(Circular) (Six-base) MAPSORT of: pFN21AE5737.seq Check: 7100 from: 1 to: 6985 pFN21AE5737 6985 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3801 4795 278 Size: 53 83 186 936 2265 994 2468 Fragments arranged by size: 2468 2265 994 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4328 1937 Size: 2391 4594 Acc65I G'GTAC_C Cuts at: 3744 4306 3744 Size: 562 6423 AclI AA'CG_TT Cuts at: 1563 5112 5485 1563 Size: 3549 373 3063 Fragments arranged by size: 3549 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2318 2701 2888 3487 4097 5048 6432 6700 Size: 686 383 187 599 610 951 1384 268 Cuts at: 6700 1632 Size: 1917 Fragments arranged by size: 1917 1384 951 686 610 599 383 268 187 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6788 AflIII A'CryG_T Cuts at: 1203 5884 6861 1203 Size: 4681 977 1327 Fragments arranged by size: 4681 1327 977 AgeI A'CCGG_T Cuts at: 5816 5816 Size: 6985 AhdI GACnn_n'nnGTC Cuts at: 5714 5714 Size: 6985 AleI CACnn'nnGTG Cuts at: 1166 2636 1166 Size: 1470 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6985 AlwNI CAG_nnn'CTG Cuts at: 2786 3510 4180 4728 6300 2786 Size: 724 670 548 1572 3471 Fragments arranged by size: 3471 1572 724 670 548 ApaLI G'TGCA_C Cuts at: 2687 2811 5042 6198 2687 Size: 124 2231 1156 3474 Fragments arranged by size: 3474 2231 1156 124 ApoI r'AATT_y Cuts at: 1493 4294 4520 4672 6781 1493 Size: 2801 226 152 2109 1697 Fragments arranged by size: 2801 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5539 160 Size: 872 4507 1606 Fragments arranged by size: 4507 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 AvaI C'yCGr_G Cuts at: 1870 1943 4046 4310 1870 Size: 73 2103 264 4545 Fragments arranged by size: 4545 2103 264 73 BamHI G'GATC_C Cuts at: 4315 4682 4315 Size: 367 6618 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2438 3744 4306 5761 Size: 325 217 627 462 189 1306 562 1455 Cuts at: 5761 618 Size: 1842 Fragments arranged by size: 1842 1455 1306 627 562 462 325 217 189 BanII G_rGCy'C Cuts at: 1391 3195 3226 4304 1391 Size: 1804 31 1078 4072 Fragments arranged by size: 4072 1804 1078 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2876 3016 3225 928 Size: 971 977 140 209 4688 Fragments arranged by size: 4688 977 971 209 140 BbvCI CC'TCA_GC Cuts at: 4100 4100 Size: 6985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 5176 5210 1903 Size: 34 1904 34 1301 34 3678 Fragments arranged by size: 3678 1904 1301 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4877 6087 4877 Size: 1210 5775 BclI T'GATC_A Cuts at: 1548 1548 Size: 6985 BfrBI ATG'CAT Cuts at: 3698 3698 Size: 6985 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 4250 5596 Size: 107 122 71 631 605 1231 1347 1346 Cuts at: 5596 136 Size: 1525 Fragments arranged by size: 1525 1347 1346 1231 631 605 122 107 71 BglII A'GATC_T Cuts at: 6980 6980 Size: 6985 BlpI GC'TnA_GC Cuts at: 4402 4402 Size: 6985 Bme1580I G_kGCm'C Cuts at: 1790 2252 2691 2815 3657 5046 6202 1790 Size: 462 439 124 842 1389 1156 2573 Fragments arranged by size: 2573 1389 1156 842 462 439 124 BmgBI CAC'GTC Cuts at: 1318 3760 3868 1318 Size: 2442 108 4435 Fragments arranged by size: 4435 2442 108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4172 4716 5674 Size: 416 53 83 399 24 2755 544 958 Cuts at: 5674 442 Size: 1753 Fragments arranged by size: 2755 1753 958 544 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2811 3137 3662 3904 5645 1239 Size: 1572 326 525 242 1741 2579 Fragments arranged by size: 2579 1741 1572 525 326 242 Bpu10I CC'TnA_GC Cuts at: 2111 4100 2111 Size: 1989 