Restriction map for
pFN21AE3128
(Linear) (Six-base) MAPSORT of: KIEE3128.seq Check: 1792 from: 1 to: 954
KIEE3128.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 158 954
Size: 158 796
AleI CACnn'nnGTG
Cuts at: 0 727 954
Size: 727 227
AlwNI CAG_nnn'CTG
Cuts at: 0 42 954
Size: 42 912
ApoI r'AATT_y
Cuts at: 0 562 954
Size: 562 392
BanI G'GyrC_C
Cuts at: 0 679 954
Size: 679 275
BanII G_rGCy'C
Cuts at: 0 446 954
Size: 446 508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 479 954
Size: 445 34 475
Fragments arranged by size:
475 445 34
BclI T'GATC_A
Cuts at: 0 218 954
Size: 218 736
BglI GCCn_nnn'nGGC
Cuts at: 0 799 954
Size: 799 155
Bme1580I G_kGCm'C
Cuts at: 0 315 954
Size: 315 639
BmgBI CAC'GTC
Cuts at: 0 630 954
Size: 630 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 737 954
Size: 94 643 217
Fragments arranged by size:
643 217 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 954
Size: 109 845
BsaWI w'CCGG_w
Cuts at: 0 608 954
Size: 608 346
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 954
Size: 199 755
BseYI C'CCAG_C
Cuts at: 0 47 78 355 954
Size: 47 31 277 599
Fragments arranged by size:
599 277 47 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 954
Size: 834 120
BsiEI CG_ry'CG
Cuts at: 0 63 954
Size: 63 891
Bsp1286I G_dGCh'C
Cuts at: 0 315 446 954
Size: 315 131 508
Fragments arranged by size:
508 315 131
BspHI T'CATG_A
Cuts at: 0 215 954
Size: 215 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 954
Size: 392 562
BsrBI CCG'CTC
Cuts at: 0 173 954
Size: 173 781
BsrDI GCAATG_nn'
Cuts at: 0 473 954
Size: 473 481
BssSI C'ACGA_G
Cuts at: 0 652 954
Size: 652 302
BstAPI GCAn_nnn'nTGC
Cuts at: 0 686 954
Size: 686 268
BstXI CCAn_nnnn'nTGG
Cuts at: 0 654 885 954
Size: 654 231 69
Fragments arranged by size:
654 231 69
BstYI r'GATC_y
Cuts at: 0 752 954
Size: 752 202
BtgI C'CryG_G
Cuts at: 0 377 747 954
Size: 377 370 207
Fragments arranged by size:
377 370 207
BtsI GCAGTG_nn'
Cuts at: 0 463 846 954
Size: 463 383 108
Fragments arranged by size:
463 383 108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 314 349 954
Size: 314 35 605
Fragments arranged by size:
605 314 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 613 954
Size: 613 341
EaeI y'GGCC_r
Cuts at: 0 56 335 644 887 954
Size: 56 279 309 243 67
Fragments arranged by size:
309 279 243 67 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 376 954
Size: 376 578
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 737 954
Size: 94 643 217
Fragments arranged by size:
643 217 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 512 954
Size: 512 442
EcoO109I rG'GnC_Cy
Cuts at: 0 39 954
Size: 39 915
MfeI C'AATT_G
Cuts at: 0 539 954
Size: 539 415
MscI TGG'CCA
Cuts at: 0 337 646 954
Size: 337 309 308
Fragments arranged by size:
337 309 308
MslI CAynn'nnrTG
Cuts at: 0 376 652 727 877 954
Size: 376 276 75 150 77
Fragments arranged by size:
376 276 150 77 75
MspA1I CmG'CkG
Cuts at: 0 23 715 954
Size: 23 692 239
Fragments arranged by size:
692 239 23
NcoI C'CATG_G
Cuts at: 0 377 747 954
Size: 377 370 207
Fragments arranged by size:
377 370 207
NspI r_CATG'y
Cuts at: 0 391 954
Size: 391 563
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 954
Size: 54 900
PpuMI rG'GwC_Cy
Cuts at: 0 39 954
Size: 39 915
PshAI GACnn'nnGTC
Cuts at: 0 305 954
Size: 305 649
PstI C_TGCA'G
Cuts at: 0 458 954
Size: 458 496
PvuII CAG'CTG
Cuts at: 0 715 954
Size: 715 239
SfcI C'TryA_G
Cuts at: 0 454 954
Size: 454 500
SphI G_CATG'C
Cuts at: 0 391 954
Size: 391 563
StyI C'CwwG_G
Cuts at: 0 377 747 954
Size: 377 370 207
Fragments arranged by size:
377 370 207
XmnI GAAnn'nnTTC
Cuts at: 0 100 954
Size: 100 854
Enzymes that do cut:
AccI AleI AlwNI ApoI BanI BanII BcgI BclI
BglI Bme1580I BmgBI BpmI BsaI BsaWI BseRI BseYI
BsgI BsiEI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI
BstAPI BstXI BstYI BtgI BtsI CspCI DrdI EaeI
EciI Eco57MI EcoNI EcoO109I MfeI MscI MslI MspA1I
NcoI NspI PflMI PpuMI PshAI PstI PvuII SfcI
SphI StyI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI