################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AccI AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI FbaI FseI FspI HincII HindII HindIII HpaI KasI KspI MamI MluI NarI NcoI NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1792 GACGTC GACGT^C Acc65I 1739 GGTACC G^GTACC AccIII 2150 TCCGGA T^CCGGA AcyI 1792 GACGTC G[GA]^CG[CT]C Asp700I 1957 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1739 GGTACC G^GTACC AvaI 2041 CCCGAG C^[CT]CG[GA]G BalI 1784 TGGCCA TGG^CCA BbrPI 685 CACGTG CAC^GTG BbvCI 2094 CCTCAGC CCTCAGC(-5/-2) BcgI 1847 GCATGACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 685 CACGTG [CT]AC^GT[GA] BsaHI 1792 GACGTC G[GA]^CG[CT]C BseAI 2150 TCCGGA T^CCGGA BseRI 1950 GAGGAG GAGGAG(10/8) BsiEI 1294 CGGCCG CG[GA][CT]^CG BsoBI 2041 CCCGAG C^[CT]CG[GA]G Bsp1407I 464 TGTACA T^GTACA BspDI 262 ATCGAT AT^CGAT BspEI 2150 TCCGGA T^CCGGA BsrGI 464 TGTACA T^GTACA ClaI 262 ATCGAT AT^CGAT DraII 2255 AGGACCC [GA]G^G[ACGT]CC[CT] EagI 1294 CGGCCG C^GGCCG EclXI 1294 CGGCCG C^GGCCG Eco52I 1294 CGGCCG C^GGCCG EcoO109I 2255 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 2143 GATATC GAT^ATC EcoT22I 1691 ATGCAT ATGCA^T Hin1I 1792 GACGTC G[GA]^CG[CT]C KpnI 1739 GGTACC GGTAC^C MfeI 812 CAATTG C^AATTG MluNI 1784 TGGCCA TGG^CCA MroI 2150 TCCGGA T^CCGGA MscI 1784 TGGCCA TGG^CCA MunI 812 CAATTG C^AATTG NsiI 1691 ATGCAT ATGCA^T PmaCI 685 CACGTG CAC^GTG PmlI 685 CACGTG CAC^GTG PpuMI 2255 AGGACCC [GA]G^G[AT]CC[CT] PshAI 379 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 773 CTGCAG CTGCA^G SacI 1186 GAGCTC GAGCT^C SfcI 773 CTGCAG C^T[GA][CT]AG SmlI 1222 CTTGAG C^T[CT][GA]AG SspBI 464 TGTACA T^GTACA XbaI 834 TCTAGA T^CTAGA XmnI 1957 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 1428 CGCG CG^CG 2183 CGCG Alw44I 682 GTGCAC G^TGCAC 806 GTGCAC ApaLI 682 GTGCAC G^TGCAC 806 GTGCAC AspI 1525 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1593 GACGGTGTC BanII 1186 GAGCTC G[GA]GC[CT]^C 1217 GAGCCC BfaI 679 CTAG C^TAG 835 CTAG BglI 892 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2239 GCCCAGATGGC BsaWI 649 TCCGGT [AT]^CCGG[AT] 2150 TCCGGA BsmBI 943 CGTCTC CGTCTC(1/5) 1867 GAGACG BsrBI 570 GAGCGG CCGCTC(-3/-3) 2187 GAGCGG BsrDI 1146 CATTGC GCAATG(2/0) 1549 GCAATG BstAPI 523 GCACCATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 775 GCAGCGGCTGC BstUI 1428 CGCG CG^CG 2183 CGCG BstXI 1085 CCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1728 CCACTTAAATGG BstYI 916 AGATCC [GA]^GATC[CT] 1388 AGATCC DsaI 272 CCGTGG C^C[GA][CT]GG 1904 CCGTGG EcoT14I 248 CCTTGG C^C[AT][AT]GG 660 CCTTGG HaeII 477 GGCGCT [GA]GCGC^[CT] 1928 AGCGCC HgaI 1869 GACGC GACGC(5/10) 2181 GACGC MaeI 679 CTAG C^TAG 835 CTAG MflI 916 AGATCC [GA]^GATC[CT] 1388 AGATCC MvnI 1428 CGCG CG^CG 2183 CGCG NaeI 1296 GCCGGC GCC^GGC 2049 GCCGGC NgoMIV 1296 GCCGGC G^CCGGC 2049 GCCGGC PflFI 1525 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1593 GACGGTGTC PflMI 95 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1673 CCAGCAAGTGG StyI 248 CCTTGG C^C[AT][AT]GG 660 CCTTGG Tth111I 1525 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1593 GACGGTGTC Van91I 95 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1673 CCAGCAAGTGG XcmI 1322 CCATGTGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1412 CCAGCATGGCTGTGG XhoII 916 AGATCC [GA]^GATC[CT] 1388 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoNI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I