4996 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3248 5110 5975 6273 3248 Size: 1862 865 298 3960 Fragments arranged by size: 3960 1862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 3881 3922 5648 882 Size: 617 858 1524 41 1726 2219 Fragments arranged by size: 2219 1726 1524 858 617 41 BsaAI yAC'GTr Cuts at: 493 1204 2692 4776 493 Size: 711 1488 2084 2702 Fragments arranged by size: 2702 2084 1488 711 BsaBI GATnn'nnATC Cuts at: 6979 6979 Size: 6985 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3798 4792 5174 5809 Size: 53 83 186 936 2265 994 382 635 Cuts at: 5809 275 Size: 1451 Fragments arranged by size: 2265 1451 994 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2654 4155 5418 5816 6090 6237 6648 2654 Size: 1501 1263 398 274 147 411 2991 Fragments arranged by size: 2991 1501 1263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2784 2814 1178 Size: 30 165 30 1381 30 5349 Fragments arranged by size: 5349 1381 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3970 1186 Size: 2784 4201 BseYI C'CCAG_C Cuts at: 2258 3097 3237 3416 6188 6684 2258 Size: 839 140 179 2772 496 2559 Fragments arranged by size: 2772 2559 839 496 179 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3094 3535 3546 1163 Size: 573 1358 441 11 4602 Fragments arranged by size: 4602 1358 573 441 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3302 4435 5196 6224 1540 Size: 275 186 1301 1133 761 1028 2301 Fragments arranged by size: 2301 1301 1133 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 2815 3185 3195 3840 5046 5131 6202 2691 Size: 124 370 10 645 1206 85 1071 3474 Fragments arranged by size: 3474 1206 1071 645 370 124 85 10 BsmI GAATG_Cn' Cuts at: 4496 4589 4496 Size: 93 6892 BsmBI CGTCTCn'nnnn_ Cuts at: 2954 3866 2954 Size: 912 6073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2252 2691 2815 3185 3195 3226 3657 Size: 399 462 439 124 370 10 31 431 Cuts at: 3657 3840 4304 5046 5131 6202 1391 Size: 183 464 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 464 462 439 431 399 370 183 124 85 31 10 BspEI T'CCGG_A Cuts at: 4155 6648 4155 Size: 2493 4492 BspHI T'CATG_A Cuts at: 4874 6604 4874 Size: 1730 5255 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4340 844 Size: 3496 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2577 4194 4872 1394 Size: 429 754 1617 678 3507 Fragments arranged by size: 3507 1617 754 678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3150 3561 5480 5654 65 Size: 1183 1902 411 1919 174 1396 Fragments arranged by size: 1919 1902 1396 1183 411 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3297 3301 4054 5629 5816 1700 Size: 110 1487 4 753 1575 187 2869 Fragments arranged by size: 2869 1575 1487 753 187 110 4 BsrGI T'GTAC_A Cuts at: 96 2469 96 Size: 2373 4612 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6985 BssSI C'ACGA_G Cuts at: 5045 6057 5045 Size: 1012 5973 BstAPI GCAn_nnn'nTGC Cuts at: 794 2534 2786 794 Size: 1740 252 4993 Fragments arranged by size: 4993 1740 252 BstBI TT'CG_AA Cuts at: 5789 5789 Size: 6985 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6985 BstXI CCAn_nnnn'nTGG Cuts at: 3097 3740 3097 Size: 643 6342 BstYI r'GATC_y Cuts at: 1972 2921 3393 4315 4682 5067 5084 6536 6622 Size: 949 472 922 367 385 17 1452 86 Cuts at: 6622 6634 6980 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 949 922 472 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2277 3909 513 Size: 552 939 273 1632 3589 Fragments arranged by size: 3589 1632 939 552 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2036 