(Linear) (Six-base) MAPSORT of: KIEE5737.seq Check: 399 from: 1 to: 2280 KIEE5737.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AatII G_ACGT'C Cuts at: 0 1796 2280 Size: 1796 484 Acc65I G'GTAC_C Cuts at: 0 1739 2280 Size: 1739 541 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 696 883 1482 2092 2280 Size: 313 383 187 599 610 188 Fragments arranged by size: 610 599 383 313 188 187 AleI CACnn'nnGTG Cuts at: 0 631 2280 Size: 631 1649 AlwNI CAG_nnn'CTG Cuts at: 0 781 1505 2175 2280 Size: 781 724 670 105 Fragments arranged by size: 781 724 670 105 ApaLI G'TGCA_C Cuts at: 0 682 806 2280 Size: 682 124 1474 Fragments arranged by size: 1474 682 124 AvaI C'yCGr_G Cuts at: 0 2041 2280 Size: 2041 239 BanI G'GyrC_C Cuts at: 0 244 433 1739 2280 Size: 244 189 1306 541 Fragments arranged by size: 1306 541 244 189 BanII G_rGCy'C Cuts at: 0 1190 1221 2280 Size: 1190 31 1059 Fragments arranged by size: 1190 1059 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 871 1011 1220 2280 Size: 871 140 209 1060 Fragments arranged by size: 1060 871 209 140 BbvCI CC'TCA_GC Cuts at: 0 2095 2280 Size: 2095 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1836 1870 2280 Size: 1836 34 410 Fragments arranged by size: 1836 410 34 BfrBI ATG'CAT Cuts at: 0 1693 2280 Size: 1693 587 BglI GCCn_nnn'nGGC Cuts at: 0 898 2245 2280 Size: 898 1347 35 Fragments arranged by size: 1347 898 35 Bme1580I G_kGCm'C Cuts at: 0 247 686 810 1652 2280 Size: 247 439 124 842 628 Fragments arranged by size: 842 628 439 247 124 BmgBI CAC'GTC Cuts at: 0 1755 1863 2280 Size: 1755 108 417 Fragments arranged by size: 1755 417 108 BmrI ACTGGGnnnn_n' Cuts at: 0 2167 2280 Size: 2167 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 806 1132 1657 1899 2280 Size: 806 326 525 242 381 Fragments arranged by size: 806 525 381 326 242 Bpu10I CC'TnA_GC Cuts at: 0 106 2095 2280 Size: 106 1989 185 Fragments arranged by size: 1989 185 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1243 2280 Size: 1243 1037 BsaI GGTCTCn'nnnn_ Cuts at: 0 352 1876 1917 2280 Size: 352 1524 41 363 Fragments arranged by size: 1524 363 352 41 BsaAI yAC'GTr Cuts at: 0 687 2280 Size: 687 1593 BsaHI Gr'CG_yC Cuts at: 0 1793 2280 Size: 1793 487 BsaWI w'CCGG_w Cuts at: 0 649 2150 2280 Size: 649 1501 130 Fragments arranged by size: 1501 649 130 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 779 809 2280 Size: 779 30 1471 Fragments arranged by size: 1471 779 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1965 2280 Size: 1965 315 BseYI C'CCAG_C Cuts at: 0 253 1092 1232 1411 2280 Size: 253 839 140 179 869 Fragments arranged by size: 869 839 253 179 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1089 1530 1541 2280 Size: 1089 441 11 739 Fragments arranged by size: 1089 739 441 11 BsiEI CG_ry'CG Cuts at: 0 1297 2280 Size: 1297 983 BsiHKAI G_wGCw'C Cuts at: 0 686 810 1180 1190 1835 2280 Size: 686 124 370 10 645 445 Fragments arranged by size: 686 645 445 370 124 10 BsmBI CGTCTCn'nnnn_ Cuts at: 0 949 1861 2280 Size: 949 912 419 Fragments arranged by size: 949 912 419 Bsp1286I G_dGCh'C Cuts at: 0 247 686 810 1180 1190 1221 1652 1835 Size: 247 439 124 370 10 31 431 183 Cuts at: 1835 2280 Size: 445 Fragments arranged by size: 445 439 431 370 247 183 124 31 10 BspEI T'CCGG_A Cuts at: 0 2150 2280 Size: 2150 130 BsrBI CCG'CTC Cuts at: 0 572 2189 2280 Size: 572 1617 91 Fragments arranged by size: 1617 572 91 BsrDI GCAATG_nn' Cuts at: 0 1145 1556 2280 Size: 1145 411 724 Fragments arranged by size: 1145 724 411 BsrFI r'CCGG_y Cuts at: 0 1292 1296 2049 2280 Size: 1292 4 753 231 Fragments arranged by size: 1292 753 231 4 BsrGI T'GTAC_A Cuts at: 0 464 2280 Size: 464 1816 BstAPI GCAn_nnn'nTGC Cuts at: 0 529 781 2280 Size: 529 252 1499 Fragments arranged by size: 1499 529 252 BstXI CCAn_nnnn'nTGG Cuts at: 0 1092 1735 2280 Size: 1092 643 545 Fragments arranged by size: 1092 643 545 BstYI r'GATC_y Cuts at: 0 916 1388 2280 Size: 916 472 892 Fragments arranged by size: 916 892 472 BtgI C'CryG_G Cuts at: 0 272 1904 2280 Size: 272 1632 376 Fragments arranged by size: 1632 376 272 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 31 1044 2280 Size: 31 1013 1236 Fragments arranged by size: 1236 1013 31 BtsI GCAGTG_nn' Cuts at: 0 343 1136 2176 2280 Size: 343 793 