglII
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrGI BstBI BstYI BstZ17I Bsu36I
BtgI BtsI ClaI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PciI PflMI PfoI PmlI
PpuMI PsiI PstI SacI SalI SanDI SfcI SmaI
SmlI SspI StyI TaqII TatI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BlpI Bme1580I BmtI BplI
BsaHI BseYI BsiEI BsiWI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstXI BtgZI CspCI DrdI EagI
EciI EcoNI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I ZraI