3049 487 Size: 683 56 810 1013 4423 Fragments arranged by size: 4423 1013 810 683 56 BtsI GCAGTG_nn' Cuts at: 2348 3141 4181 4501 5295 5315 2348 Size: 793 1040 320 794 20 4018 Fragments arranged by size: 4018 1040 794 793 320 20 ClaI AT'CG_AT Cuts at: 1554 2268 1554 Size: 714 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3199 3234 529 Size: 35 2635 35 4280 Fragments arranged by size: 4280 2635 35 35 DraI TTT'AAA Cuts at: 4289 4289 Size: 6985 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5992 809 Size: 507 4676 1802 Fragments arranged by size: 4676 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3299 3324 3789 Size: 54 1425 50 275 18 1469 25 465 Cuts at: 3789 4056 4432 5321 6950 8 Size: 267 376 889 1629 43 Fragments arranged by size: 1629 1469 1425 889 465 376 275 267 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3299 4432 1537 Size: 275 1487 1133 4090 Fragments arranged by size: 4090 1487 1133 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2838 3005 3053 3413 4915 1353 Size: 1485 167 48 360 1502 3423 Fragments arranged by size: 3423 1502 1485 360 167 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5572 5946 6092 387 Size: 1064 138 3983 374 146 1280 Fragments arranged by size: 3983 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3193 3193 Size: 6985 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 2701 2811 2888 3137 3487 3662 Size: 393 686 383 110 77 249 350 175 Cuts at: 3662 3904 4097 5048 5645 6432 6700 1239 Size: 242 193 951 597 787 268 1524 Fragments arranged by size: 1524 951 787 686 597 393 383 350 268 249 242 193 175 110 77 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2669 2878 3073 2300 Size: 369 209 195 6212 Fragments arranged by size: 6212 369 209 195 EcoO109I rG'GnC_Cy Cuts at: 4261 4261 Size: 6985 EcoRI G'AATT_C Cuts at: 4294 4672 4294 Size: 378 6607 EcoRV GAT'ATC Cuts at: 4150 4150 Size: 6985 FspI TGC'GCA Cuts at: 5491 5491 Size: 6985 HaeII r_GCGC'y Cuts at: 1678 2486 3937 6132 1678 Size: 808 1451 2195 2531 Fragments arranged by size: 2531 2195 1451 808 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 4253 4285 5626 Size: 32 135 32 620 32 2029 32 1341 Cuts at: 5626 5658 5700 5732 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2029 1341 620 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4329 4575 669 Size: 988 281 2391 246 3079 Fragments arranged by size: 3079 2391 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6985 HpaI GTT'AAC Cuts at: 4575 4575 Size: 6985 KpnI G_GTAC'C Cuts at: 3748 4310 3748 Size: 562 6423 MfeI C'AATT_G Cuts at: 2817 4584 2817 Size: 1767 5218 MluI A'CGCG_T Cuts at: 6861 6861 Size: 6985 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 6099 6283 1305 Size: 1086 3708 184 2007 Fragments arranged by size: 3708 2007 1086 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3791 10 Size: 54 1425 343 1959 3204 Fragments arranged by size: 3204 1959 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2636 3128 3326 3425 3486 3738 4130 Size: 648 1470 492 198 99 61 252 392 Cuts at: 4130 4943 5302 5461 518 Size: 813 359 159 2042 Fragments arranged by size: 2042 1470 813 648 492 392 359 252 198 159 99 61 MspA1I CmG'CkG Cuts at: 1606 2777 2783 3057 3337 3472 4401 5078 6226 Size: 1171 6 274 280 135 929 677 1148 Cuts at: 6226 6471 1606 Size: 245 2120 Fragments arranged by size: 2120 1171 1148 929 677 280 274 245 135 6 NaeI GCC'GGC Cuts at: 3303 4056 3303 Size: 753 6232 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5494 Fragments arranged by size: 5494 939 552 NdeI CA'TA_TG Cuts at: 387 2174 2533 