1040 104 Fragments arranged by size: 1040 793 343 104 ClaI AT'CG_AT Cuts at: 0 263 2280 Size: 263 2017 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1194 1229 2280 Size: 1194 35 1051 Fragments arranged by size: 1194 1051 35 EaeI y'GGCC_r Cuts at: 0 1294 1319 1784 2051 2280 Size: 1294 25 465 267 229 Fragments arranged by size: 1294 465 267 229 25 EagI C'GGCC_G Cuts at: 0 1294 2280 Size: 1294 986 EarI CTCTTCn'nnn_ Cuts at: 0 833 1000 1048 1408 2280 Size: 833 167 48 360 872 Fragments arranged by size: 872 833 360 167 48 EcoICRI GAG'CTC Cuts at: 0 1188 2280 Size: 1188 1092 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 313 696 806 883 1132 1482 1657 1899 Size: 313 383 110 77 249 350 175 242 Cuts at: 1899 2092 2280 Size: 193 188 Fragments arranged by size: 383 350 313 249 242 193 188 175 110 77 EcoNI CCTnn'n_nnAGG Cuts at: 0 295 664 873 1068 2280 Size: 295 369 209 195 1212 Fragments arranged by size: 1212 369 295 209 195 EcoO109I rG'GnC_Cy Cuts at: 0 2256 2280 Size: 2256 24 EcoRV GAT'ATC Cuts at: 0 2145 2280 Size: 2145 135 HaeII r_GCGC'y Cuts at: 0 481 1932 2280 Size: 481 1451 348 Fragments arranged by size: 1451 481 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 2248 2280 Size: 187 32 2029 32 Fragments arranged by size: 2029 187 32 32 KpnI G_GTAC'C Cuts at: 0 1743 2280 Size: 1743 537 MfeI C'AATT_G Cuts at: 0 812 2280 Size: 812 1468 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 2280 Size: 386 1894 MscI TGG'CCA Cuts at: 0 1786 2280 Size: 1786 494 MslI CAynn'nnrTG Cuts at: 0 631 1123 1321 1420 1481 1733 2125 2280 Size: 631 492 198 99 61 252 392 155 Fragments arranged by size: 631 492 392 252 198 155 99 61 MspA1I CmG'CkG Cuts at: 0 772 778 1052 1332 1467 2280 Size: 772 6 274 280 135 813 Fragments arranged by size: 813 772 280 274 135 6 NaeI GCC'GGC Cuts at: 0 1298 2051 2280 Size: 1298 753 229 Fragments arranged by size: 1298 753 229 NdeI CA'TA_TG Cuts at: 0 169 528 2234 2280 Size: 169 359 1706 46 Fragments arranged by size: 1706 359 169 46 NgoMIV G'CCGG_C Cuts at: 0 1296 2049 2280 Size: 1296 753 231 Fragments arranged by size: 1296 753 231 NsiI A_TGCA'T Cuts at: 0 1695 2280 Size: 1695 585 PflMI CCAn_nnn'nTGG Cuts at: 0 101 1679 2280 Size: 101 1578 601 Fragments arranged by size: 1578 601 101 PmlI CAC'GTG Cuts at: 0 687 2280 Size: 687 1593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1949 1981 2280 Size: 1949 32 299 Fragments arranged by size: 1949 299 32 PpuMI rG'GwC_Cy Cuts at: 0 2256 2280 Size: 2256 24 PshAI GACnn'nnGTC Cuts at: 0 383 2280 Size: 383 1897 PstI C_TGCA'G Cuts at: 0 777 2280 Size: 777 1503 PvuII CAG'CTG Cuts at: 0 772 1332 1467 2280 Size: 772 560 135 813 Fragments arranged by size: 813 772 560 135 SacI G_AGCT'C Cuts at: 0 1190 2280 Size: 1190 1090 SfcI C'TryA_G Cuts at: 0 773 2280 Size: 773 1507 SmlI C'TyrA_G Cuts at: 0 1222 2280 Size: 1222 1058 StyI C'CwwG_G Cuts at: 0 248 660 2280 Size: 248 412 1620 Fragments arranged by size: 1620 412 248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 201 1852 1900 2280 Size: 201 1651 48 380 Fragments arranged by size: 1651 380 201 48 TatI w'GTAC_w Cuts at: 0 464 731 1640 2021 2280 Size: 464 267 909 381 259 Fragments arranged by size: 909 464 381 267 259 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 200 232 1442 1474 2280 Size: 200 32 1210 32 806 Fragments arranged by size: 1210 806 200 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1528 1596 2280 Size: 1528 68 684 Fragments arranged by size: 1528 684 68 XbaI T'CTAG_A Cuts at: 0 834 2280 Size: 834 1446 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1329 1419 2280 Size: 1329 90 861 Fragments arranged by size: 1329 861 90 XmnI GAAnn'nnTTC Cuts at: 0 1961 2280 Size: 1961 319 ZraI GAC'GTC Cuts at: 0 1794 2280 Size: 1794 486 Enzymes that do cut: AatII Acc65I AcuI AleI AlwNI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KpnI MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NsiI PflMI PmlI PpiI PpuMI PshAI PstI PvuII SacI SfcI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BglII BlpI BmtI BplI BsaBI BsiWI BsmI BspHI BspMI BssHII BssSI BstBI BstEII BstZ17I Bsu36I DraI DraIII DrdI EciI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI MluI NarI NcoI NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XhoI XmaI