Restriction map for
pFN21AE6607
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# NO CUT ENZYMES ( 166 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 730 GACGTC GACGT^C
AccI 300 GTCTAC GT^[AC][GT]AC
AccII 175 CGCG CG^CG
AcyI 730 GACGTC G[GA]^CG[CT]C
AfaI 186 GTAC GT^AC
AluI 276 AGCT AG^CT
BbvI 517 GCTGC GCAGC(8/12)
BfaI 235 CTAG C^TAG
BpmI 278 CTCCAG CTGGAG(16/14)
BsaAI 187 TACGTG [CT]AC^GT[GA]
BsaHI 730 GACGTC G[GA]^CG[CT]C
BsaJI 580 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 548 TCCGGT [AT]^CCGG[AT]
BsmAI 9 GTCTC GTCTC(1/5)
BsmI 381 GAATGC GAATGC(1/-1)
BsrDI 385 GCAATG GCAATG(2/0)
BstUI 175 CGCG CG^CG
BstYI 237 AGATCC [GA]^GATC[CT]
CfoI 176 GCGC GCG^C
EarI 313 GAAGAG CTCTTC(1/4)
EcoRV 421 GATATC GAT^ATC
EcoT14I 580 CCAAGG C^C[AT][AT]GG
EcoT22I 79 ATGCAT ATGCA^T
FauI 653 GCGGG CCCGC(4/6)
HapII 549 CCGG C^CGG
HgaI 55 GCGTC GACGC(5/10)
HhaI 176 GCGC GCG^C
Hin1I 730 GACGTC G[GA]^CG[CT]C
HinP1I 176 GCGC G^CGC
HincII 700 GTCAAC GT[CT]^[GA]AC
HindII 700 GTCAAC GT[CT]^[GA]AC
HpaII 549 CCGG C^CGG
Ksp632I 313 GAAGAG CTCTTC(1/4)
MaeI 235 CTAG C^TAG
MflI 237 AGATCC [GA]^GATC[CT]
MseI 391 TTAA T^TAA
MspI 549 CCGG C^CGG
MvnI 175 CGCG CG^CG
NsiI 79 ATGCAT ATGCA^T
PleI 316 GAGTC GAGTC(4/5)
PshAI 730 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 518 CTGCAG CTGCA^G
RsaI 186 GTAC GT^AC
SfcI 518 CTGCAG C^T[GA][CT]AG
StuI 38 AGGCCT AGG^CCT
StyI 580 CCAAGG C^C[AT][AT]GG
Tru9I 391 TTAA T^TAA
TseI 517 GCTGC G^C[AT]GC
XhoII 237 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 214 GCGG CCGC(-3/-1)
653 GCGG
AcsI 455 GAATTC [GA]^AATT[CT]
590 GAATTC
ApoI 455 GAATTC [GA]^AATT[CT]
590 GAATTC
Asp700I 505 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
590 GAATTCGTTC
AvaII 535 GGTCC G^G[AT]CC
797 GGACC
BmyI 207 GGGCAC G[AGT]GC[ACT]^C
584 GGGCAC
Bsp1286I 207 GGGCAC G[AGT]GC[ACT]^C
584 GGGCAC
BsrI 106 CCAGT ACTGG(1/-1)
714 CCAGT
Cac8I 159 GCCTGC GC[ACGT]^[ACGT]GC
477 GCAGGC
EcoRI 455 GAATTC G^AATTC
590 GAATTC
Fnu4HI 214 GCGGC GC^[ACGT]GC
517 GCTGC
HaeIII 39 GGCC GG^CC
127 GGCC
ItaI 214 GCGGC GC^[ACGT]GC
517 GCTGC
MaeII 188 ACGT A^CGT
731 ACGT
MboII 141 GAAGA GAAGA(8/7)
313 GAAGA
NlaIII 82 CATG CATG^
514 CATG
TfiI 337 GATTC G^A[AT]TC
748 GATTC
Tsp509I 456 AATT ^AATT
591 AATT
TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^
210 CACTG
XmnI 505 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
590 GAATTCGTTC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AlwI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII FokI HinfI HphI MboI
MnlI MvaI MwoI NdeII Sau3AI
Sau96I ScrFI SfaNI