Restriction map for
pFN21AE3128
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BsrI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MluI MroI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 157 GTCTAC GT^[AC][GT]AC
AccII 52 CGCG CG^CG
AcsI 562 GAATTT [GA]^AATT[CT]
AlwNI 37 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 562 GAATTT [GA]^AATT[CT]
Asp700I 96 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 679 GGCACC G^G[CT][GA]CC
BanII 442 GAGCCC G[GA]GC[CT]^C
BcgI 456 GCAGTGGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 218 TGATCA T^GATCA
BglI 793 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 115 GAGACC GGTCTC(1/5)
BsaWI 608 ACCGGA [AT]^CCGG[AT]
BseRI 208 CTCCTC GAGGAG(10/8)
BsgI 849 CTGCAC GTGCAG(16/14)
BsiEI 60 CGACCG CG[GA][CT]^CG
BsmAI 115 GAGAC GTCTC(1/5)
BspHI 215 TCATGA T^CATGA
BspMI 383 ACCTGC ACCTGC(4/8)
BsrBI 171 GAGCGG CCGCTC(-3/-3)
BsrDI 474 CATTGC GCAATG(2/0)
BssSI 652 CTCGTG CACGAG(-5/-1)
BstAPI 680 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 52 CGCG CG^CG
BstYI 752 GGATCT [GA]^GATC[CT]
CfoI 51 GCGC GCG^C
DdeI 395 CTCAG C^T[ACGT]AG
DraII 38 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 607 GACCGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 508 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 38 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 218 TGATCA T^GATCA
HapII 609 CCGG C^CGG
HhaI 51 GCGC GCG^C
HinP1I 51 GCGC G^CGC
HpaII 609 CCGG C^CGG
MaeII 629 ACGT A^CGT
MfeI 539 CAATTG C^AATTG
MflI 752 GGATCT [GA]^GATC[CT]
MseI 188 TTAA T^TAA
MspI 609 CCGG C^CGG
MunI 539 CAATTG C^AATTG
MvnI 52 CGCG CG^CG
NspI 387 GCATGC [GA]CATG^[CT]
PflMI 48 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 306 GAGTC GAGTC(4/5)
PpuMI 38 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 301 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 454 CTGCAG CTGCA^G
PvuII 713 CAGCTG CAG^CTG
RcaI 215 TCATGA T^CATGA
SfaNI 33 GCATC GCATC(5/9)
SfcI 454 CTGCAG C^T[GA][CT]AG
SphI 387 GCATGC GCATG^C
Tru9I 188 TTAA T^TAA
Van91I 48 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 752 GGATCT [GA]^GATC[CT]
XmnI 96 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BalI 335 TGGCCA TGG^CCA
644 TGGCCA
BmyI 311 GTGCCC G[AGT]GC[ACT]^C
442 GAGCCC
BpmI 73 CTGGAG CTGGAG(16/14)
716 CTGGAG
BsmFI 45 GTCCC GGGAC(10/14)
946 GTCCC
Bsp1286I 311 GTGCCC G[AGT]GC[ACT]^C
442 GAGCCC
BstXI 647 CCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
878 CCATGATCTTGG
DsaI 377 CCATGG C^C[GA][CT]GG
747 CCATGG
EcoT14I 377 CCATGG C^C[AT][AT]GG
747 CCATGG
HgaI 511 GACGC GACGC(5/10)
238 GCGTC
MluNI 335 TGGCCA TGG^CCA
644 TGGCCA
MscI 335 TGGCCA TGG^CCA
644 TGGCCA
MspA1I 21 CAGCGG C[AC]G^C[GT]G
713 CAGCTG
NcoI 377 CCATGG C^CATGG
747 CCATGG
StyI 377 CCATGG C^C[AT][AT]GG
747 CCATGG
Tsp45I 91 GTCAC ^GT[GC]AC
811 GTCAC
TspRI 457 CAGTG CA[GC]TG[ACGT][ACGT]^
847 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I Cfr13I DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I