4239 387 Size: 1787 359 1706 3133 Fragments arranged by size: 3133 1787 1706 359 NgoMIV G'CCGG_C Cuts at: 3301 4054 3301 Size: 753 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6985 NotI GC'GGCC_GC Cuts at: 4432 4432 Size: 6985 NsiI A_TGCA'T Cuts at: 3700 3700 Size: 6985 NspI r_CATG'y Cuts at: 1132 1229 4343 5888 1132 Size: 97 3114 1545 2229 Fragments arranged by size: 3114 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6985 PciI A'CATG_T Cuts at: 5884 5884 Size: 6985 PflMI CCAn_nnn'nTGG Cuts at: 1108 2106 3684 1108 Size: 998 1578 4409 Fragments arranged by size: 4409 1578 998 PmeI GTTT'AAAC Cuts at: 4289 4289 Size: 6985 PmlI CAC'GTG Cuts at: 2692 2692 Size: 6985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3954 3986 5054 5086 6567 6599 3954 Size: 32 1068 32 1481 32 4340 Fragments arranged by size: 4340 1481 1068 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4261 4261 Size: 6985 PshAI GACnn'nnGTC Cuts at: 1613 2388 1613 Size: 775 6210 PsiI TTA'TAA Cuts at: 4555 4555 Size: 6985 PstI C_TGCA'G Cuts at: 830 2782 4337 830 Size: 1952 1555 3478 Fragments arranged by size: 3478 1952 1555 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6985 PvuII CAG'CTG Cuts at: 2777 3337 3472 2777 Size: 560 135 6290 Fragments arranged by size: 6290 560 135 SacI G_AGCT'C Cuts at: 3195 3195 Size: 6985 SalI G'TCGA_C Cuts at: 1936 4327 1936 Size: 2391 4594 SbfI CC_TGCA'GG Cuts at: 4337 4337 Size: 6985 ScaI AGT'ACT Cuts at: 5233 6945 5233 Size: 1712 5273 SfcI C'TryA_G Cuts at: 826 1045 2778 4333 5468 6149 6340 826 Size: 219 1733 1555 1135 681 191 1471 Fragments arranged by size: 1733 1555 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6985 SmaI CCC'GGG Cuts at: 1872 4312 1872 Size: 2440 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3227 5089 5990 6252 820 Size: 197 926 1284 1862 901 262 1553 Fragments arranged by size: 1862 1553 1284 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4776 493 Size: 4283 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6985 SphI G_CATG'C Cuts at: 1132 4343 1132 Size: 3211 3774 SspI AAT'ATT Cuts at: 5 52 4909 5 Size: 47 4857 2081 Fragments arranged by size: 4857 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6985 StyI C'CwwG_G Cuts at: 513 1065 2004 2253 2665 4424 513 Size: 552 939 249 412 1759 3074 Fragments arranged by size: 3074 1759 939 552 412 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2206 3857 3905 5012 5029 5182 5367 Size: 1255 626 1651 48 1107 17 153 185 Cuts at: 5367 5812 325 Size: 445 1498 Fragments arranged by size: 1651 1498 1255 1107 626 445 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2469 2736 3645 Size: 274 80 33 51 1443 492 267 909 Cuts at: 3645 4026 5231 6943 96 Size: 381 1205 1712 138 Fragments arranged by size: 1712 1443 1205 909 492 381 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 3447 3479 6945 6977 2205 Size: 32 1210 32 3466 32 2213 Fragments arranged by size: 3466 2213 1210 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3533 3601 1316 Size: 2217 68 4700 Fragments arranged by size: 4700 2217 68 XbaI T'CTAG_A Cuts at: 2839 4321 2839 Size: 1482 5503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3334 3424 3334 Size: 90 6895 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6985 XmaI C'CCGG_G Cuts at: 1870 4310 1870 Size: 2440 4545 XmnI GAAnn'nnTTC Cuts at: 3966 5114 3966 Size: 1148 5837 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3799 4793 276 Size: 53 83 186 936 2265 994 2468 Fragments arranged by size: 2468 2265 994 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI